Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin.
<h4>Background</h4>To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene bla(NDM-1) in bacteria of food animal origin.<h4>Methodology/principal findings</h4>Gram-negative bacteria with low suscepti...
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2012
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oai:doaj.org-article:46036ad48d6844d39a40431cd60215042021-11-18T07:18:01ZIdentification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin.1932-620310.1371/journal.pone.0037152https://doaj.org/article/46036ad48d6844d39a40431cd60215042012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22629360/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene bla(NDM-1) in bacteria of food animal origin.<h4>Methodology/principal findings</h4>Gram-negative bacteria with low susceptibility to imipenem (MIC>8 µg/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern China. These bacteria were selected for phenotypic and molecular detection of known MBL genes and antimicrobial susceptibility testing. For the bla(NDM-1) positive isolate, conjugation and transformation experiments were carried out to assess plasmid transfer. Southern blotting was conducted to localize the bla(NDM-1) genes, and DNA sequencing was performed to determine the sequences of bla(NDM-1) and the flanking genes. In total, nine gram-negative bacteria of four different species presented a MBL phenotype. bla(NDM-1) was identified on a mobile plasmid named pAL-01 in an Acinetobacter lwoffii isolate of chicken origin. Transfer of pAL-01 from this isolate to E. coli J53 and JM109 resulted in resistance to multiple β-lactams. Sequence analysis revealed that the bla(NDM-1) gene is attached to an intact insertion element ISAba125, whose right inverted repeat (IR-R) overlaps with the promoter sequence of bla(NDM-1). Thus, insertion of ISAba125 likely enhances the expression of bla(NDM-1).<h4>Conclusion</h4>The identification of a bla(NDM-1)- carrying strain of A. lwoffii in chickens suggests the potential for zoonotic transmission of bla(NDM-1) and has important implications for food safety.Yang WangCongming WuQijing ZhangJing QiHebing LiuYu WangTao HeLicai MaJing LaiZhangqi ShenYuqing LiuJianzhong ShenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 5, p e37152 (2012) |
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Medicine R Science Q Yang Wang Congming Wu Qijing Zhang Jing Qi Hebing Liu Yu Wang Tao He Licai Ma Jing Lai Zhangqi Shen Yuqing Liu Jianzhong Shen Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| description |
<h4>Background</h4>To investigate the presence of metallo-β-lactamase (MBL) genes and the genetic environment of the New Delhi metallo-β-lactamase gene bla(NDM-1) in bacteria of food animal origin.<h4>Methodology/principal findings</h4>Gram-negative bacteria with low susceptibility to imipenem (MIC>8 µg/mL) were isolated from swab samples collected from 15 animal farms and one slaughterhouse in eastern China. These bacteria were selected for phenotypic and molecular detection of known MBL genes and antimicrobial susceptibility testing. For the bla(NDM-1) positive isolate, conjugation and transformation experiments were carried out to assess plasmid transfer. Southern blotting was conducted to localize the bla(NDM-1) genes, and DNA sequencing was performed to determine the sequences of bla(NDM-1) and the flanking genes. In total, nine gram-negative bacteria of four different species presented a MBL phenotype. bla(NDM-1) was identified on a mobile plasmid named pAL-01 in an Acinetobacter lwoffii isolate of chicken origin. Transfer of pAL-01 from this isolate to E. coli J53 and JM109 resulted in resistance to multiple β-lactams. Sequence analysis revealed that the bla(NDM-1) gene is attached to an intact insertion element ISAba125, whose right inverted repeat (IR-R) overlaps with the promoter sequence of bla(NDM-1). Thus, insertion of ISAba125 likely enhances the expression of bla(NDM-1).<h4>Conclusion</h4>The identification of a bla(NDM-1)- carrying strain of A. lwoffii in chickens suggests the potential for zoonotic transmission of bla(NDM-1) and has important implications for food safety. |
| format |
article |
| author |
Yang Wang Congming Wu Qijing Zhang Jing Qi Hebing Liu Yu Wang Tao He Licai Ma Jing Lai Zhangqi Shen Yuqing Liu Jianzhong Shen |
| author_facet |
Yang Wang Congming Wu Qijing Zhang Jing Qi Hebing Liu Yu Wang Tao He Licai Ma Jing Lai Zhangqi Shen Yuqing Liu Jianzhong Shen |
| author_sort |
Yang Wang |
| title |
Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| title_short |
Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| title_full |
Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| title_fullStr |
Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| title_full_unstemmed |
Identification of New Delhi metallo-β-lactamase 1 in Acinetobacter lwoffii of food animal origin. |
| title_sort |
identification of new delhi metallo-β-lactamase 1 in acinetobacter lwoffii of food animal origin. |
| publisher |
Public Library of Science (PLoS) |
| publishDate |
2012 |
| url |
https://doaj.org/article/46036ad48d6844d39a40431cd6021504 |
| work_keys_str_mv |
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| _version_ |
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