Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.

<h4>Background</h4>Nesfatin-1 is a recently discovered anorexigen encoded in the precursor peptide, nucleobindin-2 (NUCB2) in mammals. To date, nesfatin-1 has not been described in any non-mammalian species, although some information is available in the sequenced genomes of several speci...

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Autores principales:	Ronald Gonzalez, Brent Kerbel, Alexander Chun, Suraj Unniappan
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46670223edf24ab5bdb17586b2451480
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spelling	oai:doaj.org-article:46670223edf24ab5bdb17586b24514802021-11-18T07:02:07ZMolecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.1932-620310.1371/journal.pone.0015201https://doaj.org/article/46670223edf24ab5bdb17586b24514802010-12-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21151928/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Nesfatin-1 is a recently discovered anorexigen encoded in the precursor peptide, nucleobindin-2 (NUCB2) in mammals. To date, nesfatin-1 has not been described in any non-mammalian species, although some information is available in the sequenced genomes of several species. Our objective was to characterize nesfatin-1 in fish.<h4>Methodology/principal findings</h4>In the present study, we employed molecular, immunohistochemical, and physiological studies to characterize the structure, distribution, and appetite regulatory effects of nesfatin-1 in a non-mammalian vertebrate. A very high conservation in NUCB2 sequences, especially in the nesfatin-1 region was found in lower vertebrates. Abundant expression of NUCB2 mRNA was detected in several tissues including the brain and liver of goldfish. Nesfatin-1-like immunoreactive cells are present in the feeding regulatory nucleus of the hypothalamus and in the gastrointestinal tract of goldfish. Approximately 6-fold increase in NUCB2 mRNA levels was found in the liver after 7-day food-deprivation, and a similar increase was also found after short-term fasting. This points toward a possible liver specific role for NUCB2 in the control of metabolism during food-deprivation. Meanwhile, ∼2-fold increase at 1 and 3 h post-feeding and an ∼3-fold reduction after a 7-day food-deprivation was observed in NUCB2 mRNA in the goldfish hypothalamus. In vivo, a single intraperitoneal injection of the full-length native (goldfish; gf) nesfatin-1 at a dose of 50 ng/g body weight induced a 23% reduction of food intake one hour post-injection in goldfish. Furthermore, intracerebroventricular injection of gfnesfatin-1 at a dose of 5 ng/g body weight resulted in ∼50% reduction in food intake.<h4>Conclusions/significance</h4>Our results provide molecular, anatomical and functional evidences to support potential anorectic and metabolic roles for endogenous nesfatin-1 in goldfish. Collectively, we provide novel information on NUCB2 in non-mammals and an anorexigenic role for nesfatin-1 in goldfish.Ronald GonzalezBrent KerbelAlexander ChunSuraj UnniappanPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 12, p e15201 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Ronald Gonzalez Brent Kerbel Alexander Chun Suraj Unniappan Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
description	<h4>Background</h4>Nesfatin-1 is a recently discovered anorexigen encoded in the precursor peptide, nucleobindin-2 (NUCB2) in mammals. To date, nesfatin-1 has not been described in any non-mammalian species, although some information is available in the sequenced genomes of several species. Our objective was to characterize nesfatin-1 in fish.<h4>Methodology/principal findings</h4>In the present study, we employed molecular, immunohistochemical, and physiological studies to characterize the structure, distribution, and appetite regulatory effects of nesfatin-1 in a non-mammalian vertebrate. A very high conservation in NUCB2 sequences, especially in the nesfatin-1 region was found in lower vertebrates. Abundant expression of NUCB2 mRNA was detected in several tissues including the brain and liver of goldfish. Nesfatin-1-like immunoreactive cells are present in the feeding regulatory nucleus of the hypothalamus and in the gastrointestinal tract of goldfish. Approximately 6-fold increase in NUCB2 mRNA levels was found in the liver after 7-day food-deprivation, and a similar increase was also found after short-term fasting. This points toward a possible liver specific role for NUCB2 in the control of metabolism during food-deprivation. Meanwhile, ∼2-fold increase at 1 and 3 h post-feeding and an ∼3-fold reduction after a 7-day food-deprivation was observed in NUCB2 mRNA in the goldfish hypothalamus. In vivo, a single intraperitoneal injection of the full-length native (goldfish; gf) nesfatin-1 at a dose of 50 ng/g body weight induced a 23% reduction of food intake one hour post-injection in goldfish. Furthermore, intracerebroventricular injection of gfnesfatin-1 at a dose of 5 ng/g body weight resulted in ∼50% reduction in food intake.<h4>Conclusions/significance</h4>Our results provide molecular, anatomical and functional evidences to support potential anorectic and metabolic roles for endogenous nesfatin-1 in goldfish. Collectively, we provide novel information on NUCB2 in non-mammals and an anorexigenic role for nesfatin-1 in goldfish.
format	article
author	Ronald Gonzalez Brent Kerbel Alexander Chun Suraj Unniappan
author_facet	Ronald Gonzalez Brent Kerbel Alexander Chun Suraj Unniappan
author_sort	Ronald Gonzalez
title	Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
title_short	Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
title_full	Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
title_fullStr	Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
title_full_unstemmed	Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
title_sort	molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/46670223edf24ab5bdb17586b2451480
work_keys_str_mv	AT ronaldgonzalez molecularcellularandphysiologicalevidencesfortheanorexigenicactionsofnesfatin1ingoldfish AT brentkerbel molecularcellularandphysiologicalevidencesfortheanorexigenicactionsofnesfatin1ingoldfish AT alexanderchun molecularcellularandphysiologicalevidencesfortheanorexigenicactionsofnesfatin1ingoldfish AT surajunniappan molecularcellularandphysiologicalevidencesfortheanorexigenicactionsofnesfatin1ingoldfish
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Molecular, cellular and physiological evidences for the anorexigenic actions of nesfatin-1 in goldfish.

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