Lasso Proteins—Unifying Cysteine Knots and Miniproteins

Complex lasso proteins are a recently identified class of biological compounds that are present in considerable fraction of proteins with disulfide bridges. In this work, we look at complex lasso proteins as a generalization of well-known cysteine knots and miniproteins (lasso peptides). In particul...

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Autores principales:	Bartosz Ambroży Greń, Pawel Dabrowski-Tumanski, Wanda Niemyska, Joanna Ida Sulkowska
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	topology lasso proteins Organic chemistry QD241-441
Acceso en línea:	https://doaj.org/article/467f55480cd4499fad788c704f888529
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spelling	oai:doaj.org-article:467f55480cd4499fad788c704f8885292021-11-25T18:49:06ZLasso Proteins—Unifying Cysteine Knots and Miniproteins10.3390/polym132239882073-4360https://doaj.org/article/467f55480cd4499fad788c704f8885292021-11-01T00:00:00Zhttps://www.mdpi.com/2073-4360/13/22/3988https://doaj.org/toc/2073-4360Complex lasso proteins are a recently identified class of biological compounds that are present in considerable fraction of proteins with disulfide bridges. In this work, we look at complex lasso proteins as a generalization of well-known cysteine knots and miniproteins (lasso peptides). In particular, we show that complex lasso proteins with the same crucial topological features—cysteine knots and lasso peptides—are antimicrobial proteins, which suggests that they act as a molecular plug. Based on an analysis of the stability of the lasso piercing residue, we also introduce a method to determine which lasso motif is potentially functional. Using this method, we show that the lasso motif in antimicrobial proteins, as well in that in cytokines, is functionally relevant. We also study the evolution of lasso motifs, their conservation, and the usefulness of the lasso fingerprint, which extracts all topologically non-triviality concerning covalent loops. The work is completed by the presentation of extensive statistics on complex lasso proteins to analyze, in particular, the strange propensity for “negative” piercings. We also identify 21 previously unknown complex lasso proteins with an ester and a thioester bridge.Bartosz Ambroży GreńPawel Dabrowski-TumanskiWanda NiemyskaJoanna Ida SulkowskaMDPI AGarticletopologylassoproteinsOrganic chemistryQD241-441ENPolymers, Vol 13, Iss 3988, p 3988 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	topology lasso proteins Organic chemistry QD241-441
spellingShingle	topology lasso proteins Organic chemistry QD241-441 Bartosz Ambroży Greń Pawel Dabrowski-Tumanski Wanda Niemyska Joanna Ida Sulkowska Lasso Proteins—Unifying Cysteine Knots and Miniproteins
description	Complex lasso proteins are a recently identified class of biological compounds that are present in considerable fraction of proteins with disulfide bridges. In this work, we look at complex lasso proteins as a generalization of well-known cysteine knots and miniproteins (lasso peptides). In particular, we show that complex lasso proteins with the same crucial topological features—cysteine knots and lasso peptides—are antimicrobial proteins, which suggests that they act as a molecular plug. Based on an analysis of the stability of the lasso piercing residue, we also introduce a method to determine which lasso motif is potentially functional. Using this method, we show that the lasso motif in antimicrobial proteins, as well in that in cytokines, is functionally relevant. We also study the evolution of lasso motifs, their conservation, and the usefulness of the lasso fingerprint, which extracts all topologically non-triviality concerning covalent loops. The work is completed by the presentation of extensive statistics on complex lasso proteins to analyze, in particular, the strange propensity for “negative” piercings. We also identify 21 previously unknown complex lasso proteins with an ester and a thioester bridge.
format	article
author	Bartosz Ambroży Greń Pawel Dabrowski-Tumanski Wanda Niemyska Joanna Ida Sulkowska
author_facet	Bartosz Ambroży Greń Pawel Dabrowski-Tumanski Wanda Niemyska Joanna Ida Sulkowska
author_sort	Bartosz Ambroży Greń
title	Lasso Proteins—Unifying Cysteine Knots and Miniproteins
title_short	Lasso Proteins—Unifying Cysteine Knots and Miniproteins
title_full	Lasso Proteins—Unifying Cysteine Knots and Miniproteins
title_fullStr	Lasso Proteins—Unifying Cysteine Knots and Miniproteins
title_full_unstemmed	Lasso Proteins—Unifying Cysteine Knots and Miniproteins
title_sort	lasso proteins—unifying cysteine knots and miniproteins
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/467f55480cd4499fad788c704f888529
work_keys_str_mv	AT bartoszambrozygren lassoproteinsunifyingcysteineknotsandminiproteins AT paweldabrowskitumanski lassoproteinsunifyingcysteineknotsandminiproteins AT wandaniemyska lassoproteinsunifyingcysteineknotsandminiproteins AT joannaidasulkowska lassoproteinsunifyingcysteineknotsandminiproteins
_version_	1718410644199309312

Lasso Proteins—Unifying Cysteine Knots and Miniproteins

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