A standard for near-scarless plasmid construction using reusable DNA parts

Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.

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Autores principales:	Xiaoqiang Ma, Hong Liang, Xiaoyi Cui, Yurou Liu, Hongyuan Lu, Wenbo Ning, Nga Yu Poon, Benjamin Ho, Kang Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/45ff63c3504f4336b6879639124fe61a
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spelling	oai:doaj.org-article:45ff63c3504f4336b6879639124fe61a2021-12-02T16:57:33ZA standard for near-scarless plasmid construction using reusable DNA parts10.1038/s41467-019-11263-02041-1723https://doaj.org/article/45ff63c3504f4336b6879639124fe61a2019-07-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-11263-0https://doaj.org/toc/2041-1723Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.Xiaoqiang MaHong LiangXiaoyi CuiYurou LiuHongyuan LuWenbo NingNga Yu PoonBenjamin HoKang ZhouNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Xiaoqiang Ma Hong Liang Xiaoyi Cui Yurou Liu Hongyuan Lu Wenbo Ning Nga Yu Poon Benjamin Ho Kang Zhou A standard for near-scarless plasmid construction using reusable DNA parts
description	Construction of plasmids from multiple fragments often uses customised parts and leaves scars where fragments are joined. Here the authors develop a method for barcoding fragments and constructing plasmids in a scarless manner from a collection of standard parts.
format	article
author	Xiaoqiang Ma Hong Liang Xiaoyi Cui Yurou Liu Hongyuan Lu Wenbo Ning Nga Yu Poon Benjamin Ho Kang Zhou
author_facet	Xiaoqiang Ma Hong Liang Xiaoyi Cui Yurou Liu Hongyuan Lu Wenbo Ning Nga Yu Poon Benjamin Ho Kang Zhou
author_sort	Xiaoqiang Ma
title	A standard for near-scarless plasmid construction using reusable DNA parts
title_short	A standard for near-scarless plasmid construction using reusable DNA parts
title_full	A standard for near-scarless plasmid construction using reusable DNA parts
title_fullStr	A standard for near-scarless plasmid construction using reusable DNA parts
title_full_unstemmed	A standard for near-scarless plasmid construction using reusable DNA parts
title_sort	standard for near-scarless plasmid construction using reusable dna parts
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/45ff63c3504f4336b6879639124fe61a
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A standard for near-scarless plasmid construction using reusable DNA parts

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