Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful...

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Autores principales:	Meredith H Wilson, Stephen C Ekker, Steven A Farber
Formato:	article
Lenguaje:	EN
Publicado:	eLife Sciences Publications Ltd 2021
Materias:	lipid droplet perilipin PLIN Medicine R Science Q Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/46058fdd397a40a9828b3b8699040bfe
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spelling	oai:doaj.org-article:46058fdd397a40a9828b3b8699040bfe2021-11-15T07:01:45ZImaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish10.7554/eLife.663932050-084Xe66393https://doaj.org/article/46058fdd397a40a9828b3b8699040bfe2021-08-01T00:00:00Zhttps://elifesciences.org/articles/66393https://doaj.org/toc/2050-084XCytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real time from the subcellular to the whole organism level. Fluorescently tagging the lipid droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.Meredith H WilsonStephen C EkkerSteven A FarbereLife Sciences Publications Ltdarticlelipid dropletperilipinPLINMedicineRScienceQBiology (General)QH301-705.5ENeLife, Vol 10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lipid droplet perilipin PLIN Medicine R Science Q Biology (General) QH301-705.5
spellingShingle	lipid droplet perilipin PLIN Medicine R Science Q Biology (General) QH301-705.5 Meredith H Wilson Stephen C Ekker Steven A Farber Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
description	Cytoplasmic lipid droplets are highly dynamic storage organelles that are critical for cellular lipid homeostasis. While the molecular details of lipid droplet dynamics are a very active area of investigation, this work has been primarily performed in cultured cells. Taking advantage of the powerful transgenic and in vivo imaging opportunities available in zebrafish, we built a suite of tools to study lipid droplets in real time from the subcellular to the whole organism level. Fluorescently tagging the lipid droplet-associated proteins, perilipin 2 and perilipin 3, in the endogenous loci permits visualization of lipid droplets in the intestine, liver, and adipose tissue. Using these tools, we found that perilipin 3 is rapidly loaded on intestinal lipid droplets following a high-fat meal and later replaced by perilipin 2. These powerful new tools will facilitate studies on the role of lipid droplets in different tissues, under different genetic and physiological manipulations, and in a variety of human disease models.
format	article
author	Meredith H Wilson Stephen C Ekker Steven A Farber
author_facet	Meredith H Wilson Stephen C Ekker Steven A Farber
author_sort	Meredith H Wilson
title	Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
title_short	Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
title_full	Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
title_fullStr	Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
title_full_unstemmed	Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
title_sort	imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish
publisher	eLife Sciences Publications Ltd
publishDate	2021
url	https://doaj.org/article/46058fdd397a40a9828b3b8699040bfe
work_keys_str_mv	AT meredithhwilson imagingcytoplasmiclipiddropletsinvivowithfluorescentperilipin2andperilipin3knockinzebrafish AT stephencekker imagingcytoplasmiclipiddropletsinvivowithfluorescentperilipin2andperilipin3knockinzebrafish AT stevenafarber imagingcytoplasmiclipiddropletsinvivowithfluorescentperilipin2andperilipin3knockinzebrafish
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Imaging cytoplasmic lipid droplets in vivo with fluorescent perilipin 2 and perilipin 3 knock-in zebrafish

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