Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport

Abstract Manganese has long been employed as a T1-shortening agent in magnetic resonance imaging (MRI) applications, but these techniques are limited by the biotoxicity of bulk-manganese. Positron emission tomography (PET) offers superior contrast sensitivity compared with MRI, and recent preclinica...

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Autores principales:	Stephen A. Graves, Reinier Hernandez, Hector F. Valdovinos, Paul A. Ellison, Jonathan W. Engle, Todd E. Barnhart, Weibo Cai, Robert J. Nickles
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46829e4f4a5a44178a2d69db0547dc99
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spelling	oai:doaj.org-article:46829e4f4a5a44178a2d69db0547dc992021-12-02T16:06:45ZPreparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport10.1038/s41598-017-03202-02045-2322https://doaj.org/article/46829e4f4a5a44178a2d69db0547dc992017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03202-0https://doaj.org/toc/2045-2322Abstract Manganese has long been employed as a T1-shortening agent in magnetic resonance imaging (MRI) applications, but these techniques are limited by the biotoxicity of bulk-manganese. Positron emission tomography (PET) offers superior contrast sensitivity compared with MRI, and recent preclinical PET studies employing 52gMn (t1/2: 5.6 d, β+: 29%) show promise for a variety of applications including cell tracking, neural tract tracing, immunoPET, and functional β-cell mass quantification. The half-life and confounding gamma emissions of 52gMn are prohibitive to clinical translation, but the short-lived 51Mn (t1/2: 46 min, β+: 97%) represents a viable alternative. This work develops methods to produce 51Mn on low-energy medical cyclotrons, characterizes the in vivo behavior of 51MnCl2 in mice, and performs preliminary human dosimetry predictions. 51Mn was produced by proton irradiation of electrodeposited isotopically-enriched 54Fe targets. Radiochemically isolated 51MnCl2 was intravenously administered to ICR mice which were scanned by dynamic and static PET, followed by ex vivo gamma counting. Rapid blood clearance was observed with stable uptake in the pancreas, kidneys, liver, heart, and salivary gland. Dosimetry calculations predict that 370 MBq of 51Mn in an adult human male would yield an effective dose equivalent of approximately 13.5 mSv, roughly equivalent to a clinical [18F]-FDG procedure.Stephen A. GravesReinier HernandezHector F. ValdovinosPaul A. EllisonJonathan W. EngleTodd E. BarnhartWeibo CaiRobert J. NicklesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Stephen A. Graves Reinier Hernandez Hector F. Valdovinos Paul A. Ellison Jonathan W. Engle Todd E. Barnhart Weibo Cai Robert J. Nickles Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
description	Abstract Manganese has long been employed as a T1-shortening agent in magnetic resonance imaging (MRI) applications, but these techniques are limited by the biotoxicity of bulk-manganese. Positron emission tomography (PET) offers superior contrast sensitivity compared with MRI, and recent preclinical PET studies employing 52gMn (t1/2: 5.6 d, β+: 29%) show promise for a variety of applications including cell tracking, neural tract tracing, immunoPET, and functional β-cell mass quantification. The half-life and confounding gamma emissions of 52gMn are prohibitive to clinical translation, but the short-lived 51Mn (t1/2: 46 min, β+: 97%) represents a viable alternative. This work develops methods to produce 51Mn on low-energy medical cyclotrons, characterizes the in vivo behavior of 51MnCl2 in mice, and performs preliminary human dosimetry predictions. 51Mn was produced by proton irradiation of electrodeposited isotopically-enriched 54Fe targets. Radiochemically isolated 51MnCl2 was intravenously administered to ICR mice which were scanned by dynamic and static PET, followed by ex vivo gamma counting. Rapid blood clearance was observed with stable uptake in the pancreas, kidneys, liver, heart, and salivary gland. Dosimetry calculations predict that 370 MBq of 51Mn in an adult human male would yield an effective dose equivalent of approximately 13.5 mSv, roughly equivalent to a clinical [18F]-FDG procedure.
format	article
author	Stephen A. Graves Reinier Hernandez Hector F. Valdovinos Paul A. Ellison Jonathan W. Engle Todd E. Barnhart Weibo Cai Robert J. Nickles
author_facet	Stephen A. Graves Reinier Hernandez Hector F. Valdovinos Paul A. Ellison Jonathan W. Engle Todd E. Barnhart Weibo Cai Robert J. Nickles
author_sort	Stephen A. Graves
title	Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
title_short	Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
title_full	Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
title_fullStr	Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
title_full_unstemmed	Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport
title_sort	preparation and in vivo characterization of 51mncl2 as pet tracer of ca2+ channel-mediated transport
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/46829e4f4a5a44178a2d69db0547dc99
work_keys_str_mv	AT stephenagraves preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT reinierhernandez preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT hectorfvaldovinos preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT paulaellison preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT jonathanwengle preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT toddebarnhart preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT weibocai preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport AT robertjnickles preparationandinvivocharacterizationof51mncl2aspettracerofca2channelmediatedtransport
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Preparation and in vivo characterization of 51MnCl2 as PET tracer of Ca2+ channel-mediated transport

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