Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism

In this work, the effect of thyroxine on energy and oxidative metabolism in the mitochondria of the rat heart was studied. Hyperthyroidism was observed in experimental animals after chronic administration of T<sub>4</sub>, which was accompanied by an increase in serum concentrations of f...

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Autores principales:	Natalya Venediktova, Ilya Solomadin, Anna Nikiforova, Vlada Starinets, Galina Mironova
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mitochondria energy metabolism respiratory complexes thyroid hormones oxidative stress mitochondrial dysfunction Biology (General) QH301-705.5 Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/467f2588c337426eaa2b6ab58d7ddaf6
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id	oai:doaj.org-article:467f2588c337426eaa2b6ab58d7ddaf6
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spelling	oai:doaj.org-article:467f2588c337426eaa2b6ab58d7ddaf62021-11-11T17:12:12ZFunctional State of Rat Heart Mitochondria in Experimental Hyperthyroidism10.3390/ijms2221117441422-00671661-6596https://doaj.org/article/467f2588c337426eaa2b6ab58d7ddaf62021-10-01T00:00:00Zhttps://www.mdpi.com/1422-0067/22/21/11744https://doaj.org/toc/1661-6596https://doaj.org/toc/1422-0067In this work, the effect of thyroxine on energy and oxidative metabolism in the mitochondria of the rat heart was studied. Hyperthyroidism was observed in experimental animals after chronic administration of T<sub>4</sub>, which was accompanied by an increase in serum concentrations of free triiodothyronine (T<sub>3</sub>) and thyroxine (T<sub>4</sub>) by 1.8 and 3.4 times, respectively. The hyperthyroid rats (HR) had hypertrophy of the heart. In HR, there was a change in the oxygen consumption in the mitochondria of the heart, especially when using palmitoylcarnitine. The assay of respiratory chain enzymes revealed that the activities of complexes I, I + III, III, IV increased, whereas the activities of complexes II, II + III decreased in heart mitochondria of the experimental animals. It was shown that the level of respiratory complexes of the electron transport chain in hyperthyroid rats increased, except for complex V, the quantity of which was reduced. The development of oxidative stress in HR was observed: an increase in the hydrogen peroxide production rate, increase in lipid peroxidation and reduced glutathione. The activity of superoxide dismutase in the heart of HR was higher than in the control. At the same time, the activity of glutathione peroxidase decreased. The obtained data indicate that increased concentrations of thyroid hormones lead to changes in energy metabolism and the development of oxidative stress in the heart of rats, which in turn contributes to heart dysfunction.Natalya VenediktovaIlya SolomadinAnna NikiforovaVlada StarinetsGalina MironovaMDPI AGarticlemitochondriaenergy metabolismrespiratory complexesthyroid hormonesoxidative stressmitochondrial dysfunctionBiology (General)QH301-705.5ChemistryQD1-999ENInternational Journal of Molecular Sciences, Vol 22, Iss 11744, p 11744 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mitochondria energy metabolism respiratory complexes thyroid hormones oxidative stress mitochondrial dysfunction Biology (General) QH301-705.5 Chemistry QD1-999
spellingShingle	mitochondria energy metabolism respiratory complexes thyroid hormones oxidative stress mitochondrial dysfunction Biology (General) QH301-705.5 Chemistry QD1-999 Natalya Venediktova Ilya Solomadin Anna Nikiforova Vlada Starinets Galina Mironova Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
description	In this work, the effect of thyroxine on energy and oxidative metabolism in the mitochondria of the rat heart was studied. Hyperthyroidism was observed in experimental animals after chronic administration of T<sub>4</sub>, which was accompanied by an increase in serum concentrations of free triiodothyronine (T<sub>3</sub>) and thyroxine (T<sub>4</sub>) by 1.8 and 3.4 times, respectively. The hyperthyroid rats (HR) had hypertrophy of the heart. In HR, there was a change in the oxygen consumption in the mitochondria of the heart, especially when using palmitoylcarnitine. The assay of respiratory chain enzymes revealed that the activities of complexes I, I + III, III, IV increased, whereas the activities of complexes II, II + III decreased in heart mitochondria of the experimental animals. It was shown that the level of respiratory complexes of the electron transport chain in hyperthyroid rats increased, except for complex V, the quantity of which was reduced. The development of oxidative stress in HR was observed: an increase in the hydrogen peroxide production rate, increase in lipid peroxidation and reduced glutathione. The activity of superoxide dismutase in the heart of HR was higher than in the control. At the same time, the activity of glutathione peroxidase decreased. The obtained data indicate that increased concentrations of thyroid hormones lead to changes in energy metabolism and the development of oxidative stress in the heart of rats, which in turn contributes to heart dysfunction.
format	article
author	Natalya Venediktova Ilya Solomadin Anna Nikiforova Vlada Starinets Galina Mironova
author_facet	Natalya Venediktova Ilya Solomadin Anna Nikiforova Vlada Starinets Galina Mironova
author_sort	Natalya Venediktova
title	Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
title_short	Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
title_full	Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
title_fullStr	Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
title_full_unstemmed	Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism
title_sort	functional state of rat heart mitochondria in experimental hyperthyroidism
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/467f2588c337426eaa2b6ab58d7ddaf6
work_keys_str_mv	AT natalyavenediktova functionalstateofratheartmitochondriainexperimentalhyperthyroidism AT ilyasolomadin functionalstateofratheartmitochondriainexperimentalhyperthyroidism AT annanikiforova functionalstateofratheartmitochondriainexperimentalhyperthyroidism AT vladastarinets functionalstateofratheartmitochondriainexperimentalhyperthyroidism AT galinamironova functionalstateofratheartmitochondriainexperimentalhyperthyroidism
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Functional State of Rat Heart Mitochondria in Experimental Hyperthyroidism

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