DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES

We investigated direct effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the surface properties and cytokine-producing activity of human monocytes/macrophages (Mc/Mphs). The CD14+ cells were isolated from peripheral blood of healthy donors by positive magnetic separation. The i...

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Autores principales:	N. D. Gazatova, M. E. Meniailo, V. V. Malashchenko, A. G. Goncharov, O. B. Melashchenko, E. M. Morozova, V. I. Seledtsov
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2019
Materias:	granulocyte-macrophage colony-stimulating factor monocyte/macrophages сd-expression cytokines Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/46a92220f2b642c68919e3a8ea2bf144
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spelling	oai:doaj.org-article:46a92220f2b642c68919e3a8ea2bf1442021-11-18T08:03:48ZDIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES1563-06252313-741X10.15789/1563-0625-2019-3-419-426https://doaj.org/article/46a92220f2b642c68919e3a8ea2bf1442019-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1815https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWe investigated direct effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the surface properties and cytokine-producing activity of human monocytes/macrophages (Mc/Mphs). The CD14+ cells were isolated from peripheral blood of healthy donors by positive magnetic separation. The isolated Mc/Mphs were cultured with lipopolysaccharide (LPS, 1 μg/ml) or without LPS for 24 hours. Membrane expression of CD14, CD16, CD119, CD124, and CD197 molecules was assessed by flow cytometry. The contents of tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β), IL-6 and IL-10 in culture supernatants were determined by the enzyme immunoassay technique. It was found that GM-CSF at a concentration range of 0.01-10 ng/ml did significantly reduce the number of cells expressing CD197 (CC receptor of chemokine 7), without significantly affecting the percentage of CD14+ (coreceptor of LPS), CD16+ (low-affinity Fc receptor), CD119+ (IFNγ receptor) and CD124+ (IL-4 receptor) cells. At the same time, GM-CSF reduced the contents of CD197+ macrophages, as well as CD14+, CD16+, and CD119+ cells among the activated cell population, without significantly altering the number of CD124+ cells. It was also shown that GM-CSF (10 ng/ml), was able to enhance production of TNFα and IL-6, but not IL-1β and IL-10 by activated Mc/Mphs. The results obtained indicate the ability of GM-CSF to exert both anti-inflammatory and pro-inflammatory effects upon macrophage cell populations. In general, such effects could contribute to the development of adaptive immunogenesis in peripheral tissues.N. D. GazatovaM. E. MeniailoV. V. MalashchenkoA. G. GoncharovO. B. MelashchenkoE. M. MorozovaV. I. SeledtsovSPb RAACIarticlegranulocyte-macrophage colony-stimulating factormonocyte/macrophagesсd-expressioncytokinesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 21, Iss 3, Pp 419-426 (2019)
institution	DOAJ
collection	DOAJ
language	RU
topic	granulocyte-macrophage colony-stimulating factor monocyte/macrophages сd-expression cytokines Immunologic diseases. Allergy RC581-607
spellingShingle	granulocyte-macrophage colony-stimulating factor monocyte/macrophages сd-expression cytokines Immunologic diseases. Allergy RC581-607 N. D. Gazatova M. E. Meniailo V. V. Malashchenko A. G. Goncharov O. B. Melashchenko E. M. Morozova V. I. Seledtsov DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
description	We investigated direct effects of granulocyte-macrophage colony-stimulating factor (GM-CSF) on the surface properties and cytokine-producing activity of human monocytes/macrophages (Mc/Mphs). The CD14+ cells were isolated from peripheral blood of healthy donors by positive magnetic separation. The isolated Mc/Mphs were cultured with lipopolysaccharide (LPS, 1 μg/ml) or without LPS for 24 hours. Membrane expression of CD14, CD16, CD119, CD124, and CD197 molecules was assessed by flow cytometry. The contents of tumor necrosis factor-α (TNFα), interleukin-1β (IL-1β), IL-6 and IL-10 in culture supernatants were determined by the enzyme immunoassay technique. It was found that GM-CSF at a concentration range of 0.01-10 ng/ml did significantly reduce the number of cells expressing CD197 (CC receptor of chemokine 7), without significantly affecting the percentage of CD14+ (coreceptor of LPS), CD16+ (low-affinity Fc receptor), CD119+ (IFNγ receptor) and CD124+ (IL-4 receptor) cells. At the same time, GM-CSF reduced the contents of CD197+ macrophages, as well as CD14+, CD16+, and CD119+ cells among the activated cell population, without significantly altering the number of CD124+ cells. It was also shown that GM-CSF (10 ng/ml), was able to enhance production of TNFα and IL-6, but not IL-1β and IL-10 by activated Mc/Mphs. The results obtained indicate the ability of GM-CSF to exert both anti-inflammatory and pro-inflammatory effects upon macrophage cell populations. In general, such effects could contribute to the development of adaptive immunogenesis in peripheral tissues.
format	article
author	N. D. Gazatova M. E. Meniailo V. V. Malashchenko A. G. Goncharov O. B. Melashchenko E. M. Morozova V. I. Seledtsov
author_facet	N. D. Gazatova M. E. Meniailo V. V. Malashchenko A. G. Goncharov O. B. Melashchenko E. M. Morozova V. I. Seledtsov
author_sort	N. D. Gazatova
title	DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
title_short	DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
title_full	DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
title_fullStr	DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
title_full_unstemmed	DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES
title_sort	direct effects of gm-csf on the functions of human monocytes/macrophages
publisher	SPb RAACI
publishDate	2019
url	https://doaj.org/article/46a92220f2b642c68919e3a8ea2bf144
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DIRECT EFFECTS OF GM-CSF ON THE FUNCTIONS OF HUMAN MONOCYTES/MACROPHAGES

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