The gastric microbiota in patients with Crohn’s disease; a preliminary study
Abstract The gastric microbiota in Crohn’s disease (CD) has not been studied. The purpose of the study was to evaluate differences of stomach microbiota between CD patients and controls. DNA was extracted from gastric mucosal and fluid samples, from 24 CD patients and 19 controls. 16S rRNA gene sequ...
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Nature Portfolio
2021
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oai:doaj.org-article:4624e7288dc040a8a91f105a486938612021-12-02T19:12:31ZThe gastric microbiota in patients with Crohn’s disease; a preliminary study10.1038/s41598-021-97261-z2045-2322https://doaj.org/article/4624e7288dc040a8a91f105a486938612021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97261-zhttps://doaj.org/toc/2045-2322Abstract The gastric microbiota in Crohn’s disease (CD) has not been studied. The purpose of the study was to evaluate differences of stomach microbiota between CD patients and controls. DNA was extracted from gastric mucosal and fluid samples, from 24 CD patients and 19 controls. 16S rRNA gene sequencing identified 1511 operational taxonomic units (OTUs), of which 239 passed the low abundance and low variance filters. All but one CD patients were HP negative. Fifteen bacterial phyla were identified in at least one mucosal or fluid site. Of these, Bacteroidota and Firmicutes accounted for 70% of all phyla. Proteobacteria, Actinobacteriota, and Fusobacteriota combined accounted for 27%. There was significant difference in the relative abundance of Bacteroidota, Proteobacteria, Fusobacteriota, and Campilobacterota between CD patients and controls only in gastric corpus samples. In gastric liquid, there was a significant difference only in Actinobacteriota. Pairwise comparison identified 67 differentially abundant OTUs in at least one site. Of these, 13 were present in more than one comparison, and four differentiating OTUs (Neisseriaceae, Neisseria, Absconditabacteriales, and Microbacteriaceae) were identified at all tested sites. The results reveal significant changes in gastric microbial profiles (beta diversity, phylum, and individual taxa levels) between H. pylori-negative CD patients and controls.Jerzy OstrowskiMaria KuleckaIwona ZawadaNatalia Żeber-LubeckaAgnieszka PaziewskaKatarzyna Graca-PakulskaKrzysztof DąbkowskiKarolina SkubiszPatrycja CybulaFilip AmbrożkiewiczElżbieta UrasińskaMichał MikulaTeresa StarzyńskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021) |
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Medicine R Science Q Jerzy Ostrowski Maria Kulecka Iwona Zawada Natalia Żeber-Lubecka Agnieszka Paziewska Katarzyna Graca-Pakulska Krzysztof Dąbkowski Karolina Skubisz Patrycja Cybula Filip Ambrożkiewicz Elżbieta Urasińska Michał Mikula Teresa Starzyńska The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| description |
Abstract The gastric microbiota in Crohn’s disease (CD) has not been studied. The purpose of the study was to evaluate differences of stomach microbiota between CD patients and controls. DNA was extracted from gastric mucosal and fluid samples, from 24 CD patients and 19 controls. 16S rRNA gene sequencing identified 1511 operational taxonomic units (OTUs), of which 239 passed the low abundance and low variance filters. All but one CD patients were HP negative. Fifteen bacterial phyla were identified in at least one mucosal or fluid site. Of these, Bacteroidota and Firmicutes accounted for 70% of all phyla. Proteobacteria, Actinobacteriota, and Fusobacteriota combined accounted for 27%. There was significant difference in the relative abundance of Bacteroidota, Proteobacteria, Fusobacteriota, and Campilobacterota between CD patients and controls only in gastric corpus samples. In gastric liquid, there was a significant difference only in Actinobacteriota. Pairwise comparison identified 67 differentially abundant OTUs in at least one site. Of these, 13 were present in more than one comparison, and four differentiating OTUs (Neisseriaceae, Neisseria, Absconditabacteriales, and Microbacteriaceae) were identified at all tested sites. The results reveal significant changes in gastric microbial profiles (beta diversity, phylum, and individual taxa levels) between H. pylori-negative CD patients and controls. |
| format |
article |
| author |
Jerzy Ostrowski Maria Kulecka Iwona Zawada Natalia Żeber-Lubecka Agnieszka Paziewska Katarzyna Graca-Pakulska Krzysztof Dąbkowski Karolina Skubisz Patrycja Cybula Filip Ambrożkiewicz Elżbieta Urasińska Michał Mikula Teresa Starzyńska |
| author_facet |
Jerzy Ostrowski Maria Kulecka Iwona Zawada Natalia Żeber-Lubecka Agnieszka Paziewska Katarzyna Graca-Pakulska Krzysztof Dąbkowski Karolina Skubisz Patrycja Cybula Filip Ambrożkiewicz Elżbieta Urasińska Michał Mikula Teresa Starzyńska |
| author_sort |
Jerzy Ostrowski |
| title |
The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| title_short |
The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| title_full |
The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| title_fullStr |
The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| title_full_unstemmed |
The gastric microbiota in patients with Crohn’s disease; a preliminary study |
| title_sort |
gastric microbiota in patients with crohn’s disease; a preliminary study |
| publisher |
Nature Portfolio |
| publishDate |
2021 |
| url |
https://doaj.org/article/4624e7288dc040a8a91f105a48693861 |
| work_keys_str_mv |
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