Effects of copolymer component on the properties of phosphorylcholine micelles

Zhengzhong Wu,1 Mengtan Cai,1 Jun Cao,2 Jiaxing Zhang,1 Xianglin Luo1,3 1College of Polymer Science and Engineering, 2National Engineering Research Center for Biomaterials, 3State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, People’s Republic of China Abs...

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Autores principales:	Wu Z, Cai M, Cao J, Zhang J, Luo X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	zwitterionic reduction-sensitive disulfide phosphorylcholine Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/46bf1aec215d420e80cd6114c6badc57
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spelling	oai:doaj.org-article:46bf1aec215d420e80cd6114c6badc572021-12-02T02:41:35ZEffects of copolymer component on the properties of phosphorylcholine micelles1178-2013https://doaj.org/article/46bf1aec215d420e80cd6114c6badc572017-01-01T00:00:00Zhttps://www.dovepress.com/effects-of-copolymer-component-on-the-properties-of-phosphorylcholine--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhengzhong Wu,1 Mengtan Cai,1 Jun Cao,2 Jiaxing Zhang,1 Xianglin Luo1,3 1College of Polymer Science and Engineering, 2National Engineering Research Center for Biomaterials, 3State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, People’s Republic of China Abstract: Zwitterionic polymers have unique features, such as good compatibility, and show promise in the application of drug delivery. In this study, the zwitterionic copolymers, poly(ε-caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) with disulfide (PCL-ss-PMPC) or poly(ε-caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) or without disulfide (PCL-PMPC) and with different block lengths in PCL-ss-PMPC, were designed. The designed copolymers were obtained by a combination of ring-opening polymerization and atom transferring radical polymerization. The crystallization properties of these polymers were investigated. The micelles were prepared based on the obtained copolymers with zwitterionic phosphorylcholine as the hydrophilic shell and PCL as the hydrophobic core. The size distributions of the blank micelles and the doxorubicin (DOX)-loaded micelles were uniform, and the micelle diameters were <100 nm. In vitro drug release and intracellular drug release results showed that DOX-loaded PCL-ss-PMPC micelles could release drugs faster responding to the reduction condition and the intracellular microenvironment in contrast to PCL-PMPC micelles. Moreover, in vitro cytotoxicity evaluation revealed that the designed copolymers possessed low cell toxicity, and the inhibiting effect of DOX-loaded phosphorylcholine micelles to tumor cells was related to the components of these copolymers. These results reveal that the reduction-responsive phosphorylcholine micelles with a suitable ratio of hydrophilic/hydrophobic units can serve as promising drug carriers. Keywords: zwitterionic, reduction-sensitive, disulfide, phosphorylcholineWu ZCai MCao JZhang JLuo XDove Medical Pressarticlezwitterionicreduction-sensitivedisulfidephosphorylcholineMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 487-500 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	zwitterionic reduction-sensitive disulfide phosphorylcholine Medicine (General) R5-920
spellingShingle	zwitterionic reduction-sensitive disulfide phosphorylcholine Medicine (General) R5-920 Wu Z Cai M Cao J Zhang J Luo X Effects of copolymer component on the properties of phosphorylcholine micelles
description	Zhengzhong Wu,1 Mengtan Cai,1 Jun Cao,2 Jiaxing Zhang,1 Xianglin Luo1,3 1College of Polymer Science and Engineering, 2National Engineering Research Center for Biomaterials, 3State Key Laboratory of Polymer Materials Engineering, Sichuan University, Chengdu, People’s Republic of China Abstract: Zwitterionic polymers have unique features, such as good compatibility, and show promise in the application of drug delivery. In this study, the zwitterionic copolymers, poly(ε-caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) with disulfide (PCL-ss-PMPC) or poly(ε-caprolactone)-b-poly(2-methacryloyloxyethyl phosphorylcholine) or without disulfide (PCL-PMPC) and with different block lengths in PCL-ss-PMPC, were designed. The designed copolymers were obtained by a combination of ring-opening polymerization and atom transferring radical polymerization. The crystallization properties of these polymers were investigated. The micelles were prepared based on the obtained copolymers with zwitterionic phosphorylcholine as the hydrophilic shell and PCL as the hydrophobic core. The size distributions of the blank micelles and the doxorubicin (DOX)-loaded micelles were uniform, and the micelle diameters were <100 nm. In vitro drug release and intracellular drug release results showed that DOX-loaded PCL-ss-PMPC micelles could release drugs faster responding to the reduction condition and the intracellular microenvironment in contrast to PCL-PMPC micelles. Moreover, in vitro cytotoxicity evaluation revealed that the designed copolymers possessed low cell toxicity, and the inhibiting effect of DOX-loaded phosphorylcholine micelles to tumor cells was related to the components of these copolymers. These results reveal that the reduction-responsive phosphorylcholine micelles with a suitable ratio of hydrophilic/hydrophobic units can serve as promising drug carriers. Keywords: zwitterionic, reduction-sensitive, disulfide, phosphorylcholine
format	article
author	Wu Z Cai M Cao J Zhang J Luo X
author_facet	Wu Z Cai M Cao J Zhang J Luo X
author_sort	Wu Z
title	Effects of copolymer component on the properties of phosphorylcholine micelles
title_short	Effects of copolymer component on the properties of phosphorylcholine micelles
title_full	Effects of copolymer component on the properties of phosphorylcholine micelles
title_fullStr	Effects of copolymer component on the properties of phosphorylcholine micelles
title_full_unstemmed	Effects of copolymer component on the properties of phosphorylcholine micelles
title_sort	effects of copolymer component on the properties of phosphorylcholine micelles
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/46bf1aec215d420e80cd6114c6badc57
work_keys_str_mv	AT wuz effectsofcopolymercomponentonthepropertiesofphosphorylcholinemicelles AT caim effectsofcopolymercomponentonthepropertiesofphosphorylcholinemicelles AT caoj effectsofcopolymercomponentonthepropertiesofphosphorylcholinemicelles AT zhangj effectsofcopolymercomponentonthepropertiesofphosphorylcholinemicelles AT luox effectsofcopolymercomponentonthepropertiesofphosphorylcholinemicelles
_version_	1718402281202778112

Effects of copolymer component on the properties of phosphorylcholine micelles

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