Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns

Glycans are a major composition of the cell surface that interacts with the surrounding environment. The ability to carry out glycan-binding profile studies has been mainly done with glycan arrays. However, glycan arrays are not easily adaptable for cell surface and in vivo glycan recognition assays...

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Autores principales:	Theam Soon Lim, Karli Montague-Cardoso
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/46a78f673db449c4ba4f03bbc3594848
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spelling	oai:doaj.org-article:46a78f673db449c4ba4f03bbc35948482021-12-02T16:27:54ZGlycans housed by a bacteriophage enable rapid identification of glycan recognition patterns10.1038/s42003-021-02474-72399-3642https://doaj.org/article/46a78f673db449c4ba4f03bbc35948482021-08-01T00:00:00Zhttps://doi.org/10.1038/s42003-021-02474-7https://doaj.org/toc/2399-3642Glycans are a major composition of the cell surface that interacts with the surrounding environment. The ability to carry out glycan-binding profile studies has been mainly done with glycan arrays. However, glycan arrays are not easily adaptable for cell surface and in vivo glycan recognition assays. The Liquid Glycan Array (LiGA) reported recently by Sojitra et al. is an alternative glycan recognition assay that employs DNA barcoding, bioorthogonal ligation and deep sequencing technology. In LiGA, barcoded M13 virions are used to present glycans to allow rapid identification of binding partners based on sequence identity. This physical link between the glycan to the DNA sequence fitted in the phage genome provides an ingenious approach to maneuver glycan binding profile studies in various conditions.Theam Soon LimKarli Montague-CardosoNature PortfolioarticleBiology (General)QH301-705.5ENCommunications Biology, Vol 4, Iss 1, Pp 1-2 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biology (General) QH301-705.5
spellingShingle	Biology (General) QH301-705.5 Theam Soon Lim Karli Montague-Cardoso Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
description	Glycans are a major composition of the cell surface that interacts with the surrounding environment. The ability to carry out glycan-binding profile studies has been mainly done with glycan arrays. However, glycan arrays are not easily adaptable for cell surface and in vivo glycan recognition assays. The Liquid Glycan Array (LiGA) reported recently by Sojitra et al. is an alternative glycan recognition assay that employs DNA barcoding, bioorthogonal ligation and deep sequencing technology. In LiGA, barcoded M13 virions are used to present glycans to allow rapid identification of binding partners based on sequence identity. This physical link between the glycan to the DNA sequence fitted in the phage genome provides an ingenious approach to maneuver glycan binding profile studies in various conditions.
format	article
author	Theam Soon Lim Karli Montague-Cardoso
author_facet	Theam Soon Lim Karli Montague-Cardoso
author_sort	Theam Soon Lim
title	Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
title_short	Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
title_full	Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
title_fullStr	Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
title_full_unstemmed	Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
title_sort	glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/46a78f673db449c4ba4f03bbc3594848
work_keys_str_mv	AT theamsoonlim glycanshousedbyabacteriophageenablerapididentificationofglycanrecognitionpatterns AT karlimontaguecardoso glycanshousedbyabacteriophageenablerapididentificationofglycanrecognitionpatterns
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Glycans housed by a bacteriophage enable rapid identification of glycan recognition patterns

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