Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey

Tick-borne diseases, such as those transmitted by the blacklegged tick Ixodes scapularis, are a significant and growing public health problem in the US. There is mounting evidence that co-occurring non-pathogenic microbes can also impact tick-borne disease transmission. Shotgun metagenome sequencing...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Dana C. Price, Julia R. Brennan, Nicole E. Wagner, Andrea M. Egizi
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Blacklegged tick Ixodes scapularis Deer tick Rickettsia buchneri Hologenomics Metagenomics Medicine R
Acceso en línea:	https://doaj.org/article/462ec1cc71624f90bb2e58362eab5da7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:462ec1cc71624f90bb2e58362eab5da7
record_format	dspace
spelling	oai:doaj.org-article:462ec1cc71624f90bb2e58362eab5da72021-11-11T15:05:24ZComparative hologenomics of two Ixodes scapularis tick populations in New Jersey10.7717/peerj.123132167-8359https://doaj.org/article/462ec1cc71624f90bb2e58362eab5da72021-11-01T00:00:00Zhttps://peerj.com/articles/12313.pdfhttps://peerj.com/articles/12313/https://doaj.org/toc/2167-8359Tick-borne diseases, such as those transmitted by the blacklegged tick Ixodes scapularis, are a significant and growing public health problem in the US. There is mounting evidence that co-occurring non-pathogenic microbes can also impact tick-borne disease transmission. Shotgun metagenome sequencing enables sampling of the complete tick hologenome—the collective genomes of the tick and all of the microbial species contained therein, whether pathogenic, commensal or symbiotic. This approach simultaneously uncovers taxonomic composition and allows the detection of intraspecific genetic variation, making it a useful tool to compare spatial differences across tick populations. We evaluated this approach by comparing hologenome data from two tick samples (N = 6 ticks per location) collected at a relatively fine spatial scale, approximately 23 km apart, within a single US county. Several intriguing variants in the data between the two sites were detected, including polymorphisms in both in the tick’s own mitochondrial DNA and that of a rickettsial endosymbiont. The two samples were broadly similar in terms of the microbial species present, including multiple known tick-borne pathogens (Borrelia burgdorferi, Babesia microti, and Anaplasma phagocytophilum), filarial nematodes, and Wolbachia and Babesia species. We assembled the complete genome of the rickettsial endosymbiont (most likely Rickettsia buchneri) from both populations. Our results provide further evidence for the use of shotgun metagenome sequencing as a tool to compare tick hologenomes and differentiate tick populations across localized spatial scales.Dana C. PriceJulia R. BrennanNicole E. WagnerAndrea M. EgiziPeerJ Inc.articleBlacklegged tickIxodes scapularisDeer tickRickettsia buchneriHologenomicsMetagenomicsMedicineRENPeerJ, Vol 9, p e12313 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Blacklegged tick Ixodes scapularis Deer tick Rickettsia buchneri Hologenomics Metagenomics Medicine R
spellingShingle	Blacklegged tick Ixodes scapularis Deer tick Rickettsia buchneri Hologenomics Metagenomics Medicine R Dana C. Price Julia R. Brennan Nicole E. Wagner Andrea M. Egizi Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
description	Tick-borne diseases, such as those transmitted by the blacklegged tick Ixodes scapularis, are a significant and growing public health problem in the US. There is mounting evidence that co-occurring non-pathogenic microbes can also impact tick-borne disease transmission. Shotgun metagenome sequencing enables sampling of the complete tick hologenome—the collective genomes of the tick and all of the microbial species contained therein, whether pathogenic, commensal or symbiotic. This approach simultaneously uncovers taxonomic composition and allows the detection of intraspecific genetic variation, making it a useful tool to compare spatial differences across tick populations. We evaluated this approach by comparing hologenome data from two tick samples (N = 6 ticks per location) collected at a relatively fine spatial scale, approximately 23 km apart, within a single US county. Several intriguing variants in the data between the two sites were detected, including polymorphisms in both in the tick’s own mitochondrial DNA and that of a rickettsial endosymbiont. The two samples were broadly similar in terms of the microbial species present, including multiple known tick-borne pathogens (Borrelia burgdorferi, Babesia microti, and Anaplasma phagocytophilum), filarial nematodes, and Wolbachia and Babesia species. We assembled the complete genome of the rickettsial endosymbiont (most likely Rickettsia buchneri) from both populations. Our results provide further evidence for the use of shotgun metagenome sequencing as a tool to compare tick hologenomes and differentiate tick populations across localized spatial scales.
format	article
author	Dana C. Price Julia R. Brennan Nicole E. Wagner Andrea M. Egizi
author_facet	Dana C. Price Julia R. Brennan Nicole E. Wagner Andrea M. Egizi
author_sort	Dana C. Price
title	Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
title_short	Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
title_full	Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
title_fullStr	Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
title_full_unstemmed	Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey
title_sort	comparative hologenomics of two ixodes scapularis tick populations in new jersey
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/462ec1cc71624f90bb2e58362eab5da7
work_keys_str_mv	AT danacprice comparativehologenomicsoftwoixodesscapularistickpopulationsinnewjersey AT juliarbrennan comparativehologenomicsoftwoixodesscapularistickpopulationsinnewjersey AT nicoleewagner comparativehologenomicsoftwoixodesscapularistickpopulationsinnewjersey AT andreamegizi comparativehologenomicsoftwoixodesscapularistickpopulationsinnewjersey
_version_	1718437128674738176

Comparative hologenomics of two Ixodes scapularis tick populations in New Jersey

Ejemplares similares