PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES

Abstract. TLR4 is a key player in signaling system of host cells. Possible role of TLR4 is actively discussed, e.g. its significance for phagocytosis. A capacity of neutrophils to engulf FITC-labeled E. coli bacteria upon activation with LPS of different origin was studied in presence of anti-TLR4 M...

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Autores principales:	S. V. Zubova, S. V. Grachev, I. R. Prokhorenko
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2014
Materias:	tlr4 endotoxins lipopolysaccharides phagosytosis neutrophils Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/46b559967c41409e905f58b6c152ac6c
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spelling	oai:doaj.org-article:46b559967c41409e905f58b6c152ac6c2021-11-18T08:03:40ZPARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES1563-06252313-741X10.15789/1563-0625-2012-3-219-222https://doaj.org/article/46b559967c41409e905f58b6c152ac6c2014-07-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/354https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XAbstract. TLR4 is a key player in signaling system of host cells. Possible role of TLR4 is actively discussed, e.g. its significance for phagocytosis. A capacity of neutrophils to engulf FITC-labeled E. coli bacteria upon activation with LPS of different origin was studied in presence of anti-TLR4 Mab’s (HTA125 clone). It was shown that, in whole blood, TLR4 does not play any essential role in engulfment of bacteria by the neutrophils. Phagocytic activity of neutrophils in blood increases increased after their priming with E. coli endotoxins. LPS from Rb. сapsulatus did not affect phagocytosis. In presence of endotoxins, the degree of TLR4 involvement in neutrophil phagocytosis depends on LPS structure.S. V. ZubovaS. V. GrachevI. R. ProkhorenkoSPb RAACIarticletlr4endotoxinslipopolysaccharidesphagosytosisneutrophilsImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 14, Iss 3, Pp 219-222 (2014)
institution	DOAJ
collection	DOAJ
language	RU
topic	tlr4 endotoxins lipopolysaccharides phagosytosis neutrophils Immunologic diseases. Allergy RC581-607
spellingShingle	tlr4 endotoxins lipopolysaccharides phagosytosis neutrophils Immunologic diseases. Allergy RC581-607 S. V. Zubova S. V. Grachev I. R. Prokhorenko PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
description	Abstract. TLR4 is a key player in signaling system of host cells. Possible role of TLR4 is actively discussed, e.g. its significance for phagocytosis. A capacity of neutrophils to engulf FITC-labeled E. coli bacteria upon activation with LPS of different origin was studied in presence of anti-TLR4 Mab’s (HTA125 clone). It was shown that, in whole blood, TLR4 does not play any essential role in engulfment of bacteria by the neutrophils. Phagocytic activity of neutrophils in blood increases increased after their priming with E. coli endotoxins. LPS from Rb. сapsulatus did not affect phagocytosis. In presence of endotoxins, the degree of TLR4 involvement in neutrophil phagocytosis depends on LPS structure.
format	article
author	S. V. Zubova S. V. Grachev I. R. Prokhorenko
author_facet	S. V. Zubova S. V. Grachev I. R. Prokhorenko
author_sort	S. V. Zubova
title	PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
title_short	PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
title_full	PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
title_fullStr	PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
title_full_unstemmed	PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES
title_sort	participation of tlr4 in engulfment of escherichia coli by human blood neutrophils in presence of lipopolysaccharides
publisher	SPb RAACI
publishDate	2014
url	https://doaj.org/article/46b559967c41409e905f58b6c152ac6c
work_keys_str_mv	AT svzubova participationoftlr4inengulfmentofescherichiacolibyhumanbloodneutrophilsinpresenceoflipopolysaccharides AT svgrachev participationoftlr4inengulfmentofescherichiacolibyhumanbloodneutrophilsinpresenceoflipopolysaccharides AT irprokhorenko participationoftlr4inengulfmentofescherichiacolibyhumanbloodneutrophilsinpresenceoflipopolysaccharides
_version_	1718422474116300800

PARTICIPATION OF TLR4 IN ENGULFMENT OF ESCHERICHIA COLI BY HUMAN BLOOD NEUTROPHILS IN PRESENCE OF LIPOPOLYSACCHARIDES

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