Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells

Abstract Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been inve...

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Autores principales:	Po-Yen Lee, Yu-Hung Lai, Po-Len Liu, Ching-Chih Liu, Chia-Cheng Su, Fang-Yen Chiu, Wei-Chung Cheng, Shiuh-Liang Hsu, Kai-Chun Cheng, Li-Yi Chiu, Tzu-En Kao, Chia-Ching Lin, Yo-Chen Chang, Shu-Chi Wang, Chia-Yang Li
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/4685db666c914a6b81d9725b14670eba
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spelling	oai:doaj.org-article:4685db666c914a6b81d9725b14670eba2021-12-02T15:15:40ZToxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells10.1038/s41598-021-98005-92045-2322https://doaj.org/article/4685db666c914a6b81d9725b14670eba2021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98005-9https://doaj.org/toc/2045-2322Abstract Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema.Po-Yen LeeYu-Hung LaiPo-Len LiuChing-Chih LiuChia-Cheng SuFang-Yen ChiuWei-Chung ChengShiuh-Liang HsuKai-Chun ChengLi-Yi ChiuTzu-En KaoChia-Ching LinYo-Chen ChangShu-Chi WangChia-Yang LiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Po-Yen Lee Yu-Hung Lai Po-Len Liu Ching-Chih Liu Chia-Cheng Su Fang-Yen Chiu Wei-Chung Cheng Shiuh-Liang Hsu Kai-Chun Cheng Li-Yi Chiu Tzu-En Kao Chia-Ching Lin Yo-Chen Chang Shu-Chi Wang Chia-Yang Li Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
description	Abstract Amantadine hydrochloride (HCl) is commonly prescribed for treating influenza A virus infection and Parkinson’s disease. Recently, several studies have indicated that the use of amantadine HCl is associated with corneal edema; however, the cytotoxic effect of amantadine HCl has not been investigated. In the present study, the effects of amantadine HCl on cell growth, proliferation, and apoptosis in bovine cornea endothelial cells, and in vitro endothelial permeability were examined. Results showed that lower doses of amantadine HCl do not affect cell growth (≤ 20 μΜ), whereas higher doses of amantadine HCl inhibits cell growth (≥ 50 μΜ), induces apoptosis (2000 μΜ), increases sub-G1 phase growth arrest (2000 μΜ), causes DNA damage (≥ 1000 μΜ), and induces endothelial hyperpermeability (≥ 1000 μΜ) in bovine cornea endothelial cells; additionally, we also found that amantadine HCl attenuates the proliferation (≥ 200 μΜ) and arrests cell cycle at G1 phase (≥ 200 μΜ) in bovine cornea endothelial cells. In the present study, we measured the cytotoxic doses of amantadine HCl on cornea endothelial cells, which might be applied in evaluating the association of corneal edema.
format	article
author	Po-Yen Lee Yu-Hung Lai Po-Len Liu Ching-Chih Liu Chia-Cheng Su Fang-Yen Chiu Wei-Chung Cheng Shiuh-Liang Hsu Kai-Chun Cheng Li-Yi Chiu Tzu-En Kao Chia-Ching Lin Yo-Chen Chang Shu-Chi Wang Chia-Yang Li
author_facet	Po-Yen Lee Yu-Hung Lai Po-Len Liu Ching-Chih Liu Chia-Cheng Su Fang-Yen Chiu Wei-Chung Cheng Shiuh-Liang Hsu Kai-Chun Cheng Li-Yi Chiu Tzu-En Kao Chia-Ching Lin Yo-Chen Chang Shu-Chi Wang Chia-Yang Li
author_sort	Po-Yen Lee
title	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
title_short	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
title_full	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
title_fullStr	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
title_full_unstemmed	Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
title_sort	toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/4685db666c914a6b81d9725b14670eba
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Toxicity of amantadine hydrochloride on cultured bovine cornea endothelial cells

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