Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments

Abstract Bacteriophage infection of Lactococcus species can cause serious disruption of dairy fermentation processes. The most common isolates from the dairy environment are Siphoviridae lytic 936-type phages. To gain specific knowledge about this group of phages in Polish dairies, we examined 90 is...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	M. Chmielewska-Jeznach, J. K. Bardowski, A. K. Szczepankowska
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/468c079f7aa04ec88d53fe7a776037c3
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:468c079f7aa04ec88d53fe7a776037c3
record_format	dspace
spelling	oai:doaj.org-article:468c079f7aa04ec88d53fe7a776037c32021-12-02T15:08:54ZMolecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments10.1038/s41598-018-30371-32045-2322https://doaj.org/article/468c079f7aa04ec88d53fe7a776037c32018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30371-3https://doaj.org/toc/2045-2322Abstract Bacteriophage infection of Lactococcus species can cause serious disruption of dairy fermentation processes. The most common isolates from the dairy environment are Siphoviridae lytic 936-type phages. To gain specific knowledge about this group of phages in Polish dairies, we examined 90 isolates from 8 different locations. Based on restriction fragment length polymorphism analysis, coupled with physiological and molecular studies, the isolated phages were divided into 8 distinct groups. Whole-genome sequencing of single representatives from each phage group provided data about their biology and genetic composition. The phages present an overall conserved genome organization. High sequence homology to another Polish isolate, Lactococcus phage bIBB29, indicates their close phylogenetic relatedness to this strain. Such similarity may be suggestive of a general genome conservation among phages persisting in Polish dairies. Comparative genome analyses with other 936-type phages revealed several discriminative traits, including the presence and position of HNH endonuclease genes, varying number of orfs in the early gene region, and a putative TpeX gene. Interestingly, host range of the sequenced phages was restricted to L. lactis subsp. lactis biovar. diacetylactis strains. The results provide new data regarding phages present in the Polish dairy environment and permit analysis of their biology, genome composition and relatedness to other Lactococcus 936-type phages.M. Chmielewska-JeznachJ. K. BardowskiA. K. SzczepankowskaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-15 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q M. Chmielewska-Jeznach J. K. Bardowski A. K. Szczepankowska Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
description	Abstract Bacteriophage infection of Lactococcus species can cause serious disruption of dairy fermentation processes. The most common isolates from the dairy environment are Siphoviridae lytic 936-type phages. To gain specific knowledge about this group of phages in Polish dairies, we examined 90 isolates from 8 different locations. Based on restriction fragment length polymorphism analysis, coupled with physiological and molecular studies, the isolated phages were divided into 8 distinct groups. Whole-genome sequencing of single representatives from each phage group provided data about their biology and genetic composition. The phages present an overall conserved genome organization. High sequence homology to another Polish isolate, Lactococcus phage bIBB29, indicates their close phylogenetic relatedness to this strain. Such similarity may be suggestive of a general genome conservation among phages persisting in Polish dairies. Comparative genome analyses with other 936-type phages revealed several discriminative traits, including the presence and position of HNH endonuclease genes, varying number of orfs in the early gene region, and a putative TpeX gene. Interestingly, host range of the sequenced phages was restricted to L. lactis subsp. lactis biovar. diacetylactis strains. The results provide new data regarding phages present in the Polish dairy environment and permit analysis of their biology, genome composition and relatedness to other Lactococcus 936-type phages.
format	article
author	M. Chmielewska-Jeznach J. K. Bardowski A. K. Szczepankowska
author_facet	M. Chmielewska-Jeznach J. K. Bardowski A. K. Szczepankowska
author_sort	M. Chmielewska-Jeznach
title	Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
title_short	Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
title_full	Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
title_fullStr	Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
title_full_unstemmed	Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments
title_sort	molecular, physiological and phylogenetic traits of lactococcus 936-type phages from distinct dairy environments
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/468c079f7aa04ec88d53fe7a776037c3
work_keys_str_mv	AT mchmielewskajeznach molecularphysiologicalandphylogenetictraitsoflactococcus936typephagesfromdistinctdairyenvironments AT jkbardowski molecularphysiologicalandphylogenetictraitsoflactococcus936typephagesfromdistinctdairyenvironments AT akszczepankowska molecularphysiologicalandphylogenetictraitsoflactococcus936typephagesfromdistinctdairyenvironments
_version_	1718387994839220224

Molecular, physiological and phylogenetic traits of Lactococcus 936-type phages from distinct dairy environments

Ejemplares similares