Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period

Background. Violation of the regeneration of the tracheal wall after undergoing reconstructive surgeries remains an urgent problem of modern surgery. One of the reasons for the violation of the regeneration processes is insufficient blood supply to the organ. Finding new ways to improve blood flow i...

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Autores principales:	I. S. Kurganskiy, S. A. Lepekhova, O. A. Goldberg, L. V. Korobeynikova, E. O. Inozemtsev, Yu. I. Pivovarov, E. V. Grigoryev
Formato:	article
Lenguaje:	RU
Publicado:	Scientific Сentre for Family Health and Human Reproduction Problems 2021
Materias:	trachea regeneration vegf experiment rat Science Q
Acceso en línea:	https://doaj.org/article/466113a26a0f4898876b39378b78579c
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spelling	oai:doaj.org-article:466113a26a0f4898876b39378b78579c2021-11-23T06:14:45ZEvaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period2541-94202587-959610.29413/ABS.2020-5.6.37https://doaj.org/article/466113a26a0f4898876b39378b78579c2021-01-01T00:00:00Zhttps://www.actabiomedica.ru/jour/article/view/2529https://doaj.org/toc/2541-9420https://doaj.org/toc/2587-9596Background. Violation of the regeneration of the tracheal wall after undergoing reconstructive surgeries remains an urgent problem of modern surgery. One of the reasons for the violation of the regeneration processes is insufficient blood supply to the organ. Finding new ways to improve blood flow is a promising area of research.Aim. To evaluate the effectiveness of VEGF effect on the regeneration of the anterior tracheal surface in the early postoperative period.Materials and methods. The study included 60 male Wistar rats at the age of 10 months. All animals underwent tracheal reconstructive surgery; the animals were divided into three groups: group 1 – without medicinal film, group 2 – with prolonged-release medicinal film, group 3 – with prolonged-release medicinal film containing VEGF. The sampling of histological material was carried out on days 3, 7 and 21 of the study, using morphometry to determine the thickness of the tracheal wall, the thickness of the postoperative scar, the area of the surgical intervention zone, the area of the vessels, the ratio of the thickness of the postoperative scar to the wall thickness trachea and the ratio of the area of the vessels to the area of the surgical intervention zone.Results. It was revealed that when exposed to a long-acting medicinal film containing VEGF, significant histological changes occurred on day 7 of the study. In groups 1 and 2, healing occurred by day 21 and was accompanied by defects of the mucous membrane and cartilage. On day 7 in group 3, there was a complete epithelialization of the tracheal wall, an increase in the number of newly formed vessels and glands in the area of surgical intervention. Healing was carried out with a thin postoperative scar without defects in all layers of the tracheal wall.Conclusion. The results obtained indicate the stimulating effect of VEGF on the regeneration of the tracheal wall in the early postoperative period after undergoing reconstructive surgery. The use of VEGF reduces the time of postoperative wound healing and preserves the anatomical structure of the tracheal wall.I. S. KurganskiyS. A. LepekhovaO. A. GoldbergL. V. KorobeynikovaE. O. InozemtsevYu. I. PivovarovE. V. GrigoryevScientific Сentre for Family Health and Human Reproduction ProblemsarticletrachearegenerationvegfexperimentratScienceQRUActa Biomedica Scientifica, Vol 5, Iss 6, Pp 276-281 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	trachea regeneration vegf experiment rat Science Q
spellingShingle	trachea regeneration vegf experiment rat Science Q I. S. Kurganskiy S. A. Lepekhova O. A. Goldberg L. V. Korobeynikova E. O. Inozemtsev Yu. I. Pivovarov E. V. Grigoryev Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
description	Background. Violation of the regeneration of the tracheal wall after undergoing reconstructive surgeries remains an urgent problem of modern surgery. One of the reasons for the violation of the regeneration processes is insufficient blood supply to the organ. Finding new ways to improve blood flow is a promising area of research.Aim. To evaluate the effectiveness of VEGF effect on the regeneration of the anterior tracheal surface in the early postoperative period.Materials and methods. The study included 60 male Wistar rats at the age of 10 months. All animals underwent tracheal reconstructive surgery; the animals were divided into three groups: group 1 – without medicinal film, group 2 – with prolonged-release medicinal film, group 3 – with prolonged-release medicinal film containing VEGF. The sampling of histological material was carried out on days 3, 7 and 21 of the study, using morphometry to determine the thickness of the tracheal wall, the thickness of the postoperative scar, the area of the surgical intervention zone, the area of the vessels, the ratio of the thickness of the postoperative scar to the wall thickness trachea and the ratio of the area of the vessels to the area of the surgical intervention zone.Results. It was revealed that when exposed to a long-acting medicinal film containing VEGF, significant histological changes occurred on day 7 of the study. In groups 1 and 2, healing occurred by day 21 and was accompanied by defects of the mucous membrane and cartilage. On day 7 in group 3, there was a complete epithelialization of the tracheal wall, an increase in the number of newly formed vessels and glands in the area of surgical intervention. Healing was carried out with a thin postoperative scar without defects in all layers of the tracheal wall.Conclusion. The results obtained indicate the stimulating effect of VEGF on the regeneration of the tracheal wall in the early postoperative period after undergoing reconstructive surgery. The use of VEGF reduces the time of postoperative wound healing and preserves the anatomical structure of the tracheal wall.
format	article
author	I. S. Kurganskiy S. A. Lepekhova O. A. Goldberg L. V. Korobeynikova E. O. Inozemtsev Yu. I. Pivovarov E. V. Grigoryev
author_facet	I. S. Kurganskiy S. A. Lepekhova O. A. Goldberg L. V. Korobeynikova E. O. Inozemtsev Yu. I. Pivovarov E. V. Grigoryev
author_sort	I. S. Kurganskiy
title	Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
title_short	Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
title_full	Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
title_fullStr	Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
title_full_unstemmed	Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period
title_sort	evaluation of the effectiveness of vegf effect on the regeneration of the anterior tracheal surface in the early postoperative period
publisher	Scientific Сentre for Family Health and Human Reproduction Problems
publishDate	2021
url	https://doaj.org/article/466113a26a0f4898876b39378b78579c
work_keys_str_mv	AT iskurganskiy evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT salepekhova evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT oagoldberg evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT lvkorobeynikova evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT eoinozemtsev evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT yuipivovarov evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod AT evgrigoryev evaluationoftheeffectivenessofvegfeffectontheregenerationoftheanteriortrachealsurfaceintheearlypostoperativeperiod
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Evaluation of the Effectiveness of VEGF Effect on the Regeneration of the Anterior Tracheal Surface in the Early Postoperative Period

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