Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway

Background. Propofol acts as an intravenous anesthetic cure which is widely used as a therapy for the craniocerebral injury that comprised surgical anesthesia as well as the sedation done in the intensive care units. Propofol is one of the most commonly used and efficient anesthetics where the painf...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Wen Li, Chenguang Qin, JianYong Yan, Qian Zhao, Lu You, Ye Yang
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Computer applications to medicine. Medical informatics R858-859.7
Acceso en línea:	https://doaj.org/article/461f0ff16f764c5d9b7908986ed94615
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:461f0ff16f764c5d9b7908986ed94615
record_format	dspace
spelling	oai:doaj.org-article:461f0ff16f764c5d9b7908986ed946152021-11-22T01:10:45ZPropofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway1748-671810.1155/2021/9305076https://doaj.org/article/461f0ff16f764c5d9b7908986ed946152021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/9305076https://doaj.org/toc/1748-6718Background. Propofol acts as an intravenous anesthetic cure which is widely used as a therapy for the craniocerebral injury that comprised surgical anesthesia as well as the sedation done in the intensive care units. Propofol is one of the most commonly used and efficient anesthetics where the painful effects are followed by an injection of propofol. In many cases, patients experience pain followed by anxiety, boredom, fear, and even myocardial ischemia. Objective. This study was performed to investigate the underlying mechanism of propofol and its effect on regulating spinal glun2b-p38mapkepac1 pathways in chronic contractile injury. Material and Methods. Contractile injury was performed by ligation around the nerve of the thigh region postanesthesia. Rats were divided into three groups to analyze the changes like mechanical allodynia by the paw withdrawal threshold and histopathological analysis for assessing cellular degradation. L4-L6 from the spinal dorsal horns were isolated and harvested for studying protein expression, by the method of western blotting and immunofluorescence analysis. Results. The pain caused due to mechanical allodynia in the paw region was highest at 1 hour postinduction and lasted for three days postinjury. Pain was significantly less in the group receiving propofol when compared with the isoflurane group for the first two hours of injury. In the propofol group, EPAC1, GluN2B, and p38 MAP K were significantly lower. Conclusion. In the rat model of induced chronic contractile injury, postsurgery there was a suppression of the GluN2B-p38MAPK/EPAC1 signaling pathway in the propofol group. As the p38MAPK/EPAC pathway has a significant role in the postoperative hyperalgesia, thus our experiment suggests that propofol has analgesic effects.Wen LiChenguang QinJianYong YanQian ZhaoLu YouYe YangHindawi LimitedarticleComputer applications to medicine. Medical informaticsR858-859.7ENComputational and Mathematical Methods in Medicine, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Computer applications to medicine. Medical informatics R858-859.7
spellingShingle	Computer applications to medicine. Medical informatics R858-859.7 Wen Li Chenguang Qin JianYong Yan Qian Zhao Lu You Ye Yang Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
description	Background. Propofol acts as an intravenous anesthetic cure which is widely used as a therapy for the craniocerebral injury that comprised surgical anesthesia as well as the sedation done in the intensive care units. Propofol is one of the most commonly used and efficient anesthetics where the painful effects are followed by an injection of propofol. In many cases, patients experience pain followed by anxiety, boredom, fear, and even myocardial ischemia. Objective. This study was performed to investigate the underlying mechanism of propofol and its effect on regulating spinal glun2b-p38mapkepac1 pathways in chronic contractile injury. Material and Methods. Contractile injury was performed by ligation around the nerve of the thigh region postanesthesia. Rats were divided into three groups to analyze the changes like mechanical allodynia by the paw withdrawal threshold and histopathological analysis for assessing cellular degradation. L4-L6 from the spinal dorsal horns were isolated and harvested for studying protein expression, by the method of western blotting and immunofluorescence analysis. Results. The pain caused due to mechanical allodynia in the paw region was highest at 1 hour postinduction and lasted for three days postinjury. Pain was significantly less in the group receiving propofol when compared with the isoflurane group for the first two hours of injury. In the propofol group, EPAC1, GluN2B, and p38 MAP K were significantly lower. Conclusion. In the rat model of induced chronic contractile injury, postsurgery there was a suppression of the GluN2B-p38MAPK/EPAC1 signaling pathway in the propofol group. As the p38MAPK/EPAC pathway has a significant role in the postoperative hyperalgesia, thus our experiment suggests that propofol has analgesic effects.
format	article
author	Wen Li Chenguang Qin JianYong Yan Qian Zhao Lu You Ye Yang
author_facet	Wen Li Chenguang Qin JianYong Yan Qian Zhao Lu You Ye Yang
author_sort	Wen Li
title	Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
title_short	Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
title_full	Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
title_fullStr	Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
title_full_unstemmed	Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway
title_sort	propofol alleviates neuropathic pain induced by chronic contractile injury by regulating the spinal glun2b-p38mapkepac1 pathway
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/461f0ff16f764c5d9b7908986ed94615
work_keys_str_mv	AT wenli propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway AT chenguangqin propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway AT jianyongyan propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway AT qianzhao propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway AT luyou propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway AT yeyang propofolalleviatesneuropathicpaininducedbychroniccontractileinjurybyregulatingthespinalglun2bp38mapkepac1pathway
_version_	1718418335525240832

Propofol Alleviates Neuropathic Pain Induced by Chronic Contractile Injury by Regulating the Spinal glun2b-p38mapkepac1 Pathway

Ejemplares similares