RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants

Rose plants are one of the most important horticultural crops, whose commercial value mainly depends on long-distance transportation, and wounding and ethylene are the main factors leading to their quality decline and accelerated senescence in the process. However, underlying molecular mechanisms of...

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Autores principales:	Weikun Jing, Qingcui Zhao, Shuai Zhang, Daxing Zeng, Jiehua Xu, Hougao Zhou, Fenglan Wang, Yang Liu, Yonghong Li
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	rose senescence wound ethylene RhWRKY33 Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/46739ceae3914037bc0e758239aa5e3a
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spelling	oai:doaj.org-article:46739ceae3914037bc0e758239aa5e3a2021-11-05T13:24:09ZRhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants1664-462X10.3389/fpls.2021.726797https://doaj.org/article/46739ceae3914037bc0e758239aa5e3a2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fpls.2021.726797/fullhttps://doaj.org/toc/1664-462XRose plants are one of the most important horticultural crops, whose commercial value mainly depends on long-distance transportation, and wounding and ethylene are the main factors leading to their quality decline and accelerated senescence in the process. However, underlying molecular mechanisms of crosstalk between wounding and ethylene in the regulation of flower senescence remain poorly understood. In relation to this, transcriptome analysis was performed on rose flowers subjected to various treatments, including control, wounding, ethylene, and wounding- and ethylene- (EW) dual treatment. A large number of differentially expressed genes (DEGs) were identified, ranging from 2,442 between the ethylene- and control-treated groups to 4,055 between the EW- and control-treated groups. Using weighted gene co-expression network analysis (WGCNA), we identified a hub gene RhWRKY33 (rchiobhmchr5g0071811), accumulated in the nucleus, where it may function as a transcription factor. Moreover, quantitative reverse transcription PCR (RT-qPCR) results showed that the expression of RhWRKY33 was higher in the wounding-, ethylene, and EW-treated petals than in the control-treated petals. We also functionally characterized the RhWRKY33 gene through virus-induced gene silencing (VIGS). The silencing of RhWRKY33 significantly delayed the senescence process in the different treatments (control, wounding, ethylene, and EW). Meanwhile, we found that the effect of RhWRKY33-silenced petals under ethylene and EW dual-treatment were stronger than those under wounding treatment in delaying the petal senescence process, implying that RhWRKY33 is closely involved with ethylene and wounding mediated petal senescence. Overall, the results indicate that RhWRKY33 positively regulates the onset of floral senescence mediated by both ethylene and wounding signaling, but relies heavily on ethylene signaling.Weikun JingWeikun JingQingcui ZhaoQingcui ZhaoShuai ZhangShuai ZhangDaxing ZengJiehua XuHougao ZhouFenglan WangYang LiuYonghong LiFrontiers Media S.A.articlerosesenescencewoundethyleneRhWRKY33Plant cultureSB1-1110ENFrontiers in Plant Science, Vol 12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	rose senescence wound ethylene RhWRKY33 Plant culture SB1-1110
spellingShingle	rose senescence wound ethylene RhWRKY33 Plant culture SB1-1110 Weikun Jing Weikun Jing Qingcui Zhao Qingcui Zhao Shuai Zhang Shuai Zhang Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Yang Liu Yonghong Li RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
description	Rose plants are one of the most important horticultural crops, whose commercial value mainly depends on long-distance transportation, and wounding and ethylene are the main factors leading to their quality decline and accelerated senescence in the process. However, underlying molecular mechanisms of crosstalk between wounding and ethylene in the regulation of flower senescence remain poorly understood. In relation to this, transcriptome analysis was performed on rose flowers subjected to various treatments, including control, wounding, ethylene, and wounding- and ethylene- (EW) dual treatment. A large number of differentially expressed genes (DEGs) were identified, ranging from 2,442 between the ethylene- and control-treated groups to 4,055 between the EW- and control-treated groups. Using weighted gene co-expression network analysis (WGCNA), we identified a hub gene RhWRKY33 (rchiobhmchr5g0071811), accumulated in the nucleus, where it may function as a transcription factor. Moreover, quantitative reverse transcription PCR (RT-qPCR) results showed that the expression of RhWRKY33 was higher in the wounding-, ethylene, and EW-treated petals than in the control-treated petals. We also functionally characterized the RhWRKY33 gene through virus-induced gene silencing (VIGS). The silencing of RhWRKY33 significantly delayed the senescence process in the different treatments (control, wounding, ethylene, and EW). Meanwhile, we found that the effect of RhWRKY33-silenced petals under ethylene and EW dual-treatment were stronger than those under wounding treatment in delaying the petal senescence process, implying that RhWRKY33 is closely involved with ethylene and wounding mediated petal senescence. Overall, the results indicate that RhWRKY33 positively regulates the onset of floral senescence mediated by both ethylene and wounding signaling, but relies heavily on ethylene signaling.
format	article
author	Weikun Jing Weikun Jing Qingcui Zhao Qingcui Zhao Shuai Zhang Shuai Zhang Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Yang Liu Yonghong Li
author_facet	Weikun Jing Weikun Jing Qingcui Zhao Qingcui Zhao Shuai Zhang Shuai Zhang Daxing Zeng Jiehua Xu Hougao Zhou Fenglan Wang Yang Liu Yonghong Li
author_sort	Weikun Jing
title	RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
title_short	RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
title_full	RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
title_fullStr	RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
title_full_unstemmed	RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants
title_sort	rhwrky33 positively regulates onset of floral senescence by responding to wounding- and ethylene-signaling in rose plants
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/46739ceae3914037bc0e758239aa5e3a
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RhWRKY33 Positively Regulates Onset of Floral Senescence by Responding to Wounding- and Ethylene-Signaling in Rose Plants

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