Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Abstract Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of differ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Adrian Elter, Tina Bock, Dieter Spiehl, Giulio Russo, Steffen C. Hinz, Sebastian Bitsch, Eva Baum, Markus Langhans, Tobias Meckel, Edgar Dörsam, Harald Kolmar, Gerhard Schwall
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46cba929f3f3461aa0a629fdd305a1ef
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:46cba929f3f3461aa0a629fdd305a1ef
record_format	dspace
spelling	oai:doaj.org-article:46cba929f3f3461aa0a629fdd305a1ef2021-12-02T14:26:16ZCarbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays10.1038/s41598-021-87072-72045-2322https://doaj.org/article/46cba929f3f3461aa0a629fdd305a1ef2021-04-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-87072-7https://doaj.org/toc/2045-2322Abstract Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.Adrian ElterTina BockDieter SpiehlGiulio RussoSteffen C. HinzSebastian BitschEva BaumMarkus LanghansTobias MeckelEdgar DörsamHarald KolmarGerhard SchwallNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Adrian Elter Tina Bock Dieter Spiehl Giulio Russo Steffen C. Hinz Sebastian Bitsch Eva Baum Markus Langhans Tobias Meckel Edgar Dörsam Harald Kolmar Gerhard Schwall Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
description	Abstract Since the pandemic outbreak of Covid-19 in December 2019, several lateral flow assay (LFA) devices were developed to enable the constant monitoring of regional and global infection processes. Additionally, innumerable lateral flow test devices are frequently used for determination of different clinical parameters, food safety, and environmental factors. Since common LFAs rely on non-biodegradable nitrocellulose membranes, we focused on their replacement by cellulose-composed, biodegradable papers. We report the development of cellulose paper-based lateral flow immunoassays using a carbohydrate-binding module-fused to detection antibodies. Studies regarding the protein binding capacity and potential protein wash-off effects on cellulose paper demonstrated a 2.7-fold protein binding capacity of CBM-fused antibody fragments compared to the sole antibody fragment. Furthermore, this strategy improved the spatial retention of CBM-fused detection antibodies to the test area, which resulted in an enhanced sensitivity and improved overall LFA-performance compared to the naked detection antibody. CBM-assisted antibodies were validated by implementation into two model lateral flow test devices (pregnancy detection and the detection of SARS-CoV-2 specific antibodies). The CBM-assisted pregnancy LFA demonstrated sensitive detection of human gonadotropin (hCG) in synthetic urine and the CBM-assisted Covid-19 antibody LFA was able to detect SARS-CoV-2 specific antibodies present in serum. Our findings pave the way to the more frequent use of cellulose-based papers instead of nitrocellulose in LFA devices and thus potentially improve the sustainability in the field of POC diagnostics.
format	article
author	Adrian Elter Tina Bock Dieter Spiehl Giulio Russo Steffen C. Hinz Sebastian Bitsch Eva Baum Markus Langhans Tobias Meckel Edgar Dörsam Harald Kolmar Gerhard Schwall
author_facet	Adrian Elter Tina Bock Dieter Spiehl Giulio Russo Steffen C. Hinz Sebastian Bitsch Eva Baum Markus Langhans Tobias Meckel Edgar Dörsam Harald Kolmar Gerhard Schwall
author_sort	Adrian Elter
title	Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
title_short	Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
title_full	Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
title_fullStr	Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
title_full_unstemmed	Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
title_sort	carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/46cba929f3f3461aa0a629fdd305a1ef
work_keys_str_mv	AT adrianelter carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT tinabock carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT dieterspiehl carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT giuliorusso carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT steffenchinz carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT sebastianbitsch carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT evabaum carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT markuslanghans carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT tobiasmeckel carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT edgardorsam carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT haraldkolmar carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays AT gerhardschwall carbohydratebindingmodulefusedantibodiesimprovetheperformanceofcellulosebasedlateralflowimmunoassays
_version_	1718391364857626624

Carbohydrate binding module-fused antibodies improve the performance of cellulose-based lateral flow immunoassays

Ejemplares similares