Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L.
Abstract Background Melatonin 2-hydroxylase (M2H) is the first enzyme in the catabolism pathway of melatonin, which catalyzes the production of 2-hydroxymelatonin (2-OHM) from melatonin. The content of 2-hydroxymelatonin in plants is much higher than that of melatonin. So M2H may be a key enzyme in...
Guardado en:
| Autores principales: | , , , , , , , , , , , , , , , |
|---|---|
| Formato: | article |
| Lenguaje: | EN |
| Publicado: |
BMC
2021
|
| Materias: | |
| Acceso en línea: | https://doaj.org/article/467919567c5f459498d250546cb765de |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| id |
oai:doaj.org-article:467919567c5f459498d250546cb765de |
|---|---|
| record_format |
dspace |
| spelling |
oai:doaj.org-article:467919567c5f459498d250546cb765de2021-11-08T11:04:23ZGenome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L.10.1186/s40659-021-00358-y0717-6287https://doaj.org/article/467919567c5f459498d250546cb765de2021-11-01T00:00:00Zhttps://doi.org/10.1186/s40659-021-00358-yhttps://doaj.org/toc/0717-6287Abstract Background Melatonin 2-hydroxylase (M2H) is the first enzyme in the catabolism pathway of melatonin, which catalyzes the production of 2-hydroxymelatonin (2-OHM) from melatonin. The content of 2-hydroxymelatonin in plants is much higher than that of melatonin. So M2H may be a key enzyme in the metabolic pathway of melatonin. Method We conducted a systematic analysis of the M2H gene family in Gossypium hirsutum based on the whole genome sequence by integrating the structural characteristics, phylogenetic relationships, expression profile, and biological stress of the members of the Gossypium hirsutum M2H gene family. Result We identified 265 M2H genes in the whole genome of Gossypium hirsutum, which were divided into 7 clades (clades I-VII) according to phylogenetic analysis. Most M2H members in each group had similar motif composition and gene structure characteristics. More than half of GhM2H members contain ABA-responsive elements and MeJA-responsive elements. Under different stress conditions, the expression levels of the gene changed, indicating that GhM2H members were involved in the regulation of abiotic stress. Some genes in the GhM2H family were involved in regulating melatonin levels in cotton under salt stress, and some genes were regulated by exogenous melatonin. Conclusion This study is helpful to explore the function of GhM2H, the downstream metabolism gene of melatonin in cotton, and lay the foundation for better exploring the molecular mechanism of melatonin improving cotton's response to abiotic stress.Yuexin ZhangJing WangXiugui ChenXuke LuDelong WangJunjuan WangShuai WangChao ChenLixue GuoWaqar Afzal MalikYapeng FanCun RuiRuifeng CuiQinqin WangYuqian LeiWuwei YeBMCarticleM2H2-hydroxymelatoninMelatoninGene familyAbiotic stressCottonBiology (General)QH301-705.5ENBiological Research, Vol 54, Iss 1, Pp 1-17 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
M2H 2-hydroxymelatonin Melatonin Gene family Abiotic stress Cotton Biology (General) QH301-705.5 |
| spellingShingle |
M2H 2-hydroxymelatonin Melatonin Gene family Abiotic stress Cotton Biology (General) QH301-705.5 Yuexin Zhang Jing Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Waqar Afzal Malik Yapeng Fan Cun Rui Ruifeng Cui Qinqin Wang Yuqian Lei Wuwei Ye Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| description |
Abstract Background Melatonin 2-hydroxylase (M2H) is the first enzyme in the catabolism pathway of melatonin, which catalyzes the production of 2-hydroxymelatonin (2-OHM) from melatonin. The content of 2-hydroxymelatonin in plants is much higher than that of melatonin. So M2H may be a key enzyme in the metabolic pathway of melatonin. Method We conducted a systematic analysis of the M2H gene family in Gossypium hirsutum based on the whole genome sequence by integrating the structural characteristics, phylogenetic relationships, expression profile, and biological stress of the members of the Gossypium hirsutum M2H gene family. Result We identified 265 M2H genes in the whole genome of Gossypium hirsutum, which were divided into 7 clades (clades I-VII) according to phylogenetic analysis. Most M2H members in each group had similar motif composition and gene structure characteristics. More than half of GhM2H members contain ABA-responsive elements and MeJA-responsive elements. Under different stress conditions, the expression levels of the gene changed, indicating that GhM2H members were involved in the regulation of abiotic stress. Some genes in the GhM2H family were involved in regulating melatonin levels in cotton under salt stress, and some genes were regulated by exogenous melatonin. Conclusion This study is helpful to explore the function of GhM2H, the downstream metabolism gene of melatonin in cotton, and lay the foundation for better exploring the molecular mechanism of melatonin improving cotton's response to abiotic stress. |
| format |
article |
| author |
Yuexin Zhang Jing Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Waqar Afzal Malik Yapeng Fan Cun Rui Ruifeng Cui Qinqin Wang Yuqian Lei Wuwei Ye |
| author_facet |
Yuexin Zhang Jing Wang Xiugui Chen Xuke Lu Delong Wang Junjuan Wang Shuai Wang Chao Chen Lixue Guo Waqar Afzal Malik Yapeng Fan Cun Rui Ruifeng Cui Qinqin Wang Yuqian Lei Wuwei Ye |
| author_sort |
Yuexin Zhang |
| title |
Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| title_short |
Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| title_full |
Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| title_fullStr |
Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| title_full_unstemmed |
Genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene GhM2H in Gossypium hirsutum L. |
| title_sort |
genome-wide identification and characteristic analysis of the downstream melatonin metabolism gene ghm2h in gossypium hirsutum l. |
| publisher |
BMC |
| publishDate |
2021 |
| url |
https://doaj.org/article/467919567c5f459498d250546cb765de |
| work_keys_str_mv |
AT yuexinzhang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT jingwang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT xiuguichen genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT xukelu genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT delongwang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT junjuanwang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT shuaiwang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT chaochen genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT lixueguo genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT waqarafzalmalik genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT yapengfan genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT cunrui genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT ruifengcui genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT qinqinwang genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT yuqianlei genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml AT wuweiye genomewideidentificationandcharacteristicanalysisofthedownstreammelatoninmetabolismgeneghm2hingossypiumhirsutuml |
| _version_ |
1718442418554011648 |