Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets

It has been shown that enzyme-treated plant protein can increase performance and promote intestinal health, and save dietary protein. However, our understanding of the effects of enzyme-treated soy protein on performance and intestine function in laying hens, and its rational use, remains limited. T...

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Autores principales:	X.M. Ding, P. Liu, K.Y. Zhang, J.P. Wang, S.P. Bai, Q.F. Zeng, Y. Xuan, Z.W. Su, H.W. Peng, D.D. Li
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Egg production Intestine function Layers Nutrient level Soybean protein Animal culture SF1-1100
Acceso en línea:	https://doaj.org/article/46029dcdaab14a84b9e66b84654b66d9
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spelling	oai:doaj.org-article:46029dcdaab14a84b9e66b84654b66d92021-11-26T04:25:11ZEffects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets1751-731110.1016/j.animal.2021.100373https://doaj.org/article/46029dcdaab14a84b9e66b84654b66d92021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1751731121002160https://doaj.org/toc/1751-7311It has been shown that enzyme-treated plant protein can increase performance and promote intestinal health, and save dietary protein. However, our understanding of the effects of enzyme-treated soy protein on performance and intestine function in laying hens, and its rational use, remains limited. The experiment was conducted to study the effect of enzyme-treated soy protein (ETSP) in different nutrient density diets on performance, egg quality, digestive enzyme activity and mRNA expression of amino acid transporters of laying hens. A total of 1 200 Lohmann laying hens (52 wk of age) was randomly divided into a 3 × 2 factorial design that included three nutrient levels: [positive control (PC), metabolisable energy (ME): 2 680 kcal/kg, CP: 15.5%; negative control 1 (NC1), ME: 2 630 kcal/kg, CP: 15%; negative control 2 (NC2), ME:2 580 kcal/kg, CP: 14.5%] and 2 ETSP levels (0 and 0.5%) for 12 weeks. Each treatment had 10 replicates with 20 birds. With the decrease of dietary nutrition density, egg production rate (P = 0.07) and feed conversion ratio (FCR) (P = 0.06) were reduced. Yolk colour was decreased, and yolk index was increased. Supplemented ETSP improved FCR (P = 0.05) and qualified egg rate (P < 0.05). The mass loss rate of egg was decreased after storage for 30 days (P < 0.05). An interaction between nutrient density and ETSP was observed on albumen height and Haugh unit (P < 0.05), and the effects were most noticeable in hens fed 0.5% ETSP in NC2 group. An increase in the activity of trypsin in duodenum (P < 0.05) and the relative expressions of jejunum peptide transporter 1 (PepT1) (P < 0.05) and B0 system neutral amino acid transport carrier (B0AT) mRNA (P < 0.01) was observed during ETSP supplementation. The nutrient density and ETSP supplementation had no significant effect on microbiota in the cecal contents. Overall, the results in this study indicated that the ME decreased 100 kcal/kg and CP decreased 1% in diet of laying hens had a decreasing trend on production performance, no effects on enzyme activity, amino acid transporter mRNA, and gut microbiota, whereas 0.5% ETSP can increase activity of trypsin, PepT1 and B0AT mRNA relative expressions, and improve FCR, qualified egg rate.X.M. DingP. LiuK.Y. ZhangJ.P. WangS.P. BaiQ.F. ZengY. XuanZ.W. SuH.W. PengD.D. LiElsevierarticleEgg productionIntestine functionLayersNutrient levelSoybean proteinAnimal cultureSF1-1100ENAnimal, Vol 15, Iss 11, Pp 100373- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Egg production Intestine function Layers Nutrient level Soybean protein Animal culture SF1-1100
spellingShingle	Egg production Intestine function Layers Nutrient level Soybean protein Animal culture SF1-1100 X.M. Ding P. Liu K.Y. Zhang J.P. Wang S.P. Bai Q.F. Zeng Y. Xuan Z.W. Su H.W. Peng D.D. Li Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
description	It has been shown that enzyme-treated plant protein can increase performance and promote intestinal health, and save dietary protein. However, our understanding of the effects of enzyme-treated soy protein on performance and intestine function in laying hens, and its rational use, remains limited. The experiment was conducted to study the effect of enzyme-treated soy protein (ETSP) in different nutrient density diets on performance, egg quality, digestive enzyme activity and mRNA expression of amino acid transporters of laying hens. A total of 1 200 Lohmann laying hens (52 wk of age) was randomly divided into a 3 × 2 factorial design that included three nutrient levels: [positive control (PC), metabolisable energy (ME): 2 680 kcal/kg, CP: 15.5%; negative control 1 (NC1), ME: 2 630 kcal/kg, CP: 15%; negative control 2 (NC2), ME:2 580 kcal/kg, CP: 14.5%] and 2 ETSP levels (0 and 0.5%) for 12 weeks. Each treatment had 10 replicates with 20 birds. With the decrease of dietary nutrition density, egg production rate (P = 0.07) and feed conversion ratio (FCR) (P = 0.06) were reduced. Yolk colour was decreased, and yolk index was increased. Supplemented ETSP improved FCR (P = 0.05) and qualified egg rate (P < 0.05). The mass loss rate of egg was decreased after storage for 30 days (P < 0.05). An interaction between nutrient density and ETSP was observed on albumen height and Haugh unit (P < 0.05), and the effects were most noticeable in hens fed 0.5% ETSP in NC2 group. An increase in the activity of trypsin in duodenum (P < 0.05) and the relative expressions of jejunum peptide transporter 1 (PepT1) (P < 0.05) and B0 system neutral amino acid transport carrier (B0AT) mRNA (P < 0.01) was observed during ETSP supplementation. The nutrient density and ETSP supplementation had no significant effect on microbiota in the cecal contents. Overall, the results in this study indicated that the ME decreased 100 kcal/kg and CP decreased 1% in diet of laying hens had a decreasing trend on production performance, no effects on enzyme activity, amino acid transporter mRNA, and gut microbiota, whereas 0.5% ETSP can increase activity of trypsin, PepT1 and B0AT mRNA relative expressions, and improve FCR, qualified egg rate.
format	article
author	X.M. Ding P. Liu K.Y. Zhang J.P. Wang S.P. Bai Q.F. Zeng Y. Xuan Z.W. Su H.W. Peng D.D. Li
author_facet	X.M. Ding P. Liu K.Y. Zhang J.P. Wang S.P. Bai Q.F. Zeng Y. Xuan Z.W. Su H.W. Peng D.D. Li
author_sort	X.M. Ding
title	Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
title_short	Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
title_full	Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
title_fullStr	Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
title_full_unstemmed	Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets
title_sort	effects of enzyme-treated soy protein on performance, digestive enzyme activity and mrna expression of nutrient transporters of laying hens fed different nutrient density diets
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/46029dcdaab14a84b9e66b84654b66d9
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Effects of enzyme-treated soy protein on performance, digestive enzyme activity and mRNA expression of nutrient transporters of laying hens fed different nutrient density diets

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