Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020. Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. ?-Glucosidase is the family of glycosyl hydrolase that have potential role in various fo...

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Autores principales:	Sri Sugiwati, Muhammad Hanafi, Hanifah Lioe, Maggy Suhartono
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	aspergillus niger, fermentation condition, -glucosidase, rice bran, solid-state fermentation Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/46097387563349a5a039ac99b4340971
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spelling	oai:doaj.org-article:46097387563349a5a039ac99b43409712021-11-22T00:41:43ZEffect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate1412-033X2085-472210.13057/biodiv/d210917https://doaj.org/article/46097387563349a5a039ac99b43409712020-08-01T00:00:00Zhttps://smujo.id/biodiv/article/view/6130https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020. Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. ?-Glucosidase is the family of glycosyl hydrolase that have potential role in various food industry, such as in tea, wine and vanilla industries to increase the aroma and production of isoflavone aglycons in soybean flour. The present work produced ?-glucosidase from local isolate of Aspergillus niger InaCC F57 under solid-state fermentation (SSF) using rice bran substrate. Fermentation process was made in various conditions with respect to carbon source as substrate, initial pH of fermentation medium, incubation time, water to substrate ratio, fermentation temperature, and addition of Mandels mineral salts solution. The results showed that activity of ?-glucosidase was best at, i.e., 2.45 U/mL, with the use of rice bran as substrate. Furthermore, optimum condition for the highest production of ?-glucosidase occurred at pH 2.0, incubation time of 5 days, water to substrate ratio of 1.5: 1, and incubation temperature of 32°C. Additionally, in optimum fermentation conditions, production of ?-glucosidase could be enhanced up to 26.22% with the presence of Mandels mineral salts solution as compared to the control.Sri SugiwatiMuhammad HanafiHanifah LioeMaggy SuhartonoMBI & UNS Soloarticleaspergillus niger, fermentation condition, -glucosidase, rice bran, solid-state fermentationBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	aspergillus niger, fermentation condition, -glucosidase, rice bran, solid-state fermentation Biology (General) QH301-705.5
spellingShingle	aspergillus niger, fermentation condition, -glucosidase, rice bran, solid-state fermentation Biology (General) QH301-705.5 Sri Sugiwati Muhammad Hanafi Hanifah Lioe Maggy Suhartono Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
description	Abstract. Sugiwati S, Hanafi M, Lioe HN, Suhartono MT. 2020. Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate. Biodiversitas 21: 4058-4066. ?-Glucosidase is the family of glycosyl hydrolase that have potential role in various food industry, such as in tea, wine and vanilla industries to increase the aroma and production of isoflavone aglycons in soybean flour. The present work produced ?-glucosidase from local isolate of Aspergillus niger InaCC F57 under solid-state fermentation (SSF) using rice bran substrate. Fermentation process was made in various conditions with respect to carbon source as substrate, initial pH of fermentation medium, incubation time, water to substrate ratio, fermentation temperature, and addition of Mandels mineral salts solution. The results showed that activity of ?-glucosidase was best at, i.e., 2.45 U/mL, with the use of rice bran as substrate. Furthermore, optimum condition for the highest production of ?-glucosidase occurred at pH 2.0, incubation time of 5 days, water to substrate ratio of 1.5: 1, and incubation temperature of 32°C. Additionally, in optimum fermentation conditions, production of ?-glucosidase could be enhanced up to 26.22% with the presence of Mandels mineral salts solution as compared to the control.
format	article
author	Sri Sugiwati Muhammad Hanafi Hanifah Lioe Maggy Suhartono
author_facet	Sri Sugiwati Muhammad Hanafi Hanifah Lioe Maggy Suhartono
author_sort	Sri Sugiwati
title	Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
title_short	Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
title_full	Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
title_fullStr	Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
title_full_unstemmed	Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate
title_sort	effect of growth conditions on ?-glucosidase production by local isolate of aspergillus niger using rice bran substrate
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/46097387563349a5a039ac99b4340971
work_keys_str_mv	AT srisugiwati effectofgrowthconditionsonglucosidaseproductionbylocalisolateofaspergillusnigerusingricebransubstrate AT muhammadhanafi effectofgrowthconditionsonglucosidaseproductionbylocalisolateofaspergillusnigerusingricebransubstrate AT hanifahlioe effectofgrowthconditionsonglucosidaseproductionbylocalisolateofaspergillusnigerusingricebransubstrate AT maggysuhartono effectofgrowthconditionsonglucosidaseproductionbylocalisolateofaspergillusnigerusingricebransubstrate
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Effect of growth conditions on ?-glucosidase production by local isolate of Aspergillus niger using rice bran substrate

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