RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors

Repression of gene transcription using CRISPR-Cas9 has been achieved in vitro but not for delivery into adult animal models. Here, the authors use AAV8 to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and show repression up to 24 weeks and reduced cholesterol...

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Autores principales:	Pratiksha I. Thakore, Jennifer B. Kwon, Christopher E. Nelson, Douglas C. Rouse, Matthew P. Gemberling, Matthew L. Oliver, Charles A. Gersbach
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2018
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/46f1559feeb54a51902bab3a11b455bc
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spelling	oai:doaj.org-article:46f1559feeb54a51902bab3a11b455bc2021-12-02T14:38:53ZRNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors10.1038/s41467-018-04048-42041-1723https://doaj.org/article/46f1559feeb54a51902bab3a11b455bc2018-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-018-04048-4https://doaj.org/toc/2041-1723Repression of gene transcription using CRISPR-Cas9 has been achieved in vitro but not for delivery into adult animal models. Here, the authors use AAV8 to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and show repression up to 24 weeks and reduced cholesterol levels in mice.Pratiksha I. ThakoreJennifer B. KwonChristopher E. NelsonDouglas C. RouseMatthew P. GemberlingMatthew L. OliverCharles A. GersbachNature PortfolioarticleScienceQENNature Communications, Vol 9, Iss 1, Pp 1-9 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Pratiksha I. Thakore Jennifer B. Kwon Christopher E. Nelson Douglas C. Rouse Matthew P. Gemberling Matthew L. Oliver Charles A. Gersbach RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
description	Repression of gene transcription using CRISPR-Cas9 has been achieved in vitro but not for delivery into adult animal models. Here, the authors use AAV8 to deliver the transcriptional repressor dSaCas9KRAB to the cholesterol regulator Pcsk9, and show repression up to 24 weeks and reduced cholesterol levels in mice.
format	article
author	Pratiksha I. Thakore Jennifer B. Kwon Christopher E. Nelson Douglas C. Rouse Matthew P. Gemberling Matthew L. Oliver Charles A. Gersbach
author_facet	Pratiksha I. Thakore Jennifer B. Kwon Christopher E. Nelson Douglas C. Rouse Matthew P. Gemberling Matthew L. Oliver Charles A. Gersbach
author_sort	Pratiksha I. Thakore
title	RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
title_short	RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
title_full	RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
title_fullStr	RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
title_full_unstemmed	RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors
title_sort	rna-guided transcriptional silencing in vivo with s. aureus crispr-cas9 repressors
publisher	Nature Portfolio
publishDate	2018
url	https://doaj.org/article/46f1559feeb54a51902bab3a11b455bc
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RNA-guided transcriptional silencing in vivo with S. aureus CRISPR-Cas9 repressors

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