Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.

Staphylococcus aureus (S. aureus) is an important etiological organism in chronic and subclinical mastitis in lactating cows. Given the fundamental role the primary bovine mammary epithelial cells (pBMECs) play as a major first line of defense against invading pathogens, their interactions with S. a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiao Wang, Lei Xiu, Qingliang Hu, Xinjie Cui, Bingchun Liu, Lin Tao, Ting Wang, Jingging Wu, Yuan Chen, Yan Chen
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/467354d03ac8458cb7d37a527865b104
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:467354d03ac8458cb7d37a527865b104
record_format	dspace
spelling	oai:doaj.org-article:467354d03ac8458cb7d37a527865b1042021-11-18T08:41:52ZDeep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.1932-620310.1371/journal.pone.0082117https://doaj.org/article/467354d03ac8458cb7d37a527865b1042013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24358144/?tool=EBIhttps://doaj.org/toc/1932-6203Staphylococcus aureus (S. aureus) is an important etiological organism in chronic and subclinical mastitis in lactating cows. Given the fundamental role the primary bovine mammary epithelial cells (pBMECs) play as a major first line of defense against invading pathogens, their interactions with S. aureus was hypothesized to be crucial to the establishment of the latter's infection process. This hypothesis was tested by investigating the global transcriptional responses of pBMECs to three S. aureus strains (S56,S178 and S36) with different virulent factors, using a tag-based high-throughput transcriptome sequencing technique. Approximately 4.9 million total sequence tags were obtained from each of the three S. aureus-infected libraries and the control library. Referenced to the control, 1720, 219, and 427 differentially expressed unique genes were identified in the pBMECs infected with S56, S178 and S36 S. aureus strains respectively. Gene ontology (GO) and pathway analysis of the S56-infected pBMECs referenced to those of the control revealed that the differentially expressed genes in S56-infected pBMECs were significantly involved in inflammatory response, cell signalling pathways and apoptosis. In the same vein, the clustered GO terms of the differentially expressed genes of the S178-infected pBMECs were found to comprise immune responses, metabolism transformation, and apoptosis, while those of the S36-infected pBMECs were primarily involved in cell cycle progression and immune responses. Furthermore, fundamental differences were observed in the levels of expression of immune-related genes in response to treatments with the three S. aureus strains. These differences were especially noted for the expression of important pro-inflammatory molecules, including IL-1α, TNF, EFNB1, IL-8, and EGR1. The transcriptional changes associated with cellular signaling and the inflammatory response in this study may reflect different immunomodulatory mechanisms that underlie the interaction between pBMECs and S. aureus strains during infection by the latter.Xiao WangLei XiuQingliang HuXinjie CuiBingchun LiuLin TaoTing WangJingging WuYuan ChenYan ChenPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82117 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiao Wang Lei Xiu Qingliang Hu Xinjie Cui Bingchun Liu Lin Tao Ting Wang Jingging Wu Yuan Chen Yan Chen Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
description	Staphylococcus aureus (S. aureus) is an important etiological organism in chronic and subclinical mastitis in lactating cows. Given the fundamental role the primary bovine mammary epithelial cells (pBMECs) play as a major first line of defense against invading pathogens, their interactions with S. aureus was hypothesized to be crucial to the establishment of the latter's infection process. This hypothesis was tested by investigating the global transcriptional responses of pBMECs to three S. aureus strains (S56,S178 and S36) with different virulent factors, using a tag-based high-throughput transcriptome sequencing technique. Approximately 4.9 million total sequence tags were obtained from each of the three S. aureus-infected libraries and the control library. Referenced to the control, 1720, 219, and 427 differentially expressed unique genes were identified in the pBMECs infected with S56, S178 and S36 S. aureus strains respectively. Gene ontology (GO) and pathway analysis of the S56-infected pBMECs referenced to those of the control revealed that the differentially expressed genes in S56-infected pBMECs were significantly involved in inflammatory response, cell signalling pathways and apoptosis. In the same vein, the clustered GO terms of the differentially expressed genes of the S178-infected pBMECs were found to comprise immune responses, metabolism transformation, and apoptosis, while those of the S36-infected pBMECs were primarily involved in cell cycle progression and immune responses. Furthermore, fundamental differences were observed in the levels of expression of immune-related genes in response to treatments with the three S. aureus strains. These differences were especially noted for the expression of important pro-inflammatory molecules, including IL-1α, TNF, EFNB1, IL-8, and EGR1. The transcriptional changes associated with cellular signaling and the inflammatory response in this study may reflect different immunomodulatory mechanisms that underlie the interaction between pBMECs and S. aureus strains during infection by the latter.
format	article
author	Xiao Wang Lei Xiu Qingliang Hu Xinjie Cui Bingchun Liu Lin Tao Ting Wang Jingging Wu Yuan Chen Yan Chen
author_facet	Xiao Wang Lei Xiu Qingliang Hu Xinjie Cui Bingchun Liu Lin Tao Ting Wang Jingging Wu Yuan Chen Yan Chen
author_sort	Xiao Wang
title	Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
title_short	Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
title_full	Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
title_fullStr	Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
title_full_unstemmed	Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.
title_sort	deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with staphylococcus aureus stains.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/467354d03ac8458cb7d37a527865b104
work_keys_str_mv	AT xiaowang deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT leixiu deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT qinglianghu deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT xinjiecui deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT bingchunliu deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT lintao deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT tingwang deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT jinggingwu deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT yuanchen deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains AT yanchen deepsequencingbasedtranscriptionalanalysisofbovinemammaryepithelialcellsgeneexpressioninresponsetoinvitroinfectionwithstaphylococcusaureusstains
_version_	1718421442160230400

Deep sequencing-based transcriptional analysis of bovine mammary epithelial cells gene expression in response to in vitro infection with Staphylococcus aureus stains.

Ejemplares similares