Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis

Christian M Rodríguez-Razón,1 Irinea Yañez-Sánchez,2 Vicente O Ramos-Santillan,1 Celso Velásquez-Ordóñez,2 Susan A Gutiérrez-Rubio,1 Maritza R García-García,3 Roció I L&oacute...

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Autores principales:	Rodríguez-Razón CM, Yañez-Sánchez I, Ramos-Santillan VO, Velásquez-Ordóñez C, Gutiérrez-Rubio SA, García-García MR, López-Roa RI, Sánchez-Hernández PE, Daneri-Navarro A, García-Iglesias T
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	Silver nanoparticles breast cancer adhesion apoptosis and transcriptome. Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/46dbc3e583fb485fbb0858475002b893
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spelling	oai:doaj.org-article:46dbc3e583fb485fbb0858475002b8932021-12-02T05:09:48ZAdhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis1178-2013https://doaj.org/article/46dbc3e583fb485fbb0858475002b8932018-02-01T00:00:00Zhttps://www.dovepress.com/adhesion-proliferation-and-apoptosis-in-different-molecular-portraits--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Christian M Rodríguez-Razón,1 Irinea Yañez-Sánchez,2 Vicente O Ramos-Santillan,1 Celso Velásquez-Ordóñez,2 Susan A Gutiérrez-Rubio,1 Maritza R García-García,3 Roció I López-Roa,1 Pedro E Sánchez-Hernández,1 Adrian Daneri-Navarro,1 Trinidad García-Iglesias1 1Laboratory of Immunology and Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Center of Health Sciences, University of Guadalajara, Jalisco, Mexico; 2Center for Research in Nanosciences and Nanotechnology, Department of Natural and Exact Sciences, University Center of the Valleys, University of Guadalajara, Jalisco, Mexico; 3Department of Health Sciences, University Center of the High University of Guadalajara, Jalisco, Mexico Background: Silver nanoparticles (AgNPs) have attracted considerable attention due to the variety of their applications in medicine and other sciences. AgNPs have been used in vitro for treatment of various diseases, such as hepatitis B and herpes simplex infections as well as colon, cervical, and lung cancers. In this study, we assessed the effect on proliferation, adhesion, and apoptosis in breast cancer cell lines of different molecular profiles (MCF7, HCC1954, and HCC70) exposed to AgNPs (2–9 nm).Methods: Breast cancer cell lines were incubated in vitro; MTT assay was used to assess proliferation. Adhesion was determined by real-time analysis with the xCELLingence system. Propidium iodide and fluorescein isothiocyanate-Annexin V assay were used to measure apoptosis. The transcriptome was assessed by gene expression microarray and Probabilistic Graphical Model (PGM) analyses.Results: The results showed a decreased adhesion in breast cancer cell lines and the control exposed to AgNPs was noted in 24 hours (p≤0.05). We observed a significant reduction in the proliferation of MCF7 and HCC70, but not in HCC1954. Apoptotic activity was seen in all cell lines exposed to AgNPs, with an apoptosis percentage of more than 60% in cancer cell lines and less than 60% in the control. PGM analysis confirmed, to some extent, the effects of AgNPs primarily on adhesion by changes in the extracellular matrix.Conclusion: Exposure to AgNPs causes an antiproliferative, apoptotic, and anti-adhesive effect in breast cancer cell lines cultured in vitro. More research is needed to evaluate the potential use of AgNPs to treat different molecular profiles of breast cancer in humans. Keywords: silver nanoparticles, breast cancer, adhesion, apoptosis, transcriptomeRodríguez-Razón CMYañez-Sánchez IRamos-Santillan VOVelásquez-Ordóñez CGutiérrez-Rubio SAGarcía-García MRLópez-Roa RISánchez-Hernández PEDaneri-Navarro AGarcía-Iglesias TDove Medical PressarticleSilver nanoparticlesbreast canceradhesionapoptosisand transcriptome.Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 1081-1095 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Silver nanoparticles breast cancer adhesion apoptosis and transcriptome. Medicine (General) R5-920
spellingShingle	Silver nanoparticles breast cancer adhesion apoptosis and transcriptome. Medicine (General) R5-920 Rodríguez-Razón CM Yañez-Sánchez I Ramos-Santillan VO Velásquez-Ordóñez C Gutiérrez-Rubio SA García-García MR López-Roa RI Sánchez-Hernández PE Daneri-Navarro A García-Iglesias T Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
description	Christian M Rodríguez-Razón,1 Irinea Yañez-Sánchez,2 Vicente O Ramos-Santillan,1 Celso Velásquez-Ordóñez,2 Susan A Gutiérrez-Rubio,1 Maritza R García-García,3 Roció I López-Roa,1 Pedro E Sánchez-Hernández,1 Adrian Daneri-Navarro,1 Trinidad García-Iglesias1 1Laboratory of Immunology and Institute of Experimental and Clinical Therapeutics, Department of Physiology, University Center of Health Sciences, University of Guadalajara, Jalisco, Mexico; 2Center for Research in Nanosciences and Nanotechnology, Department of Natural and Exact Sciences, University Center of the Valleys, University of Guadalajara, Jalisco, Mexico; 3Department of Health Sciences, University Center of the High University of Guadalajara, Jalisco, Mexico Background: Silver nanoparticles (AgNPs) have attracted considerable attention due to the variety of their applications in medicine and other sciences. AgNPs have been used in vitro for treatment of various diseases, such as hepatitis B and herpes simplex infections as well as colon, cervical, and lung cancers. In this study, we assessed the effect on proliferation, adhesion, and apoptosis in breast cancer cell lines of different molecular profiles (MCF7, HCC1954, and HCC70) exposed to AgNPs (2–9 nm).Methods: Breast cancer cell lines were incubated in vitro; MTT assay was used to assess proliferation. Adhesion was determined by real-time analysis with the xCELLingence system. Propidium iodide and fluorescein isothiocyanate-Annexin V assay were used to measure apoptosis. The transcriptome was assessed by gene expression microarray and Probabilistic Graphical Model (PGM) analyses.Results: The results showed a decreased adhesion in breast cancer cell lines and the control exposed to AgNPs was noted in 24 hours (p≤0.05). We observed a significant reduction in the proliferation of MCF7 and HCC70, but not in HCC1954. Apoptotic activity was seen in all cell lines exposed to AgNPs, with an apoptosis percentage of more than 60% in cancer cell lines and less than 60% in the control. PGM analysis confirmed, to some extent, the effects of AgNPs primarily on adhesion by changes in the extracellular matrix.Conclusion: Exposure to AgNPs causes an antiproliferative, apoptotic, and anti-adhesive effect in breast cancer cell lines cultured in vitro. More research is needed to evaluate the potential use of AgNPs to treat different molecular profiles of breast cancer in humans. Keywords: silver nanoparticles, breast cancer, adhesion, apoptosis, transcriptome
format	article
author	Rodríguez-Razón CM Yañez-Sánchez I Ramos-Santillan VO Velásquez-Ordóñez C Gutiérrez-Rubio SA García-García MR López-Roa RI Sánchez-Hernández PE Daneri-Navarro A García-Iglesias T
author_facet	Rodríguez-Razón CM Yañez-Sánchez I Ramos-Santillan VO Velásquez-Ordóñez C Gutiérrez-Rubio SA García-García MR López-Roa RI Sánchez-Hernández PE Daneri-Navarro A García-Iglesias T
author_sort	Rodríguez-Razón CM
title	Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
title_short	Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
title_full	Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
title_fullStr	Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
title_full_unstemmed	Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
title_sort	adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/46dbc3e583fb485fbb0858475002b893
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Adhesion, proliferation, and apoptosis in different molecular portraits of breast cancer treated with silver nanoparticles and its pathway-network analysis

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