Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation

An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Ma...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	D P Prakash, Y L Ramachandra, Vageeshbabu S Hanur
Formato:	article
Lenguaje:	EN
Publicado:	Society for Promotion of Horticulture - Indian Institute of Horticultural Research 2015
Materias:	eggplant pre-culture on high bap explant characters pcr Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/461af85bfa8a4c0387bae999612e4909
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:461af85bfa8a4c0387bae999612e4909
record_format	dspace
spelling	oai:doaj.org-article:461af85bfa8a4c0387bae999612e49092021-12-02T11:06:50ZFactors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation0973-354X2582-4899https://doaj.org/article/461af85bfa8a4c0387bae999612e49092015-12-01T00:00:00Zhttps://jhs.iihr.res.in/index.php/jhs/article/view/119https://doaj.org/toc/0973-354Xhttps://doaj.org/toc/2582-4899An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Manjarigota, Agrobacterium strain A208 and shoot regeneration medium (full-strength basal MS medium, 2μM BAP + 0.05μM NAA, 3% sucrose and 0.8% agar) containing Cefotaxime (250-500mg l-1) and Kanamycin (100mg l-1). Hypocotyl explants showed callus initiation and shoot regeneration response after 10-12 and 20-22 days of culture, respectively. Five-day-old explants did not survive Agrobacterium infection, and ten-day-old explants showed higher shoot regeneration (29±1.91%) than older explants. Explants of medium size (1cm long; 32±2.62%) from the apical region (38.57±2.61%) showed better shootregeneration ability than explants of any other size or region. A period of four days of pre-culture (33.33±3.76) was optimal best for best shoot-regeneration in hypocotyl explants. No regeneration was seen in hypocotyl explants at shorter or longer pre-culture period. High cytokinin (10μM) in shoot regeneration medium during pre-culture enhanced shoot regeneration response (47.27±2.98%) in explants co-cultivated with Agrobacterium. Effects of various factors documented in this study will be useful in developing an efficient Agrobacterium-mediated transformation protocol in brinjal cv. Manjarigota.D P PrakashY L RamachandraVageeshbabu S HanurSociety for Promotion of Horticulture - Indian Institute of Horticultural Researcharticleeggplantpre-culture on high bapexplant characterspcrPlant cultureSB1-1110ENJournal of Horticultural Sciences, Vol 10, Iss 2, Pp 136-142 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	eggplant pre-culture on high bap explant characters pcr Plant culture SB1-1110
spellingShingle	eggplant pre-culture on high bap explant characters pcr Plant culture SB1-1110 D P Prakash Y L Ramachandra Vageeshbabu S Hanur Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
description	An attempt was made to assess the effect of size, age and position of the explant, pre-culture and high cytokinin concentration in the pre-culture medium on shoot regeneration in brinjal hypocotyls co-cultivated with Agrobacterium. The study was carried out using hypocotyl explants of brinjal cv. Manjarigota, Agrobacterium strain A208 and shoot regeneration medium (full-strength basal MS medium, 2μM BAP + 0.05μM NAA, 3% sucrose and 0.8% agar) containing Cefotaxime (250-500mg l-1) and Kanamycin (100mg l-1). Hypocotyl explants showed callus initiation and shoot regeneration response after 10-12 and 20-22 days of culture, respectively. Five-day-old explants did not survive Agrobacterium infection, and ten-day-old explants showed higher shoot regeneration (29±1.91%) than older explants. Explants of medium size (1cm long; 32±2.62%) from the apical region (38.57±2.61%) showed better shootregeneration ability than explants of any other size or region. A period of four days of pre-culture (33.33±3.76) was optimal best for best shoot-regeneration in hypocotyl explants. No regeneration was seen in hypocotyl explants at shorter or longer pre-culture period. High cytokinin (10μM) in shoot regeneration medium during pre-culture enhanced shoot regeneration response (47.27±2.98%) in explants co-cultivated with Agrobacterium. Effects of various factors documented in this study will be useful in developing an efficient Agrobacterium-mediated transformation protocol in brinjal cv. Manjarigota.
format	article
author	D P Prakash Y L Ramachandra Vageeshbabu S Hanur
author_facet	D P Prakash Y L Ramachandra Vageeshbabu S Hanur
author_sort	D P Prakash
title	Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
title_short	Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
title_full	Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
title_fullStr	Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
title_full_unstemmed	Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation
title_sort	factors affecting in vitro shoot regeneration in hypocotyls of brinjal (solanum melongena l.) in the early steps of agrobacterium-mediated transformation
publisher	Society for Promotion of Horticulture - Indian Institute of Horticultural Research
publishDate	2015
url	https://doaj.org/article/461af85bfa8a4c0387bae999612e4909
work_keys_str_mv	AT dpprakash factorsaffectinginvitroshootregenerationinhypocotylsofbrinjalsolanummelongenalintheearlystepsofagrobacteriummediatedtransformation AT ylramachandra factorsaffectinginvitroshootregenerationinhypocotylsofbrinjalsolanummelongenalintheearlystepsofagrobacteriummediatedtransformation AT vageeshbabushanur factorsaffectinginvitroshootregenerationinhypocotylsofbrinjalsolanummelongenalintheearlystepsofagrobacteriummediatedtransformation
_version_	1718396222812717056

Factors Affecting in Vitro Shoot Regeneration in Hypocotyls of Brinjal (Solanum melongena L.) in the Early Steps of Agrobacterium-Mediated Transformation

Ejemplares similares