Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits

ABSTRACT Vibrio anguillarum is a marine bacterium that can cause vibriosis in many fish and shellfish species, leading to high mortalities and economic losses in aquaculture. Although putative virulence factors have been identified, the mechanism of pathogenesis of V. anguillarum is not fully unders...

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Autores principales:	Daniel Castillo, Paul D. Alvise, Ruiqi Xu, Faxing Zhang, Mathias Middelboe, Lone Gram
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2017
Materias:	pan-genome genomics virulence factors Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4611ccf415ae48978d46ce48c5bc2c86
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spelling	oai:doaj.org-article:4611ccf415ae48978d46ce48c5bc2c862021-12-02T19:47:35ZComparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits10.1128/mSystems.00001-172379-5077https://doaj.org/article/4611ccf415ae48978d46ce48c5bc2c862017-02-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSystems.00001-17https://doaj.org/toc/2379-5077ABSTRACT Vibrio anguillarum is a marine bacterium that can cause vibriosis in many fish and shellfish species, leading to high mortalities and economic losses in aquaculture. Although putative virulence factors have been identified, the mechanism of pathogenesis of V. anguillarum is not fully understood. Here, we analyzed whole-genome sequences of a collection of V. anguillarum strains and compared them to virulence of the strains as determined in larval challenge assays. Previously identified virulence factors were globally distributed among the strains, with some genetic diversity. However, the pan-genome revealed that six out of nine high-virulence strains possessed a unique accessory genome that was attributed to pathogenic genomic islands, prophage-like elements, virulence factors, and a new set of gene clusters involved in biosynthesis, modification, and transport of polysaccharides. In contrast, V. anguillarum strains that were medium to nonvirulent had a high degree of genomic homogeneity. Finally, we found that a phylogeny based on the core genomes clustered the strains with moderate to no virulence, while six out of nine high-virulence strains represented phylogenetically separate clusters. Hence, we suggest a link between genotype and virulence characteristics of Vibrio anguillarum, which can be used to unravel the molecular evolution of V. anguillarum and can also be important from survey and diagnostic perspectives. IMPORTANCE Comparative genome analysis of strains of a pathogenic bacterial species can be a powerful tool to discover acquisition of mobile genetic elements related to virulence. Here, we compared 28 V. anguillarum strains that differed in virulence in fish larval models. By pan-genome analyses, we found that six of nine highly virulent strains had a unique core and accessory genome. In contrast, V. anguillarum strains that were medium to nonvirulent had low genomic diversity. Integration of genomic and phenotypic features provides insights into the evolution of V. anguillarum and can also be important for survey and diagnostic purposes.Daniel CastilloPaul D. AlviseRuiqi XuFaxing ZhangMathias MiddelboeLone GramAmerican Society for Microbiologyarticlepan-genomegenomicsvirulence factorsMicrobiologyQR1-502ENmSystems, Vol 2, Iss 1 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	pan-genome genomics virulence factors Microbiology QR1-502
spellingShingle	pan-genome genomics virulence factors Microbiology QR1-502 Daniel Castillo Paul D. Alvise Ruiqi Xu Faxing Zhang Mathias Middelboe Lone Gram Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
description	ABSTRACT Vibrio anguillarum is a marine bacterium that can cause vibriosis in many fish and shellfish species, leading to high mortalities and economic losses in aquaculture. Although putative virulence factors have been identified, the mechanism of pathogenesis of V. anguillarum is not fully understood. Here, we analyzed whole-genome sequences of a collection of V. anguillarum strains and compared them to virulence of the strains as determined in larval challenge assays. Previously identified virulence factors were globally distributed among the strains, with some genetic diversity. However, the pan-genome revealed that six out of nine high-virulence strains possessed a unique accessory genome that was attributed to pathogenic genomic islands, prophage-like elements, virulence factors, and a new set of gene clusters involved in biosynthesis, modification, and transport of polysaccharides. In contrast, V. anguillarum strains that were medium to nonvirulent had a high degree of genomic homogeneity. Finally, we found that a phylogeny based on the core genomes clustered the strains with moderate to no virulence, while six out of nine high-virulence strains represented phylogenetically separate clusters. Hence, we suggest a link between genotype and virulence characteristics of Vibrio anguillarum, which can be used to unravel the molecular evolution of V. anguillarum and can also be important from survey and diagnostic perspectives. IMPORTANCE Comparative genome analysis of strains of a pathogenic bacterial species can be a powerful tool to discover acquisition of mobile genetic elements related to virulence. Here, we compared 28 V. anguillarum strains that differed in virulence in fish larval models. By pan-genome analyses, we found that six of nine highly virulent strains had a unique core and accessory genome. In contrast, V. anguillarum strains that were medium to nonvirulent had low genomic diversity. Integration of genomic and phenotypic features provides insights into the evolution of V. anguillarum and can also be important for survey and diagnostic purposes.
format	article
author	Daniel Castillo Paul D. Alvise Ruiqi Xu Faxing Zhang Mathias Middelboe Lone Gram
author_facet	Daniel Castillo Paul D. Alvise Ruiqi Xu Faxing Zhang Mathias Middelboe Lone Gram
author_sort	Daniel Castillo
title	Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
title_short	Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
title_full	Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
title_fullStr	Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
title_full_unstemmed	Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits
title_sort	comparative genome analyses of <italic toggle="yes">vibrio anguillarum</italic> strains reveal a link with pathogenicity traits
publisher	American Society for Microbiology
publishDate	2017
url	https://doaj.org/article/4611ccf415ae48978d46ce48c5bc2c86
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Comparative Genome Analyses of <italic toggle="yes">Vibrio anguillarum</italic> Strains Reveal a Link with Pathogenicity Traits

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