Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)

Abstract. Sari DN, Nasrullah H, Ekasari J, Suprayudi MA, Alimuddin A. 2021. Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy). Biodiversitas 22: 741-750. Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolis...

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Autores principales:	Dian Novita Sari, Hasan Nasrullah, Julie Ekasari, Muhammad Agus Suprayudi, Alimuddin Alimuddin
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	giant gourami, glucokinase, gene expression, herbivorous, osphronemus gouramy Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/465f092a16d844a8951d5889c64ae6f5
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spelling	oai:doaj.org-article:465f092a16d844a8951d5889c64ae6f52021-11-22T00:54:56ZSequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)1412-033X2085-472210.13057/biodiv/d220227https://doaj.org/article/465f092a16d844a8951d5889c64ae6f52021-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/7266https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Sari DN, Nasrullah H, Ekasari J, Suprayudi MA, Alimuddin A. 2021. Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy). Biodiversitas 22: 741-750. Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolism and high glucose homeostatic in fish. The information about the GCK mRNA sequence and its expression is limited in Giant gourami, one of the most important herbivorous aquaculture species in Indonesia. The present study aimed to characterize the GCK mRNA and analyze its mRNA expression and plasma glucose levels after high glucose injection in Giant gourami. We also compared its sequence variability among carnivorous and herbivorous fish. The GCK mRNA was identified using polymerase chain reaction (PCR) method from the fish liver. Its mRNA level was analyzed by real-time PCR (qPCR). Giant gourami GCK mRNA sequence was 2104 nucleotide long, encoding 478 amino acids, and shared high similarity with other fish. GCK was mainly expressed in the liver. The mRNA level of GCK was highly up-regulated after 6 hours of high glucose injection, in-line with the plasma glucose in the blood. There are no major differences observed in the GCK amino acid sequences among Giant gourami and other fish. The knowledge gained from this study could be used as a reference for further exploration of metabolic regulation in Giant gourami.Dian Novita SariHasan NasrullahJulie EkasariMuhammad Agus SuprayudiAlimuddin AlimuddinMBI & UNS Soloarticlegiant gourami, glucokinase, gene expression, herbivorous, osphronemus gouramyBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	giant gourami, glucokinase, gene expression, herbivorous, osphronemus gouramy Biology (General) QH301-705.5
spellingShingle	giant gourami, glucokinase, gene expression, herbivorous, osphronemus gouramy Biology (General) QH301-705.5 Dian Novita Sari Hasan Nasrullah Julie Ekasari Muhammad Agus Suprayudi Alimuddin Alimuddin Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
description	Abstract. Sari DN, Nasrullah H, Ekasari J, Suprayudi MA, Alimuddin A. 2021. Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy). Biodiversitas 22: 741-750. Glucokinase (GCK) is one of the enzymes that play important roles in carbohydrate metabolism and high glucose homeostatic in fish. The information about the GCK mRNA sequence and its expression is limited in Giant gourami, one of the most important herbivorous aquaculture species in Indonesia. The present study aimed to characterize the GCK mRNA and analyze its mRNA expression and plasma glucose levels after high glucose injection in Giant gourami. We also compared its sequence variability among carnivorous and herbivorous fish. The GCK mRNA was identified using polymerase chain reaction (PCR) method from the fish liver. Its mRNA level was analyzed by real-time PCR (qPCR). Giant gourami GCK mRNA sequence was 2104 nucleotide long, encoding 478 amino acids, and shared high similarity with other fish. GCK was mainly expressed in the liver. The mRNA level of GCK was highly up-regulated after 6 hours of high glucose injection, in-line with the plasma glucose in the blood. There are no major differences observed in the GCK amino acid sequences among Giant gourami and other fish. The knowledge gained from this study could be used as a reference for further exploration of metabolic regulation in Giant gourami.
format	article
author	Dian Novita Sari Hasan Nasrullah Julie Ekasari Muhammad Agus Suprayudi Alimuddin Alimuddin
author_facet	Dian Novita Sari Hasan Nasrullah Julie Ekasari Muhammad Agus Suprayudi Alimuddin Alimuddin
author_sort	Dian Novita Sari
title	Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
title_short	Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
title_full	Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
title_fullStr	Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
title_full_unstemmed	Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)
title_sort	sequence and expression analysis of glucokinase mrna from herbivorous giant gourami (osphronemus goramy)
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/465f092a16d844a8951d5889c64ae6f5
work_keys_str_mv	AT diannovitasari sequenceandexpressionanalysisofglucokinasemrnafromherbivorousgiantgouramiosphronemusgoramy AT hasannasrullah sequenceandexpressionanalysisofglucokinasemrnafromherbivorousgiantgouramiosphronemusgoramy AT julieekasari sequenceandexpressionanalysisofglucokinasemrnafromherbivorousgiantgouramiosphronemusgoramy AT muhammadagussuprayudi sequenceandexpressionanalysisofglucokinasemrnafromherbivorousgiantgouramiosphronemusgoramy AT alimuddinalimuddin sequenceandexpressionanalysisofglucokinasemrnafromherbivorousgiantgouramiosphronemusgoramy
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Sequence and expression analysis of glucokinase mRNA from herbivorous Giant gourami (Osphronemus goramy)

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