Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method

Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including...

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Autores principales:	HARIYANTI HARIYANTI, HANIFAH RAHMI
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Syiah Kuala 2021
Materias:	ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/465aa35384c8477fb61874dba2003c56
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spelling	oai:doaj.org-article:465aa35384c8477fb61874dba2003c562021-11-18T07:51:52ZIdentification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method1411-85132541-406210.24815/jn.v21i3.18874https://doaj.org/article/465aa35384c8477fb61874dba2003c562021-10-01T00:00:00Zhttp://jurnal.unsyiah.ac.id/natural/article/view/18874https://doaj.org/toc/1411-8513https://doaj.org/toc/2541-4062Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}HARIYANTI HARIYANTIHANIFAH RAHMIUniversitas Syiah Kualaarticleammonium sulphate, amylase, size exclusion chromatography, vannamei shrimpScience (General)Q1-390ENJurnal Natural, Vol 21, Iss 3, Pp 123-127 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp Science (General) Q1-390
spellingShingle	ammonium sulphate, amylase, size exclusion chromatography, vannamei shrimp Science (General) Q1-390 HARIYANTI HARIYANTI HANIFAH RAHMI Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
description	Vannamei shrimps (Litopenaeus vannamei), also known as white leg shrimps are widely cultivated and consumed by many people. However, most consumers have removed the shrimp’s head which integrates with its digestive tract. The digestive tract of white leg shrimp contains digestive enzymes, including amylase. This study aimed to determine the protein content and amylase activity from Vannamei shrimps’ digestive tract using the size exclusion chromatography method. The protein isolation of size exclusion chromatography was prepared in three steps, namely; centrifugation, precipitation, and dialysis. The protein from the dialysis step was purified by using gel filtration chromatography. Each obtained fraction was determined the protein content and amylase activity by using spectrophotometer UV-Vis method. The results showed that the fraction of 108th had the highest protein content and amylase activity with a value of 0.85 mg/ml and 25.66U/ml respectively. @font-face {font-family:"Cambria Math"; panose-1:2 4 5 3 5 4 6 3 2 4; mso-font-charset:0; mso-generic-font-family:roman; mso-font-pitch:variable; mso-font-signature:-536869121 1107305727 33554432 0 415 0;}p.MsoNormal, li.MsoNormal, div.MsoNormal {mso-style-unhide:no; mso-style-qformat:yes; mso-style-parent:""; margin:0cm; mso-pagination:widow-orphan; font-size:12.0pt; font-family:"Times New Roman",serif; mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-US;}.MsoChpDefault {mso-style-type:export-only; mso-default-props:yes; font-size:10.0pt; mso-ansi-font-size:10.0pt; mso-bidi-font-size:10.0pt; mso-ansi-language:IN; mso-fareast-language:IN;}div.WordSection1 {page:WordSection1;}
format	article
author	HARIYANTI HARIYANTI HANIFAH RAHMI
author_facet	HARIYANTI HARIYANTI HANIFAH RAHMI
author_sort	HARIYANTI HARIYANTI
title	Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
title_short	Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
title_full	Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
title_fullStr	Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
title_full_unstemmed	Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method
title_sort	identification of amylase activity from vannamei shrimps’ (litopenaeus vannamei) digestive tract using size exclusion chromatography method
publisher	Universitas Syiah Kuala
publishDate	2021
url	https://doaj.org/article/465aa35384c8477fb61874dba2003c56
work_keys_str_mv	AT hariyantihariyanti identificationofamylaseactivityfromvannameishrimpslitopenaeusvannameidigestivetractusingsizeexclusionchromatographymethod AT hanifahrahmi identificationofamylaseactivityfromvannameishrimpslitopenaeusvannameidigestivetractusingsizeexclusionchromatographymethod
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Identification of amylase activity from vannamei shrimps’ (Litopenaeus vannamei) digestive tract using size exclusion chromatography method

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