Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice

Abstract Brown adipose tissue (BAT) is the primary non-shivering thermogenesis organ in mammals, which plays essential roles in maintaining the body temperature of infants. Although the development of BAT during embryogenesis has been well addressed in rodents, how BAT grows after birth remains unkn...

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Autores principales:	Steven G. Negron, A. Gulhan Ercan-Sencicek, Jessica Freed, Madeline Walters, Zhiqiang Lin
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/467619470df544eb8f9d2a6ca2bf71f3
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spelling	oai:doaj.org-article:467619470df544eb8f9d2a6ca2bf71f32021-12-02T11:41:40ZBoth proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice10.1038/s41598-020-77362-x2045-2322https://doaj.org/article/467619470df544eb8f9d2a6ca2bf71f32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77362-xhttps://doaj.org/toc/2045-2322Abstract Brown adipose tissue (BAT) is the primary non-shivering thermogenesis organ in mammals, which plays essential roles in maintaining the body temperature of infants. Although the development of BAT during embryogenesis has been well addressed in rodents, how BAT grows after birth remains unknown. Using mouse interscapular BAT (iBAT) as an example, we studied the cellular and molecular mechanisms that regulate postnatal BAT growth. By analyzing the developmental dynamics of brown adipocytes (BAs), we found that BAs size enlargement partially accounts for iBAT growth. By investigating the BAs cell cycle activities, we confirmed the presence of proliferative BAs in the neonatal mice. Two weeks after birth, most of the BAs exit cell cycle, and the further expansion of the BAT was mainly due to lipogenesis-mediated BAs volume increase. Microscopy and fluorescence-activated cell sorting analyses suggest that most BAs are mononuclear and diploid. Based on the developmental dynamics of brown adipocytes, we propose that the murine iBAT has two different growth phases between birth and weaning: increase of BAs size and number in the first two weeks, and BAs size enlargement thereafter. In summary, our data demonstrate that both lipogenesis and proliferation of BAs contribute to postnatal iBAT growth in mice.Steven G. NegronA. Gulhan Ercan-SencicekJessica FreedMadeline WaltersZhiqiang LinNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-11 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Steven G. Negron A. Gulhan Ercan-Sencicek Jessica Freed Madeline Walters Zhiqiang Lin Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
description	Abstract Brown adipose tissue (BAT) is the primary non-shivering thermogenesis organ in mammals, which plays essential roles in maintaining the body temperature of infants. Although the development of BAT during embryogenesis has been well addressed in rodents, how BAT grows after birth remains unknown. Using mouse interscapular BAT (iBAT) as an example, we studied the cellular and molecular mechanisms that regulate postnatal BAT growth. By analyzing the developmental dynamics of brown adipocytes (BAs), we found that BAs size enlargement partially accounts for iBAT growth. By investigating the BAs cell cycle activities, we confirmed the presence of proliferative BAs in the neonatal mice. Two weeks after birth, most of the BAs exit cell cycle, and the further expansion of the BAT was mainly due to lipogenesis-mediated BAs volume increase. Microscopy and fluorescence-activated cell sorting analyses suggest that most BAs are mononuclear and diploid. Based on the developmental dynamics of brown adipocytes, we propose that the murine iBAT has two different growth phases between birth and weaning: increase of BAs size and number in the first two weeks, and BAs size enlargement thereafter. In summary, our data demonstrate that both lipogenesis and proliferation of BAs contribute to postnatal iBAT growth in mice.
format	article
author	Steven G. Negron A. Gulhan Ercan-Sencicek Jessica Freed Madeline Walters Zhiqiang Lin
author_facet	Steven G. Negron A. Gulhan Ercan-Sencicek Jessica Freed Madeline Walters Zhiqiang Lin
author_sort	Steven G. Negron
title	Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
title_short	Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
title_full	Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
title_fullStr	Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
title_full_unstemmed	Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
title_sort	both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/467619470df544eb8f9d2a6ca2bf71f3
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Both proliferation and lipogenesis of brown adipocytes contribute to postnatal brown adipose tissue growth in mice

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