Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.

Alteration of cancer cell toward mesenchymal phenotype has been shown to potentiate tumor aggressiveness by increasing cancer cell metastasis. Herein, we report the effect of triclosan, a widely used antibacterial agent found in many daily products, in enhancing the epithelial-to-mesenchymal transit...

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Autores principales:	Thidarat Winitthana, Somsong Lawanprasert, Pithi Chanvorachote
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4619814547c542fcb0ba6e90225e6f2e
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spelling	oai:doaj.org-article:4619814547c542fcb0ba6e90225e6f2e2021-11-25T05:56:22ZTriclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.1932-620310.1371/journal.pone.0110851https://doaj.org/article/4619814547c542fcb0ba6e90225e6f2e2014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0110851https://doaj.org/toc/1932-6203Alteration of cancer cell toward mesenchymal phenotype has been shown to potentiate tumor aggressiveness by increasing cancer cell metastasis. Herein, we report the effect of triclosan, a widely used antibacterial agent found in many daily products, in enhancing the epithelial-to-mesenchymal transition (EMT) in aggressive anoikis resistant human H460 lung cancer cells. EMT has been long known to increase abilities of the cells to increase migration, invasion, and survival in circulating system. The present study reveals that treatment of the cancer cells with triclosan at the physiologically related concentrations significantly increased the colony number of the cancer cells assessed by tumor formation assay. Also, the mesenchymal-like morphology and decrease in cell-to-cell adhesion were observed in triclosan-treated cells. Importantly, western blot analysis revealed that triclosan-treated cells exhibited decreased E-cadherin, while the levels of EMT markers, namely N-cadherin, vimentin, snail and slug were found to be significantly up-regulated. Furthermore, EMT induced by triclosan treatment was accompanied by the activation of focal adhesion kinase/ATP dependent tyrosine kinase (FAK/Akt) and Ras-related C3 botulinum toxin substrate 1 (Rac1), which enhanced the ability of the cells to migrate and invade. In conclusion, we demonstrated for the first time that triclosan may potentiate cancer cells survival in detached condition and motility via the process of EMT. As mentioned capabilities are required for success in metastasis, the present study provides the novel toxicological information and encourages the awareness of triclosan use in cancer patients.Thidarat WinitthanaSomsong LawanprasertPithi ChanvorachotePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e110851 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Thidarat Winitthana Somsong Lawanprasert Pithi Chanvorachote Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
description	Alteration of cancer cell toward mesenchymal phenotype has been shown to potentiate tumor aggressiveness by increasing cancer cell metastasis. Herein, we report the effect of triclosan, a widely used antibacterial agent found in many daily products, in enhancing the epithelial-to-mesenchymal transition (EMT) in aggressive anoikis resistant human H460 lung cancer cells. EMT has been long known to increase abilities of the cells to increase migration, invasion, and survival in circulating system. The present study reveals that treatment of the cancer cells with triclosan at the physiologically related concentrations significantly increased the colony number of the cancer cells assessed by tumor formation assay. Also, the mesenchymal-like morphology and decrease in cell-to-cell adhesion were observed in triclosan-treated cells. Importantly, western blot analysis revealed that triclosan-treated cells exhibited decreased E-cadherin, while the levels of EMT markers, namely N-cadherin, vimentin, snail and slug were found to be significantly up-regulated. Furthermore, EMT induced by triclosan treatment was accompanied by the activation of focal adhesion kinase/ATP dependent tyrosine kinase (FAK/Akt) and Ras-related C3 botulinum toxin substrate 1 (Rac1), which enhanced the ability of the cells to migrate and invade. In conclusion, we demonstrated for the first time that triclosan may potentiate cancer cells survival in detached condition and motility via the process of EMT. As mentioned capabilities are required for success in metastasis, the present study provides the novel toxicological information and encourages the awareness of triclosan use in cancer patients.
format	article
author	Thidarat Winitthana Somsong Lawanprasert Pithi Chanvorachote
author_facet	Thidarat Winitthana Somsong Lawanprasert Pithi Chanvorachote
author_sort	Thidarat Winitthana
title	Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
title_short	Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
title_full	Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
title_fullStr	Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
title_full_unstemmed	Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
title_sort	triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/4619814547c542fcb0ba6e90225e6f2e
work_keys_str_mv	AT thidaratwinitthana triclosanpotentiatesepithelialtomesenchymaltransitioninanoikisresistanthumanlungcancercells AT somsonglawanprasert triclosanpotentiatesepithelialtomesenchymaltransitioninanoikisresistanthumanlungcancercells AT pithichanvorachote triclosanpotentiatesepithelialtomesenchymaltransitioninanoikisresistanthumanlungcancercells
_version_	1718414334713921536

Triclosan potentiates epithelial-to-mesenchymal transition in anoikis-resistant human lung cancer cells.

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