A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4

Indium (In) is a critical metal widely used in electronic equipment, and the supply of this precious metal is a major challenge for sustainable development. The use of microorganisms for the recovery of this critical high-tech element has been considered an excellent eco-friendly strategy. The Rhoda...

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Autores principales:	Joana B. Caldeira, Ana Paula Chung, Ana Paula Piedade, Paula V. Morais, Rita Branco
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	DedA family protein indium resistance gene mutation indium bioaccumulation gene complementation Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/4665b309e46e4d449f90b2f0028cf95d
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spelling	oai:doaj.org-article:4665b309e46e4d449f90b2f0028cf95d2021-12-03T14:39:16ZA DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga41664-302X10.3389/fmicb.2021.772127https://doaj.org/article/4665b309e46e4d449f90b2f0028cf95d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.772127/fullhttps://doaj.org/toc/1664-302XIndium (In) is a critical metal widely used in electronic equipment, and the supply of this precious metal is a major challenge for sustainable development. The use of microorganisms for the recovery of this critical high-tech element has been considered an excellent eco-friendly strategy. The Rhodanobacter sp. B2A1Ga4 strain, highly resistant to In, was studied in order to disclose the bacterial mechanisms closely linked to the ability to cope with this metal. The mutation of the gene encoding for a DedA protein homolog, YqaA, affected drastically the In resistance and the cellular metabolic activity of strain Rhodanobacter sp. B2A1Ga4 in presence of this metal. This indicates that this protein plays an important role in its In resistance phenotype. The negative impact of In might be related to the high accumulation of the metal into the mutant cells showing In concentration up to approximately 4-fold higher than the native strain. In addition, the expression of the yqaA gene in this mutant reverted the bacterial phenotype with a significant decrease of In accumulation levels into the cells and an increase of In resistance. Membrane potential measurements showed similar values for native and mutant cells, suggesting that there was no loss of proton-motive force in the mutant cells. The results from this study suggest a potential role of this DedA family protein as a membrane transporter involved in the In efflux process. The mutant strain also has the potential to be used as a biotool in bioaccumulation strategies, for the recovery of In in biomining activities.Joana B. CaldeiraAna Paula ChungAna Paula PiedadePaula V. MoraisRita BrancoFrontiers Media S.A.articleDedA family proteinindium resistancegene mutationindium bioaccumulationgene complementationMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	DedA family protein indium resistance gene mutation indium bioaccumulation gene complementation Microbiology QR1-502
spellingShingle	DedA family protein indium resistance gene mutation indium bioaccumulation gene complementation Microbiology QR1-502 Joana B. Caldeira Ana Paula Chung Ana Paula Piedade Paula V. Morais Rita Branco A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
description	Indium (In) is a critical metal widely used in electronic equipment, and the supply of this precious metal is a major challenge for sustainable development. The use of microorganisms for the recovery of this critical high-tech element has been considered an excellent eco-friendly strategy. The Rhodanobacter sp. B2A1Ga4 strain, highly resistant to In, was studied in order to disclose the bacterial mechanisms closely linked to the ability to cope with this metal. The mutation of the gene encoding for a DedA protein homolog, YqaA, affected drastically the In resistance and the cellular metabolic activity of strain Rhodanobacter sp. B2A1Ga4 in presence of this metal. This indicates that this protein plays an important role in its In resistance phenotype. The negative impact of In might be related to the high accumulation of the metal into the mutant cells showing In concentration up to approximately 4-fold higher than the native strain. In addition, the expression of the yqaA gene in this mutant reverted the bacterial phenotype with a significant decrease of In accumulation levels into the cells and an increase of In resistance. Membrane potential measurements showed similar values for native and mutant cells, suggesting that there was no loss of proton-motive force in the mutant cells. The results from this study suggest a potential role of this DedA family protein as a membrane transporter involved in the In efflux process. The mutant strain also has the potential to be used as a biotool in bioaccumulation strategies, for the recovery of In in biomining activities.
format	article
author	Joana B. Caldeira Ana Paula Chung Ana Paula Piedade Paula V. Morais Rita Branco
author_facet	Joana B. Caldeira Ana Paula Chung Ana Paula Piedade Paula V. Morais Rita Branco
author_sort	Joana B. Caldeira
title	A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
title_short	A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
title_full	A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
title_fullStr	A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
title_full_unstemmed	A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4
title_sort	deda family membrane protein in indium extrusion in rhodanobacter sp. b2a1ga4
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/4665b309e46e4d449f90b2f0028cf95d
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A DedA Family Membrane Protein in Indium Extrusion in Rhodanobacter sp. B2A1Ga4

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