TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy

Abstract Optimization of sample, imaging and data processing parameters is an essential task in localization based super-resolution microscopy, where the final image quality strongly depends on the imaging of single isolated fluorescent molecules. A computational solution that uses a simulator softw...

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Autores principales:	Tibor Novák, Tamás Gajdos, József Sinkó, Gábor Szabó, Miklós Erdélyi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46c50a8a01ce43aa8b307f318c72a9e8
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spelling	oai:doaj.org-article:46c50a8a01ce43aa8b307f318c72a9e82021-12-02T15:05:32ZTestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy10.1038/s41598-017-01122-72045-2322https://doaj.org/article/46c50a8a01ce43aa8b307f318c72a9e82017-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01122-7https://doaj.org/toc/2045-2322Abstract Optimization of sample, imaging and data processing parameters is an essential task in localization based super-resolution microscopy, where the final image quality strongly depends on the imaging of single isolated fluorescent molecules. A computational solution that uses a simulator software for the generation of test data stacks was proposed, developed and tested. The implemented advanced physical models such as scalar and vector based point spread functions, polarization sensitive detection, drift, spectral crosstalk, structured background etc., made the simulation results more realistic and helped us interpret the final super-resolved images and distinguish between real structures and imaging artefacts.Tibor NovákTamás GajdosJózsef SinkóGábor SzabóMiklós ErdélyiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tibor Novák Tamás Gajdos József Sinkó Gábor Szabó Miklós Erdélyi TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
description	Abstract Optimization of sample, imaging and data processing parameters is an essential task in localization based super-resolution microscopy, where the final image quality strongly depends on the imaging of single isolated fluorescent molecules. A computational solution that uses a simulator software for the generation of test data stacks was proposed, developed and tested. The implemented advanced physical models such as scalar and vector based point spread functions, polarization sensitive detection, drift, spectral crosstalk, structured background etc., made the simulation results more realistic and helped us interpret the final super-resolved images and distinguish between real structures and imaging artefacts.
format	article
author	Tibor Novák Tamás Gajdos József Sinkó Gábor Szabó Miklós Erdélyi
author_facet	Tibor Novák Tamás Gajdos József Sinkó Gábor Szabó Miklós Erdélyi
author_sort	Tibor Novák
title	TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
title_short	TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
title_full	TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
title_fullStr	TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
title_full_unstemmed	TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy
title_sort	teststorm: versatile simulator software for multimodal super-resolution localization fluorescence microscopy
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/46c50a8a01ce43aa8b307f318c72a9e8
work_keys_str_mv	AT tibornovak teststormversatilesimulatorsoftwareformultimodalsuperresolutionlocalizationfluorescencemicroscopy AT tamasgajdos teststormversatilesimulatorsoftwareformultimodalsuperresolutionlocalizationfluorescencemicroscopy AT jozsefsinko teststormversatilesimulatorsoftwareformultimodalsuperresolutionlocalizationfluorescencemicroscopy AT gaborszabo teststormversatilesimulatorsoftwareformultimodalsuperresolutionlocalizationfluorescencemicroscopy AT mikloserdelyi teststormversatilesimulatorsoftwareformultimodalsuperresolutionlocalizationfluorescencemicroscopy
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TestSTORM: Versatile simulator software for multimodal super-resolution localization fluorescence microscopy

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