Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis

Abstract During sporulation Bacillus subtilis Mfd couples transcription to nucleotide excision repair (NER) to eliminate DNA distorting lesions. Here, we report a significant decline in sporulation following Mfd disruption, which was manifested in the absence of external DNA-damage suggesting that s...

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Autores principales:	Valeria P. Suárez, Lissett E. Martínez, Hilda C. Leyva-Sánchez, Luz I. Valenzuela-García, Reyna Lara-Martínez, Luis F. Jiménez-García, Norma Ramírez-Ramírez, Armando Obregon-Herrera, Mayra Cuéllar-Cruz, Eduardo A. Robleto, Mario Pedraza-Reyes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/462db530877b45fe9abba92899a12328
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spelling	oai:doaj.org-article:462db530877b45fe9abba92899a123282021-12-02T14:16:07ZTranscriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis10.1038/s41598-021-82247-82045-2322https://doaj.org/article/462db530877b45fe9abba92899a123282021-01-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-82247-8https://doaj.org/toc/2045-2322Abstract During sporulation Bacillus subtilis Mfd couples transcription to nucleotide excision repair (NER) to eliminate DNA distorting lesions. Here, we report a significant decline in sporulation following Mfd disruption, which was manifested in the absence of external DNA-damage suggesting that spontaneous lesions activate the function of Mfd for an efficient sporogenesis. Accordingly, a dramatic decline in sporulation efficiency took place in a B. subtilis strain lacking Mfd and the repair/prevention guanine oxidized (GO) system (hereafter, the ∆GO system), composed by YtkD, MutM and MutY. Furthermore, the simultaneous absence of Mfd and the GO system, (i) sensitized sporulating cells to H2O2, and (ii) elicited spontaneous and oxygen radical-induced rifampin-resistance (Rifr) mutagenesis. Epifluorescence (EF), confocal and transmission electron (TEM) microscopy analyses, showed a decreased ability of ∆GO ∆mfd strain to sporulate and to develop the typical morphologies of sporulating cells. Remarkably, disruption of sda, sirA and disA partially, restored the sporulation efficiency of the strain deficient for Mfd and the ∆GO system; complete restoration occurred in the RecA− background. Overall, our results unveil a novel Mfd mechanism of transcription-coupled-repair (TCR) elicited by 8-OxoG which converges in the activation of a RecA-dependent checkpoint event that control the onset of sporulation in B. subtilis.Valeria P. SuárezLissett E. MartínezHilda C. Leyva-SánchezLuz I. Valenzuela-GarcíaReyna Lara-MartínezLuis F. Jiménez-GarcíaNorma Ramírez-RamírezArmando Obregon-HerreraMayra Cuéllar-CruzEduardo A. RobletoMario Pedraza-ReyesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Valeria P. Suárez Lissett E. Martínez Hilda C. Leyva-Sánchez Luz I. Valenzuela-García Reyna Lara-Martínez Luis F. Jiménez-García Norma Ramírez-Ramírez Armando Obregon-Herrera Mayra Cuéllar-Cruz Eduardo A. Robleto Mario Pedraza-Reyes Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
description	Abstract During sporulation Bacillus subtilis Mfd couples transcription to nucleotide excision repair (NER) to eliminate DNA distorting lesions. Here, we report a significant decline in sporulation following Mfd disruption, which was manifested in the absence of external DNA-damage suggesting that spontaneous lesions activate the function of Mfd for an efficient sporogenesis. Accordingly, a dramatic decline in sporulation efficiency took place in a B. subtilis strain lacking Mfd and the repair/prevention guanine oxidized (GO) system (hereafter, the ∆GO system), composed by YtkD, MutM and MutY. Furthermore, the simultaneous absence of Mfd and the GO system, (i) sensitized sporulating cells to H2O2, and (ii) elicited spontaneous and oxygen radical-induced rifampin-resistance (Rifr) mutagenesis. Epifluorescence (EF), confocal and transmission electron (TEM) microscopy analyses, showed a decreased ability of ∆GO ∆mfd strain to sporulate and to develop the typical morphologies of sporulating cells. Remarkably, disruption of sda, sirA and disA partially, restored the sporulation efficiency of the strain deficient for Mfd and the ∆GO system; complete restoration occurred in the RecA− background. Overall, our results unveil a novel Mfd mechanism of transcription-coupled-repair (TCR) elicited by 8-OxoG which converges in the activation of a RecA-dependent checkpoint event that control the onset of sporulation in B. subtilis.
format	article
author	Valeria P. Suárez Lissett E. Martínez Hilda C. Leyva-Sánchez Luz I. Valenzuela-García Reyna Lara-Martínez Luis F. Jiménez-García Norma Ramírez-Ramírez Armando Obregon-Herrera Mayra Cuéllar-Cruz Eduardo A. Robleto Mario Pedraza-Reyes
author_facet	Valeria P. Suárez Lissett E. Martínez Hilda C. Leyva-Sánchez Luz I. Valenzuela-García Reyna Lara-Martínez Luis F. Jiménez-García Norma Ramírez-Ramírez Armando Obregon-Herrera Mayra Cuéllar-Cruz Eduardo A. Robleto Mario Pedraza-Reyes
author_sort	Valeria P. Suárez
title	Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
title_short	Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
title_full	Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
title_fullStr	Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
title_full_unstemmed	Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis
title_sort	transcriptional coupling and repair of 8-oxog activate a reca-dependent checkpoint that controls the onset of sporulation in bacillus subtilis
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/462db530877b45fe9abba92899a12328
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Transcriptional coupling and repair of 8-OxoG activate a RecA-dependent checkpoint that controls the onset of sporulation in Bacillus subtilis

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