Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant

Abstract Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was as...

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Autores principales:	Angela Lemke, José Carlos Castillo-Sánchez, Florian Prodinger, Asja Ceranic, Simone Hennerbichler-Lugscheider, Jesús Pérez-Gil, Heinz Redl, Susanne Wolbank
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46083635f5674c7f92ba6172c47d86dc
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spelling	oai:doaj.org-article:46083635f5674c7f92ba6172c47d86dc2021-12-02T16:07:05ZHuman amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant10.1038/s41598-017-06402-w2045-2322https://doaj.org/article/46083635f5674c7f92ba6172c47d86dc2017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06402-whttps://doaj.org/toc/2045-2322Abstract Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fluid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fluid. We identified that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profiling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fluid aside from the foetal lung.Angela LemkeJosé Carlos Castillo-SánchezFlorian ProdingerAsja CeranicSimone Hennerbichler-LugscheiderJesús Pérez-GilHeinz RedlSusanne WolbankNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Angela Lemke José Carlos Castillo-Sánchez Florian Prodinger Asja Ceranic Simone Hennerbichler-Lugscheider Jesús Pérez-Gil Heinz Redl Susanne Wolbank Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
description	Abstract Pulmonary surfactant (PS) reduces surface tension at the air-liquid interface in the alveolar epithelium of the lung, which is required for breathing and for the pulmonary maturity of the developing foetus. However, the origin of PS had never been thoroughly investigated, although it was assumed to be secreted from the foetal developing lung. Human amniotic membrane (hAM), particularly its epithelial cell layer, composes the amniotic sac enclosing the amniotic fluid. In this study, we therefore aimed to investigate a potential contribution of the cellular components of the hAM to pulmonary surfactant found in amniotic fluid. We identified that cells within the native membrane contain lamellar bodies and express all four surfactant proteins as well as ABCA3. Lipidomic profiling by nanoESI – MS/MS revealed the presence of the essential lipid species as found in PS. Also, the biophysical activity of conditioned cell culture supernatant obtained from hAM was tested with captive bubble surfactometry. hAM supernatant showed the ability to reduce surface tension, similar to human PS obtained from bronchoalveolar lavage. This means that hAM produces the essential PS-associated components and can therefore contribute as second potential source of PS in amniotic fluid aside from the foetal lung.
format	article
author	Angela Lemke José Carlos Castillo-Sánchez Florian Prodinger Asja Ceranic Simone Hennerbichler-Lugscheider Jesús Pérez-Gil Heinz Redl Susanne Wolbank
author_facet	Angela Lemke José Carlos Castillo-Sánchez Florian Prodinger Asja Ceranic Simone Hennerbichler-Lugscheider Jesús Pérez-Gil Heinz Redl Susanne Wolbank
author_sort	Angela Lemke
title	Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
title_short	Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
title_full	Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
title_fullStr	Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
title_full_unstemmed	Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
title_sort	human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/46083635f5674c7f92ba6172c47d86dc
work_keys_str_mv	AT angelalemke humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT josecarloscastillosanchez humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT florianprodinger humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT asjaceranic humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT simonehennerbichlerlugscheider humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT jesusperezgil humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT heinzredl humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant AT susannewolbank humanamnioticmembraneasnewlyidentifiedsourceofamnioticfluidpulmonarysurfactant
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Human amniotic membrane as newly identified source of amniotic fluid pulmonary surfactant

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