Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

Abstract The enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the...

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Autores principales:	Iuliia Golovynska, Tatiana V. Beregova, Tatiana M. Falalyeyeva, Ludmila I. Stepanova, Sergii Golovynskyi, Junle Qu, Tymish Y. Ohulchanskyy
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46b02782b43548abb03224ad7d73365e
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spelling	oai:doaj.org-article:46b02782b43548abb03224ad7d73365e2021-12-02T12:32:48ZPeripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies10.1038/s41598-018-25753-62045-2322https://doaj.org/article/46b02782b43548abb03224ad7d73365e2018-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-018-25753-6https://doaj.org/toc/2045-2322Abstract The enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the present study, we clarified the types of stomach cells that express the NMDARs that are involved in GAS regulation. The pharmacological method of isolated stomach perfusion by Ghosh and Shild combined with direct mapping of NMDARs by fluorescence microscopy in the rat stomach was employed. By immunofluorescence labeling with an anti-NMDA-NR1 antibody, NMDARs were found to be highly expressed in nerve cells of the submucosal and myenteric plexuses in the stomach. The exact localization of the NMDARs relevant to GAS and its mechanism of action were determined by stimulating different receptors of neuronal and stomach cells using specific secretagogues for NMDA and by selectively blocking those receptors. NMDARs relevant to GAS stimulation are mainly localized in cholinergic interneurons; however, all of the nerve cells of the submucosal ganglia are involved in the stimulating process. In addition, the NMDARs in parietal cells are involved in gastric acid inhibition via influencing H2-histamine receptors.Iuliia GolovynskaTatiana V. BeregovaTatiana M. FalalyeyevaLudmila I. StepanovaSergii GolovynskyiJunle QuTymish Y. OhulchanskyyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 8, Iss 1, Pp 1-11 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Iuliia Golovynska Tatiana V. Beregova Tatiana M. Falalyeyeva Ludmila I. Stepanova Sergii Golovynskyi Junle Qu Tymish Y. Ohulchanskyy Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
description	Abstract The enteric nervous system (ENS) and a glutamate receptor (GluR), N-methyl-D-aspartate receptor (NMDAR), participate in gastric acid secretion (GAS) regulation. NMDARs are localized in different stomach cells; however, knowledge of NMDAR expression and function in the ENS is limited. In the present study, we clarified the types of stomach cells that express the NMDARs that are involved in GAS regulation. The pharmacological method of isolated stomach perfusion by Ghosh and Shild combined with direct mapping of NMDARs by fluorescence microscopy in the rat stomach was employed. By immunofluorescence labeling with an anti-NMDA-NR1 antibody, NMDARs were found to be highly expressed in nerve cells of the submucosal and myenteric plexuses in the stomach. The exact localization of the NMDARs relevant to GAS and its mechanism of action were determined by stimulating different receptors of neuronal and stomach cells using specific secretagogues for NMDA and by selectively blocking those receptors. NMDARs relevant to GAS stimulation are mainly localized in cholinergic interneurons; however, all of the nerve cells of the submucosal ganglia are involved in the stimulating process. In addition, the NMDARs in parietal cells are involved in gastric acid inhibition via influencing H2-histamine receptors.
format	article
author	Iuliia Golovynska Tatiana V. Beregova Tatiana M. Falalyeyeva Ludmila I. Stepanova Sergii Golovynskyi Junle Qu Tymish Y. Ohulchanskyy
author_facet	Iuliia Golovynska Tatiana V. Beregova Tatiana M. Falalyeyeva Ludmila I. Stepanova Sergii Golovynskyi Junle Qu Tymish Y. Ohulchanskyy
author_sort	Iuliia Golovynska
title	Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
title_short	Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
title_full	Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
title_fullStr	Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
title_full_unstemmed	Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
title_sort	peripheral n-methyl-d-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/46b02782b43548abb03224ad7d73365e
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Peripheral N-methyl-D-aspartate receptor localization and role in gastric acid secretion regulation: immunofluorescence and pharmacological studies

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