Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study

Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic <i>Escherichia coli</i> (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The...

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Autores principales:	Manuel G. Ballesteros-Monrreal, Margarita M. P. Arenas-Hernández, Edwin Barrios-Villa, Josue Juarez, Maritza Lizeth Álvarez-Ainza, Pablo Taboada, Rafael De la Rosa-López, Enrique Bolado-Martínez, Dora Valencia
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	urinary tract infection UPEC morphotypes UPEC virulence Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/467ec83045504e1bb8bbb5f7dcc50e7f
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spelling	oai:doaj.org-article:467ec83045504e1bb8bbb5f7dcc50e7f2021-11-25T18:25:33ZBacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study10.3390/microorganisms91123812076-2607https://doaj.org/article/467ec83045504e1bb8bbb5f7dcc50e7f2021-11-01T00:00:00Zhttps://www.mdpi.com/2076-2607/9/11/2381https://doaj.org/toc/2076-2607Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic <i>Escherichia coli</i> (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where <i>E. coli</i> was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were <i>fimH</i> (100%), <i>fliCD</i> (90%), and <i>sfaD/focC</i> (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.Manuel G. Ballesteros-MonrrealMargarita M. P. Arenas-HernándezEdwin Barrios-VillaJosue JuarezMaritza Lizeth Álvarez-AinzaPablo TaboadaRafael De la Rosa-LópezEnrique Bolado-MartínezDora ValenciaMDPI AGarticleurinary tract infectionUPEC morphotypesUPEC virulenceBiology (General)QH301-705.5ENMicroorganisms, Vol 9, Iss 2381, p 2381 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	urinary tract infection UPEC morphotypes UPEC virulence Biology (General) QH301-705.5
spellingShingle	urinary tract infection UPEC morphotypes UPEC virulence Biology (General) QH301-705.5 Manuel G. Ballesteros-Monrreal Margarita M. P. Arenas-Hernández Edwin Barrios-Villa Josue Juarez Maritza Lizeth Álvarez-Ainza Pablo Taboada Rafael De la Rosa-López Enrique Bolado-Martínez Dora Valencia Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
description	Urinary tract infections (UTIs) belong to the most common pathologies in Mexico and are mainly caused by Uropathogenic <i>Escherichia coli</i> (UPEC). UPEC possesses a wide diversity of virulence factors that allow it to carry out its pathogenesis mechanism in the urinary tract (UT). The development of morphotypes in UT represents an important feature of UPEC because it is associated with complications in diagnosis of UTI. The aim of this study was to determine the presence of bacterial morphotypes, virulence genes, virulence phenotypes, antibiotic resistant, and phylogenetic groups in clinical isolates of UPEC obtained from women in Sonora, Mexico. Forty UPEC isolates were obtained, and urine morphotypes were observed in 65% of the urine samples from where <i>E. coli</i> was isolated. Phylogenetic group B2 was the most prevalent. The most frequent virulence genes were <i>fimH</i> (100%), <i>fliCD</i> (90%), and <i>sfaD/focC</i> (72%). Biofilm formation (100%) and motility (98%) were the most prevalent phenotypes. Clinical isolates showed high resistance to aminoglycosides and β-lactams antibiotics. These data suggest that the search for morphotypes in urine sediment must be incorporated in the urinalysis procedure and also that clinical isolates of UPEC in this study can cause upper, lower, and recurrent UTI.
format	article
author	Manuel G. Ballesteros-Monrreal Margarita M. P. Arenas-Hernández Edwin Barrios-Villa Josue Juarez Maritza Lizeth Álvarez-Ainza Pablo Taboada Rafael De la Rosa-López Enrique Bolado-Martínez Dora Valencia
author_facet	Manuel G. Ballesteros-Monrreal Margarita M. P. Arenas-Hernández Edwin Barrios-Villa Josue Juarez Maritza Lizeth Álvarez-Ainza Pablo Taboada Rafael De la Rosa-López Enrique Bolado-Martínez Dora Valencia
author_sort	Manuel G. Ballesteros-Monrreal
title	Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
title_short	Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
title_full	Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
title_fullStr	Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
title_full_unstemmed	Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study
title_sort	bacterial morphotypes as important trait for uropathogenic <i>e. coli</i> diagnostic; a virulence-phenotype-phylogeny study
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/467ec83045504e1bb8bbb5f7dcc50e7f
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Bacterial Morphotypes as Important Trait for Uropathogenic <i>E. coli</i> Diagnostic; a Virulence-Phenotype-Phylogeny Study

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