Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2

<i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins,...

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Autores principales:	Servane Payen, David Roy, Anaïs Boa, Masatoshi Okura, Jean-Philippe Auger, Mariela Segura, Marcelo Gottschalk
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	<i>Streptococcus suis</i> lipoproteins maturation inflammation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/460020f45c674a149a2e483a0c8f5007
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spelling	oai:doaj.org-article:460020f45c674a149a2e483a0c8f50072021-11-25T18:25:35ZRole of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 210.3390/microorganisms91123862076-2607https://doaj.org/article/460020f45c674a149a2e483a0c8f50072021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2386https://doaj.org/toc/2076-2607<i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins, pro-inflammatory cell activators and/or virulence factors. In the current study, we aimed to evaluate the role of the prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein signal peptidase (Lsp) enzymes, which are responsible for LPP maturation, on the pathogenesis of the infection caused by two different sequence types (STs) of <i>S. suis</i> serotype 2 strains (virulent ST1 and highly virulent ST7). Through the use of isogenic Δ<i>lgt</i>, Δ<i>lsp</i> and double Δ<i>lgt</i>/Δ<i>lsp</i> mutants, it was shown that lack of these enzymes did not influence <i>S. suis</i> adhesion/invasion to porcine respiratory epithelial cells. However, in the absence of the Lsp and/or Lgt, a significant reduction in the capacity of <i>S. suis</i> to activate phagocytic cells and induce pro-inflammatory mediators (in vitro and in vivo) was observed. In general, results obtained with the double mutant did not differ in comparison to single mutants, indicating lack of an additive effect. Finally, our data suggest that these enzymes play a differential role in virulence, depending on the genetic background of the strain and being more important for the highly virulent ST7 strain.Servane PayenDavid RoyAnaïs BoaMasatoshi OkuraJean-Philippe AugerMariela SeguraMarcelo GottschalkMDPI AGarticle<i>Streptococcus suis</i>lipoproteinsmaturationinflammationBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2386, p 2386 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	<i>Streptococcus suis</i> lipoproteins maturation inflammation Biology (General) QH301-705.5
spellingShingle	<i>Streptococcus suis</i> lipoproteins maturation inflammation Biology (General) QH301-705.5 Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
description	<i>Streptococcus suis</i> serotype 2 is an important porcine bacterial pathogen associated with multiple pathologies in piglets. Bacterial lipoproteins (LPPs) have been described as playing important roles in the pathogenesis of the infection of other Gram-positive bacteria as adhesins, pro-inflammatory cell activators and/or virulence factors. In the current study, we aimed to evaluate the role of the prolipoprotein diacylglyceryl transferase (Lgt) and lipoprotein signal peptidase (Lsp) enzymes, which are responsible for LPP maturation, on the pathogenesis of the infection caused by two different sequence types (STs) of <i>S. suis</i> serotype 2 strains (virulent ST1 and highly virulent ST7). Through the use of isogenic Δ<i>lgt</i>, Δ<i>lsp</i> and double Δ<i>lgt</i>/Δ<i>lsp</i> mutants, it was shown that lack of these enzymes did not influence <i>S. suis</i> adhesion/invasion to porcine respiratory epithelial cells. However, in the absence of the Lsp and/or Lgt, a significant reduction in the capacity of <i>S. suis</i> to activate phagocytic cells and induce pro-inflammatory mediators (in vitro and in vivo) was observed. In general, results obtained with the double mutant did not differ in comparison to single mutants, indicating lack of an additive effect. Finally, our data suggest that these enzymes play a differential role in virulence, depending on the genetic background of the strain and being more important for the highly virulent ST7 strain.
format	article
author	Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk
author_facet	Servane Payen David Roy Anaïs Boa Masatoshi Okura Jean-Philippe Auger Mariela Segura Marcelo Gottschalk
author_sort	Servane Payen
title	Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
title_short	Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
title_full	Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
title_fullStr	Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
title_full_unstemmed	Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2
title_sort	role of maturation of lipoproteins in the pathogenesis of the infection caused by <i>streptococcus suis</i> serotype 2
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/460020f45c674a149a2e483a0c8f5007
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Role of Maturation of Lipoproteins in the Pathogenesis of the Infection Caused by <i>Streptococcus suis</i> Serotype 2

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