Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell

Abstract Maintaining functional stability of microbial electrolysis cell (MEC) treating wastewater depends on maintaining functional redundancy of efficient electroactive bacteria (EAB) on the anode biofilm. Therefore, investigating whether efficient EAB competing for the same resources (electron do...

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Autores principales: Veerraghavulu Sapireddy, Krishna P. Katuri, Ali Muhammad, Pascal E. Saikaly
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Publicado: Nature Portfolio 2021
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spelling oai:doaj.org-article:46c6dde8cf934e37894ca10b07ad59922021-12-02T17:51:14ZCompetition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell10.1038/s41522-021-00218-32055-5008https://doaj.org/article/46c6dde8cf934e37894ca10b07ad59922021-05-01T00:00:00Zhttps://doi.org/10.1038/s41522-021-00218-3https://doaj.org/toc/2055-5008Abstract Maintaining functional stability of microbial electrolysis cell (MEC) treating wastewater depends on maintaining functional redundancy of efficient electroactive bacteria (EAB) on the anode biofilm. Therefore, investigating whether efficient EAB competing for the same resources (electron donor and acceptor) co-exist at the anode biofilm is key for the successful application of MEC for wastewater treatment. Here, we compare the electrochemical and kinetic properties of two efficient acetoclastic EAB, Geobacter sulfurreducens (GS) and Desulfuromonas acetexigens (DA), grown as monoculture in MECs fed with acetate. Additionally, we monitor the evolution of DA and GS in co-culture MECs fed with acetate or domestic wastewater using fluorescent in situ hybridization. The apparent Monod kinetic parameters reveal that DA possesses higher j max (10.7 ± 0.4 A/m2) and lower K S, app (2 ± 0.15 mM) compared to GS biofilms (j max: 9.6 ± 0.2 A/m2 and K S, app: 2.9 ± 0.2 mM). Further, more donor electrons are diverted to the anode for respiration in DA compared to GS. In acetate-fed co-culture MECs, DA (98% abundance) outcompete GS for anode-dependent growth. In contrast, both EAB co-exist (DA: 55 ± 2%; GS: 24 ± 1.1%) in wastewater-fed co-culture MECs despite the advantage of DA over GS based on kinetic parameters alone. The co-existence of efficient acetoclastic EAB with high current density in MECs fed with wastewater is significant in the context of functional redundancy to maintain stable performance. Our findings also provide insight to future studies on bioaugmentation of wastewater-fed MECs with efficient EAB to enhance performance.Veerraghavulu SapireddyKrishna P. KaturiAli MuhammadPascal E. SaikalyNature PortfolioarticleMicrobial ecologyQR100-130ENnpj Biofilms and Microbiomes, Vol 7, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Microbial ecology
QR100-130
spellingShingle Microbial ecology
QR100-130
Veerraghavulu Sapireddy
Krishna P. Katuri
Ali Muhammad
Pascal E. Saikaly
Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
description Abstract Maintaining functional stability of microbial electrolysis cell (MEC) treating wastewater depends on maintaining functional redundancy of efficient electroactive bacteria (EAB) on the anode biofilm. Therefore, investigating whether efficient EAB competing for the same resources (electron donor and acceptor) co-exist at the anode biofilm is key for the successful application of MEC for wastewater treatment. Here, we compare the electrochemical and kinetic properties of two efficient acetoclastic EAB, Geobacter sulfurreducens (GS) and Desulfuromonas acetexigens (DA), grown as monoculture in MECs fed with acetate. Additionally, we monitor the evolution of DA and GS in co-culture MECs fed with acetate or domestic wastewater using fluorescent in situ hybridization. The apparent Monod kinetic parameters reveal that DA possesses higher j max (10.7 ± 0.4 A/m2) and lower K S, app (2 ± 0.15 mM) compared to GS biofilms (j max: 9.6 ± 0.2 A/m2 and K S, app: 2.9 ± 0.2 mM). Further, more donor electrons are diverted to the anode for respiration in DA compared to GS. In acetate-fed co-culture MECs, DA (98% abundance) outcompete GS for anode-dependent growth. In contrast, both EAB co-exist (DA: 55 ± 2%; GS: 24 ± 1.1%) in wastewater-fed co-culture MECs despite the advantage of DA over GS based on kinetic parameters alone. The co-existence of efficient acetoclastic EAB with high current density in MECs fed with wastewater is significant in the context of functional redundancy to maintain stable performance. Our findings also provide insight to future studies on bioaugmentation of wastewater-fed MECs with efficient EAB to enhance performance.
format article
author Veerraghavulu Sapireddy
Krishna P. Katuri
Ali Muhammad
Pascal E. Saikaly
author_facet Veerraghavulu Sapireddy
Krishna P. Katuri
Ali Muhammad
Pascal E. Saikaly
author_sort Veerraghavulu Sapireddy
title Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
title_short Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
title_full Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
title_fullStr Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
title_full_unstemmed Competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
title_sort competition of two highly specialized and efficient acetoclastic electroactive bacteria for acetate in biofilm anode of microbial electrolysis cell
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/46c6dde8cf934e37894ca10b07ad5992
work_keys_str_mv AT veerraghavulusapireddy competitionoftwohighlyspecializedandefficientacetoclasticelectroactivebacteriaforacetateinbiofilmanodeofmicrobialelectrolysiscell
AT krishnapkaturi competitionoftwohighlyspecializedandefficientacetoclasticelectroactivebacteriaforacetateinbiofilmanodeofmicrobialelectrolysiscell
AT alimuhammad competitionoftwohighlyspecializedandefficientacetoclasticelectroactivebacteriaforacetateinbiofilmanodeofmicrobialelectrolysiscell
AT pascalesaikaly competitionoftwohighlyspecializedandefficientacetoclasticelectroactivebacteriaforacetateinbiofilmanodeofmicrobialelectrolysiscell
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