Neurite elongation is highly correlated with bulk forward translocation of microtubules

Abstract During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forwa...

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Autores principales:	Ahmad I. M. Athamneh, Yingpei He, Phillip Lamoureux, Lucas Fix, Daniel M. Suter, Kyle E. Miller
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4663e944c62c40b2b7c3b70ccabaadc5
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spelling	oai:doaj.org-article:4663e944c62c40b2b7c3b70ccabaadc52021-12-02T12:30:35ZNeurite elongation is highly correlated with bulk forward translocation of microtubules10.1038/s41598-017-07402-62045-2322https://doaj.org/article/4663e944c62c40b2b7c3b70ccabaadc52017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-07402-6https://doaj.org/toc/2045-2322Abstract During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forward in bulk is unclear. Using fluorescent speckle microscopy (FSM), differential interference contrast (DIC), and phase contrast microscopy, we tracked the movement of MTs, phase dense material, and docked mitochondria in chick sensory and Aplysia bag cell neurons growing rapidly on physiological substrates. In all cases, we find that MTs and other neuritic components move forward in bulk at a rate that on average matches the velocity of neurite elongation. To better understand whether and why MT assembly is required for bulk translocation, we disrupted it with nocodazole. We found this blocked the forward bulk advance of material along the neurite and was paired with a transient increase in axonal tension. This indicates that disruption of MT dynamics interferes with neurite outgrowth, not by disrupting the net assembly of MTs at the growth cone, but rather because it alters the balance of forces that power the bulk forward translocation of MTs.Ahmad I. M. AthamnehYingpei HePhillip LamoureuxLucas FixDaniel M. SuterKyle E. MillerNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ahmad I. M. Athamneh Yingpei He Phillip Lamoureux Lucas Fix Daniel M. Suter Kyle E. Miller Neurite elongation is highly correlated with bulk forward translocation of microtubules
description	Abstract During the development of the nervous system and regeneration following injury, microtubules (MTs) are required for neurite elongation. Whether this elongation occurs primarily through tubulin assembly at the tip of the axon, the transport of individual MTs, or because MTs translocate forward in bulk is unclear. Using fluorescent speckle microscopy (FSM), differential interference contrast (DIC), and phase contrast microscopy, we tracked the movement of MTs, phase dense material, and docked mitochondria in chick sensory and Aplysia bag cell neurons growing rapidly on physiological substrates. In all cases, we find that MTs and other neuritic components move forward in bulk at a rate that on average matches the velocity of neurite elongation. To better understand whether and why MT assembly is required for bulk translocation, we disrupted it with nocodazole. We found this blocked the forward bulk advance of material along the neurite and was paired with a transient increase in axonal tension. This indicates that disruption of MT dynamics interferes with neurite outgrowth, not by disrupting the net assembly of MTs at the growth cone, but rather because it alters the balance of forces that power the bulk forward translocation of MTs.
format	article
author	Ahmad I. M. Athamneh Yingpei He Phillip Lamoureux Lucas Fix Daniel M. Suter Kyle E. Miller
author_facet	Ahmad I. M. Athamneh Yingpei He Phillip Lamoureux Lucas Fix Daniel M. Suter Kyle E. Miller
author_sort	Ahmad I. M. Athamneh
title	Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_short	Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_full	Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_fullStr	Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_full_unstemmed	Neurite elongation is highly correlated with bulk forward translocation of microtubules
title_sort	neurite elongation is highly correlated with bulk forward translocation of microtubules
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/4663e944c62c40b2b7c3b70ccabaadc5
work_keys_str_mv	AT ahmadimathamneh neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules AT yingpeihe neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules AT philliplamoureux neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules AT lucasfix neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules AT danielmsuter neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules AT kyleemiller neuriteelongationishighlycorrelatedwithbulkforwardtranslocationofmicrotubules
_version_	1718394330188611584

Neurite elongation is highly correlated with bulk forward translocation of microtubules

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