Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum

Abstract Neuromedin U (NmU) is a neuropeptide regulating diverse physiological processes. The insect homologs of vertebrate NmU are categorized as PRXamide family peptides due to their conserved C-terminal end. However, NmU homologs have been elusive in Mollusca, the second largest phylum in the ani...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Seung-Joon Ahn, Rory J. Mc Donnell, Jacob A. Corcoran, Ruth C. Martin, Man-Yeon Choi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/461b8a05f4054334b48d59b414948473
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:461b8a05f4054334b48d59b414948473
record_format	dspace
spelling	oai:doaj.org-article:461b8a05f4054334b48d59b4149484732021-12-02T13:58:15ZIdentification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum10.1038/s41598-020-79047-x2045-2322https://doaj.org/article/461b8a05f4054334b48d59b4149484732020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-79047-xhttps://doaj.org/toc/2045-2322Abstract Neuromedin U (NmU) is a neuropeptide regulating diverse physiological processes. The insect homologs of vertebrate NmU are categorized as PRXamide family peptides due to their conserved C-terminal end. However, NmU homologs have been elusive in Mollusca, the second largest phylum in the animal kingdom. Here we report the first molluscan NmU/PRXamide receptor from the slug, Deroceras reticulatum. Two splicing variants of the receptor gene were functionally expressed and tested for binding with ten endogenous peptides from the slug and some insect PRXamide and vertebrate NmU peptides. Three heptapeptides (QPPLPRYa, QPPVPRYa and AVPRPRIa) triggered significant activation of the receptors, suggesting that they are true ligands for the NmU/PRXamide receptor in the slug. Synthetic peptides with structural modifications at different amino acid positions provided important insights on the core moiety of the active peptides. One receptor variant always exhibited higher binding activity than the other variant. The NmU-encoding genes were highly expressed in the slug brain, while the receptor gene was expressed at lower levels in general with relatively higher expression levels in both the brain and foot. Injection of the bioactive peptides into slugs triggered defensive behavior such as copious mucus secretion and a range of other anomalous behaviors including immobilization, suggesting their role in important physiological functions.Seung-Joon AhnRory J. Mc DonnellJacob A. CorcoranRuth C. MartinMan-Yeon ChoiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-13 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seung-Joon Ahn Rory J. Mc Donnell Jacob A. Corcoran Ruth C. Martin Man-Yeon Choi Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
description	Abstract Neuromedin U (NmU) is a neuropeptide regulating diverse physiological processes. The insect homologs of vertebrate NmU are categorized as PRXamide family peptides due to their conserved C-terminal end. However, NmU homologs have been elusive in Mollusca, the second largest phylum in the animal kingdom. Here we report the first molluscan NmU/PRXamide receptor from the slug, Deroceras reticulatum. Two splicing variants of the receptor gene were functionally expressed and tested for binding with ten endogenous peptides from the slug and some insect PRXamide and vertebrate NmU peptides. Three heptapeptides (QPPLPRYa, QPPVPRYa and AVPRPRIa) triggered significant activation of the receptors, suggesting that they are true ligands for the NmU/PRXamide receptor in the slug. Synthetic peptides with structural modifications at different amino acid positions provided important insights on the core moiety of the active peptides. One receptor variant always exhibited higher binding activity than the other variant. The NmU-encoding genes were highly expressed in the slug brain, while the receptor gene was expressed at lower levels in general with relatively higher expression levels in both the brain and foot. Injection of the bioactive peptides into slugs triggered defensive behavior such as copious mucus secretion and a range of other anomalous behaviors including immobilization, suggesting their role in important physiological functions.
format	article
author	Seung-Joon Ahn Rory J. Mc Donnell Jacob A. Corcoran Ruth C. Martin Man-Yeon Choi
author_facet	Seung-Joon Ahn Rory J. Mc Donnell Jacob A. Corcoran Ruth C. Martin Man-Yeon Choi
author_sort	Seung-Joon Ahn
title	Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
title_short	Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
title_full	Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
title_fullStr	Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
title_full_unstemmed	Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum
title_sort	identification and functional characterization of the first molluscan neuromedin u receptor in the slug, deroceras reticulatum
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/461b8a05f4054334b48d59b414948473
work_keys_str_mv	AT seungjoonahn identificationandfunctionalcharacterizationofthefirstmolluscanneuromedinureceptorintheslugderocerasreticulatum AT roryjmcdonnell identificationandfunctionalcharacterizationofthefirstmolluscanneuromedinureceptorintheslugderocerasreticulatum AT jacobacorcoran identificationandfunctionalcharacterizationofthefirstmolluscanneuromedinureceptorintheslugderocerasreticulatum AT ruthcmartin identificationandfunctionalcharacterizationofthefirstmolluscanneuromedinureceptorintheslugderocerasreticulatum AT manyeonchoi identificationandfunctionalcharacterizationofthefirstmolluscanneuromedinureceptorintheslugderocerasreticulatum
_version_	1718392195553165312

Identification and functional characterization of the first molluscan neuromedin U receptor in the slug, Deroceras reticulatum

Ejemplares similares