Genomic Comparison of Conjugative Plasmids from Salmonella enterica and Escherichia coli Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids

Salmonella enterica and Escherichia coli are important human pathogens that frequently contain plasmids, both large and small, carrying antibiotic resistance genes. Large conjugative plasmids are known to mobilize small Col plasmids, but less is known about the...

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Autores principales:	Elizabeth A. McMillan, Ly-Huong T. Nguyen, Lari M. Hiott, Poonam Sharma, Charlene R. Jackson, Jonathan G. Frye, Chin-Yi Chen
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	plasmids conjugation <i>Salmonella</i> <i>E. coli</i> Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/4624078301a44d2c8631a3331400c9c2
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spelling	oai:doaj.org-article:4624078301a44d2c8631a3331400c9c22021-11-25T18:24:14ZGenomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids10.3390/microorganisms91122052076-2607https://doaj.org/article/4624078301a44d2c8631a3331400c9c22021-10-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2205https://doaj.org/toc/2076-2607<i>Salmonella enterica</i> and <i>Escherichia coli</i> are important human pathogens that frequently contain plasmids, both large and small, carrying antibiotic resistance genes. Large conjugative plasmids are known to mobilize small Col plasmids, but less is known about the specificity of mobilization. In the current study, six <i>S. enterica</i> and four <i>E. coli</i> strains containing large plasmids were tested for their ability to mobilize three different kanamycin resistance Col plasmids (KanR plasmids). Large conjugative plasmids from five isolates, four <i>S. enterica</i> and one <i>E. coli</i>, were able to mobilize KanR plasmids of various types. Plasmids capable of mobilizing the KanR plasmids were either IncI1 or IncX, while IncI1 and IncX plasmids with no evidence of conjugation had disrupted transfer regions. Conjugative plasmids of similar types mobilized similar KanR plasmids, but not all conjugative plasmid types were capable of mobilizing all of the KanR plasmids. These data describe some of the complexities and specificities of individual small plasmid mobilization.Elizabeth A. McMillanLy-Huong T. NguyenLari M. HiottPoonam SharmaCharlene R. JacksonJonathan G. FryeChin-Yi ChenMDPI AGarticleplasmidsconjugation<i>Salmonella</i><i>E. coli</i>Biology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2205, p 2205 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	plasmids conjugation <i>Salmonella</i> <i>E. coli</i> Biology (General) QH301-705.5
spellingShingle	plasmids conjugation <i>Salmonella</i> <i>E. coli</i> Biology (General) QH301-705.5 Elizabeth A. McMillan Ly-Huong T. Nguyen Lari M. Hiott Poonam Sharma Charlene R. Jackson Jonathan G. Frye Chin-Yi Chen Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
description	<i>Salmonella enterica</i> and <i>Escherichia coli</i> are important human pathogens that frequently contain plasmids, both large and small, carrying antibiotic resistance genes. Large conjugative plasmids are known to mobilize small Col plasmids, but less is known about the specificity of mobilization. In the current study, six <i>S. enterica</i> and four <i>E. coli</i> strains containing large plasmids were tested for their ability to mobilize three different kanamycin resistance Col plasmids (KanR plasmids). Large conjugative plasmids from five isolates, four <i>S. enterica</i> and one <i>E. coli</i>, were able to mobilize KanR plasmids of various types. Plasmids capable of mobilizing the KanR plasmids were either IncI1 or IncX, while IncI1 and IncX plasmids with no evidence of conjugation had disrupted transfer regions. Conjugative plasmids of similar types mobilized similar KanR plasmids, but not all conjugative plasmid types were capable of mobilizing all of the KanR plasmids. These data describe some of the complexities and specificities of individual small plasmid mobilization.
format	article
author	Elizabeth A. McMillan Ly-Huong T. Nguyen Lari M. Hiott Poonam Sharma Charlene R. Jackson Jonathan G. Frye Chin-Yi Chen
author_facet	Elizabeth A. McMillan Ly-Huong T. Nguyen Lari M. Hiott Poonam Sharma Charlene R. Jackson Jonathan G. Frye Chin-Yi Chen
author_sort	Elizabeth A. McMillan
title	Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
title_short	Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
title_full	Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
title_fullStr	Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
title_full_unstemmed	Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids
title_sort	genomic comparison of conjugative plasmids from <i>salmonella enterica</i> and <i>escherichia coli</i> encoding beta-lactamases and capable of mobilizing kanamycin resistance col-like plasmids
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/4624078301a44d2c8631a3331400c9c2
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Genomic Comparison of Conjugative Plasmids from <i>Salmonella enterica</i> and <i>Escherichia coli</i> Encoding Beta-Lactamases and Capable of Mobilizing Kanamycin Resistance Col-like Plasmids

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