Using Live Imaging and FUCCI Embryonic Stem Cells to Rank DevTox Risks: Adverse Growth Effects of PFOA Compared With DEP Are 26 Times Faster, 1,000 Times More Sensitive, and 13 Times Greater in Magnitude

Fluorescent ubiquitination-based cell cycle indicator (FUCCI) embryonic stem cells (ESCs), which fluoresce green during the S-G2-M phases, generate an S-shaped curve for the accumulation of cells during normal stemness (NS) culture with leukemia-inhibitory factor (LIF). Since it was hypothesized tha...

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Bibliographic Details
Main Authors: Mohammed Abdulhasan, Ximena Ruden, Yuan You, Sean M. Harris, Douglas M. Ruden, Awoniyi O. Awonuga, Ayesha Alvero, Elizabeth E. Puscheck, Daniel A. Rappolee
Format: article
Language:EN
Published: Frontiers Media S.A. 2021
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Online Access:https://doaj.org/article/46e2ea619eed4155bd5c4c9d95ff7a82
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