Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry

Abstract Salivary polyamines are potential non-invasive tools for screening various types of cancers. For clinical use, the reproducibility of these metabolites should be evaluated under various storage conditions, including duration and temperature, to establish standard operating protocols. Polyam...

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Autores principales:	Atsumi Tomita, Masayo Mori, Kana Hiwatari, Eri Yamaguchi, Takao Itoi, Makoto Sunamura, Tomoyoshi Soga, Masaru Tomita, Masahiro Sugimoto
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46ac416dfa974aa08de987c568a8b91c
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spelling	oai:doaj.org-article:46ac416dfa974aa08de987c568a8b91c2021-12-02T11:41:04ZEffect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry10.1038/s41598-018-30482-x2045-2322https://doaj.org/article/46ac416dfa974aa08de987c568a8b91c2018-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-30482-xhttps://doaj.org/toc/2045-2322Abstract Salivary polyamines are potential non-invasive tools for screening various types of cancers. For clinical use, the reproducibility of these metabolites should be evaluated under various storage conditions, including duration and temperature, to establish standard operating protocols. Polyamines and amino acids in unstimulated whole saliva were quantified via liquid chromatography-mass spectrometry. Concentrations of time course samples were analysed after short-term storage for up to 240 min and long-term storage for up to 8 days under various storage conditions. As expected, storage at the lowest temperature (−18 °C) exerted the least pronounced effects on the quantified values in both tests. At a higher temperature, polyamines were more stable than amino acids, as evident from polyamine profiling. Addition of ethanol significantly stabilized polyamine profiles even at a higher temperature. Comparative processing of saliva revealed a minor effect of the solvent, whereas drying had a more prominent effect on polyamine profiles. Computational analyses evaluated the ability of polyamines to discriminate pancreatic cancer from controls. Repeated noise added tests were designed on the basis of the results of the storage tests; these analyses confirmed that the discriminative abilities were robust. These data contribute to the standardization of salivary storage conditions, thereby highlighting the clinical utility of saliva.Atsumi TomitaMasayo MoriKana HiwatariEri YamaguchiTakao ItoiMakoto SunamuraTomoyoshi SogaMasaru TomitaMasahiro SugimotoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Atsumi Tomita Masayo Mori Kana Hiwatari Eri Yamaguchi Takao Itoi Makoto Sunamura Tomoyoshi Soga Masaru Tomita Masahiro Sugimoto Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
description	Abstract Salivary polyamines are potential non-invasive tools for screening various types of cancers. For clinical use, the reproducibility of these metabolites should be evaluated under various storage conditions, including duration and temperature, to establish standard operating protocols. Polyamines and amino acids in unstimulated whole saliva were quantified via liquid chromatography-mass spectrometry. Concentrations of time course samples were analysed after short-term storage for up to 240 min and long-term storage for up to 8 days under various storage conditions. As expected, storage at the lowest temperature (−18 °C) exerted the least pronounced effects on the quantified values in both tests. At a higher temperature, polyamines were more stable than amino acids, as evident from polyamine profiling. Addition of ethanol significantly stabilized polyamine profiles even at a higher temperature. Comparative processing of saliva revealed a minor effect of the solvent, whereas drying had a more prominent effect on polyamine profiles. Computational analyses evaluated the ability of polyamines to discriminate pancreatic cancer from controls. Repeated noise added tests were designed on the basis of the results of the storage tests; these analyses confirmed that the discriminative abilities were robust. These data contribute to the standardization of salivary storage conditions, thereby highlighting the clinical utility of saliva.
format	article
author	Atsumi Tomita Masayo Mori Kana Hiwatari Eri Yamaguchi Takao Itoi Makoto Sunamura Tomoyoshi Soga Masaru Tomita Masahiro Sugimoto
author_facet	Atsumi Tomita Masayo Mori Kana Hiwatari Eri Yamaguchi Takao Itoi Makoto Sunamura Tomoyoshi Soga Masaru Tomita Masahiro Sugimoto
author_sort	Atsumi Tomita
title	Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
title_short	Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
title_full	Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
title_fullStr	Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
title_full_unstemmed	Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
title_sort	effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/46ac416dfa974aa08de987c568a8b91c
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Effect of storage conditions on salivary polyamines quantified via liquid chromatography-mass spectrometry

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