Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation

Fruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits...

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Autores principales:	Xinxin Feng, Xiaofeng Liu, Yuxiu Jia, Huifang Liu, Liulin Li
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	‘Suli’ pears hardened fruit lignin biosynthesis transcriptomic analysis Plant culture SB1-1110
Acceso en línea:	https://doaj.org/article/46c3c9d491d44c4fa4224bdcdc6e710f
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spelling	oai:doaj.org-article:46c3c9d491d44c4fa4224bdcdc6e710f2021-11-25T17:47:25ZTranscriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation10.3390/horticulturae71104672311-7524https://doaj.org/article/46c3c9d491d44c4fa4224bdcdc6e710f2021-11-01T00:00:00Zhttps://www.mdpi.com/2311-7524/7/11/467https://doaj.org/toc/2311-7524Fruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits (HFs) of ‘Suli’ pear (<i>Pyrus bretschneideri</i> Rehd) was used to examine the molecular mechanism of fruit hardening. We found that hardened fruits (HFs), especially those in the top region within 35–180 days after flowering (DAF), possessed a prominently higher lignin level than normal fruits (NFs). Subsequently, transcriptome sequencing of fruit at 35 DAF identified 4391 [HF shoulder region (HF_S) vs. NF top region (NF_T)], 3849 (HF_T vs. NF_T) and 408 (HF_T vs. HF_S) differentially expressed genes. Among them, we clarified 26 unigenes that encode 10 enzymes as candidate genes participating in lignin biosynthesis. Thus, high expression of the lignin biosynthetic gene impels lignin to accumulate in HFs. Some transcription factors were evaluated to link with lignin formation and subsequently analysed. In addition, the genes participating in flavonoid/proanthocyanidin biosynthesis dropped from the HF_T to the HF_S, suggesting that the flavonoid metabolic pathway was reduced to promote intermediate metabolites to be converted to the lignin biosynthetic pathway. To conclude, this study lays a solid theoretical foundation and provides reference data for investigating the mechanism by which lignin accumulates in hardened pear fruit.Xinxin FengXiaofeng LiuYuxiu JiaHuifang LiuLiulin LiMDPI AGarticle‘Suli’ pearshardened fruitlignin biosynthesistranscriptomic analysisPlant cultureSB1-1110ENHorticulturae, Vol 7, Iss 467, p 467 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	‘Suli’ pears hardened fruit lignin biosynthesis transcriptomic analysis Plant culture SB1-1110
spellingShingle	‘Suli’ pears hardened fruit lignin biosynthesis transcriptomic analysis Plant culture SB1-1110 Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
description	Fruit hardening, one of the physiological disorders influencing the quality of pear, is usually accompanied by fruit lignification. Nonetheless, the mechanism by which lignin accumulates in hardened pear fruit is still unknown. Here, Transcriptome analysis of normal fruits (NFs) and hardened fruits (HFs) of ‘Suli’ pear (<i>Pyrus bretschneideri</i> Rehd) was used to examine the molecular mechanism of fruit hardening. We found that hardened fruits (HFs), especially those in the top region within 35–180 days after flowering (DAF), possessed a prominently higher lignin level than normal fruits (NFs). Subsequently, transcriptome sequencing of fruit at 35 DAF identified 4391 [HF shoulder region (HF_S) vs. NF top region (NF_T)], 3849 (HF_T vs. NF_T) and 408 (HF_T vs. HF_S) differentially expressed genes. Among them, we clarified 26 unigenes that encode 10 enzymes as candidate genes participating in lignin biosynthesis. Thus, high expression of the lignin biosynthetic gene impels lignin to accumulate in HFs. Some transcription factors were evaluated to link with lignin formation and subsequently analysed. In addition, the genes participating in flavonoid/proanthocyanidin biosynthesis dropped from the HF_T to the HF_S, suggesting that the flavonoid metabolic pathway was reduced to promote intermediate metabolites to be converted to the lignin biosynthetic pathway. To conclude, this study lays a solid theoretical foundation and provides reference data for investigating the mechanism by which lignin accumulates in hardened pear fruit.
format	article
author	Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li
author_facet	Xinxin Feng Xiaofeng Liu Yuxiu Jia Huifang Liu Liulin Li
author_sort	Xinxin Feng
title	Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
title_short	Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
title_full	Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
title_fullStr	Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
title_full_unstemmed	Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation
title_sort	transcriptomic analysis of hardened ‘suli’ pear (<i>pyrus bretschneideri</i> rehd) for identification of key genes for lignin biosynthesis and accumulation
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/46c3c9d491d44c4fa4224bdcdc6e710f
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Transcriptomic Analysis of Hardened ‘Suli’ Pear (<i>Pyrus bretschneideri</i> Rehd) for Identification of Key Genes for Lignin Biosynthesis and Accumulation

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