Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action

Large-scale screens of chemical and genetic vulnerabilities in cancer are typically limited to simple readouts of cell viability. Here, the authors develop a method for profiling post-perturbation transcriptional responses across large pools of cancer cell lines, enabling deep characterization of sh...

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Autores principales:	James M. McFarland, Brenton R. Paolella, Allison Warren, Kathryn Geiger-Schuller, Tsukasa Shibue, Michael Rothberg, Olena Kuksenko, William N. Colgan, Andrew Jones, Emily Chambers, Danielle Dionne, Samantha Bender, Brian M. Wolpin, Mahmoud Ghandi, Itay Tirosh, Orit Rozenblatt-Rosen, Jennifer A. Roth, Todd R. Golub, Aviv Regev, Andrew J. Aguirre, Francisca Vazquez, Aviad Tsherniak
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/464481c452404d5080918310ac391162
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spelling	oai:doaj.org-article:464481c452404d5080918310ac3911622021-12-02T18:53:20ZMultiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action10.1038/s41467-020-17440-w2041-1723https://doaj.org/article/464481c452404d5080918310ac3911622020-08-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-17440-whttps://doaj.org/toc/2041-1723Large-scale screens of chemical and genetic vulnerabilities in cancer are typically limited to simple readouts of cell viability. Here, the authors develop a method for profiling post-perturbation transcriptional responses across large pools of cancer cell lines, enabling deep characterization of shared and context-specific responses.James M. McFarlandBrenton R. PaolellaAllison WarrenKathryn Geiger-SchullerTsukasa ShibueMichael RothbergOlena KuksenkoWilliam N. ColganAndrew JonesEmily ChambersDanielle DionneSamantha BenderBrian M. WolpinMahmoud GhandiItay TiroshOrit Rozenblatt-RosenJennifer A. RothTodd R. GolubAviv RegevAndrew J. AguirreFrancisca VazquezAviad TsherniakNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-15 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q James M. McFarland Brenton R. Paolella Allison Warren Kathryn Geiger-Schuller Tsukasa Shibue Michael Rothberg Olena Kuksenko William N. Colgan Andrew Jones Emily Chambers Danielle Dionne Samantha Bender Brian M. Wolpin Mahmoud Ghandi Itay Tirosh Orit Rozenblatt-Rosen Jennifer A. Roth Todd R. Golub Aviv Regev Andrew J. Aguirre Francisca Vazquez Aviad Tsherniak Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
description	Large-scale screens of chemical and genetic vulnerabilities in cancer are typically limited to simple readouts of cell viability. Here, the authors develop a method for profiling post-perturbation transcriptional responses across large pools of cancer cell lines, enabling deep characterization of shared and context-specific responses.
format	article
author	James M. McFarland Brenton R. Paolella Allison Warren Kathryn Geiger-Schuller Tsukasa Shibue Michael Rothberg Olena Kuksenko William N. Colgan Andrew Jones Emily Chambers Danielle Dionne Samantha Bender Brian M. Wolpin Mahmoud Ghandi Itay Tirosh Orit Rozenblatt-Rosen Jennifer A. Roth Todd R. Golub Aviv Regev Andrew J. Aguirre Francisca Vazquez Aviad Tsherniak
author_facet	James M. McFarland Brenton R. Paolella Allison Warren Kathryn Geiger-Schuller Tsukasa Shibue Michael Rothberg Olena Kuksenko William N. Colgan Andrew Jones Emily Chambers Danielle Dionne Samantha Bender Brian M. Wolpin Mahmoud Ghandi Itay Tirosh Orit Rozenblatt-Rosen Jennifer A. Roth Todd R. Golub Aviv Regev Andrew J. Aguirre Francisca Vazquez Aviad Tsherniak
author_sort	James M. McFarland
title	Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
title_short	Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
title_full	Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
title_fullStr	Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
title_full_unstemmed	Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
title_sort	multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/464481c452404d5080918310ac391162
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Multiplexed single-cell transcriptional response profiling to define cancer vulnerabilities and therapeutic mechanism of action

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