Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study

Christine W Sindt1, Trudy K Grout1, D Brice Critser1, Jami R Kern2, David L Meadows21University of Iowa Hospitals and Clinics, Iowa City, IA; 2Alcon Research Ltd, Fort Worth, TX, USABackground: The purpose of this study was to assess whether differences in central corneal dendritic immune cell densi...

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Autores principales:	Sindt CW, Grout TK, Critser DB, Kern JR, Meadows DL
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2012
Materias:	Ophthalmology RE1-994
Acceso en línea:	https://doaj.org/article/46631dc2b48c40c7ac493f3b2989489f
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spelling	oai:doaj.org-article:46631dc2b48c40c7ac493f3b2989489f2021-12-02T00:35:36ZDendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study1177-54671177-5483https://doaj.org/article/46631dc2b48c40c7ac493f3b2989489f2012-03-01T00:00:00Zhttp://www.dovepress.com/dendritic-immune-cell-densities-in-the-central-cornea-associated-with--a9584https://doaj.org/toc/1177-5467https://doaj.org/toc/1177-5483Christine W Sindt1, Trudy K Grout1, D Brice Critser1, Jami R Kern2, David L Meadows21University of Iowa Hospitals and Clinics, Iowa City, IA; 2Alcon Research Ltd, Fort Worth, TX, USABackground: The purpose of this study was to assess whether differences in central corneal dendritic immune cell densities associated with combinations of soft contact lenses and lens care solutions could be detected by in vivo confocal microscopy.Methods: Participants were adults na&iuml;ve to contact lens wear (n = 10) or who wore soft contact lenses habitually on a daily-wear schedule (n = 38) or on a study-assigned schedule for 30 days with daily disposable silicone hydrogel lenses (n = 15). Central corneas were scanned using an in vivo confocal microscope. Cell densities were compared among groups by demographic parameters, lens materials, and lens care solutions (polyhexamethylene biguanide [PHMB], polyquaternium-1 and myristamidopropyl dimethylamine [PQ/MAPD], peroxide, or blister pack solution [for daily disposable lenses]).Results: Among lens wearers, no associations were observed between immune cell densities and age, gender, or years of lens-wearing experience. Mean cell density was significantly lower (P &lt; 0.01) in nonwearers (29 &plusmn; 23 cells/mm2, n = 10) than in lens wearers (64 &plusmn; 71 cells/mm2, n = 53). Mean cell density was lower (P = 0.21) with traditional polymer lenses (47 &plusmn; 44 cells/mm2, n = 12) than with silicone hydrogel lenses (69 &plusmn; 77 cells/mm2, n = 41). Lowest to highest mean density of immune cells among lens wearers was as follows: PQ/MAPD solution (49 &plusmn; 28 cells/mm2), blister pack solution (63 &plusmn; 81 cells/mm2), PHMB solution (66 &plusmn; 44 cells/mm2), and peroxide solution (85 &plusmn; 112 cells/mm2).Conclusion: In this pilot study, in vivo confocal microscopy was useful for detecting an elevated immune response associated with soft contact lenses, and for identifying lens-related and solution-related immune responses that merit further research.Keywords: Clear Care, in vivo confocal microscopy, Langerhans, Opti-Free Replenish, ReNu Multi-PlusSindt CWGrout TKCritser DBKern JRMeadows DLDove Medical PressarticleOphthalmologyRE1-994ENClinical Ophthalmology, Vol 2012, Iss default, Pp 511-519 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ophthalmology RE1-994
spellingShingle	Ophthalmology RE1-994 Sindt CW Grout TK Critser DB Kern JR Meadows DL Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
description	Christine W Sindt1, Trudy K Grout1, D Brice Critser1, Jami R Kern2, David L Meadows21University of Iowa Hospitals and Clinics, Iowa City, IA; 2Alcon Research Ltd, Fort Worth, TX, USABackground: The purpose of this study was to assess whether differences in central corneal dendritic immune cell densities associated with combinations of soft contact lenses and lens care solutions could be detected by in vivo confocal microscopy.Methods: Participants were adults na&iuml;ve to contact lens wear (n = 10) or who wore soft contact lenses habitually on a daily-wear schedule (n = 38) or on a study-assigned schedule for 30 days with daily disposable silicone hydrogel lenses (n = 15). Central corneas were scanned using an in vivo confocal microscope. Cell densities were compared among groups by demographic parameters, lens materials, and lens care solutions (polyhexamethylene biguanide [PHMB], polyquaternium-1 and myristamidopropyl dimethylamine [PQ/MAPD], peroxide, or blister pack solution [for daily disposable lenses]).Results: Among lens wearers, no associations were observed between immune cell densities and age, gender, or years of lens-wearing experience. Mean cell density was significantly lower (P &lt; 0.01) in nonwearers (29 &plusmn; 23 cells/mm2, n = 10) than in lens wearers (64 &plusmn; 71 cells/mm2, n = 53). Mean cell density was lower (P = 0.21) with traditional polymer lenses (47 &plusmn; 44 cells/mm2, n = 12) than with silicone hydrogel lenses (69 &plusmn; 77 cells/mm2, n = 41). Lowest to highest mean density of immune cells among lens wearers was as follows: PQ/MAPD solution (49 &plusmn; 28 cells/mm2), blister pack solution (63 &plusmn; 81 cells/mm2), PHMB solution (66 &plusmn; 44 cells/mm2), and peroxide solution (85 &plusmn; 112 cells/mm2).Conclusion: In this pilot study, in vivo confocal microscopy was useful for detecting an elevated immune response associated with soft contact lenses, and for identifying lens-related and solution-related immune responses that merit further research.Keywords: Clear Care, in vivo confocal microscopy, Langerhans, Opti-Free Replenish, ReNu Multi-Plus
format	article
author	Sindt CW Grout TK Critser DB Kern JR Meadows DL
author_facet	Sindt CW Grout TK Critser DB Kern JR Meadows DL
author_sort	Sindt CW
title	Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
title_short	Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
title_full	Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
title_fullStr	Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
title_full_unstemmed	Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
title_sort	dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study
publisher	Dove Medical Press
publishDate	2012
url	https://doaj.org/article/46631dc2b48c40c7ac493f3b2989489f
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_version_	1718403612075360256

Dendritic immune cell densities in the central cornea associated with soft contact lens types and lens care solution types: a pilot study

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