Engineering and optimising deaminase fusions for genome editing

Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.

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Autores principales:	Luhan Yang, Adrian W. Briggs, Wei Leong Chew, Prashant Mali, Marc Guell, John Aach, Daniel Bryan Goodman, David Cox, Yinan Kan, Emal Lesha, Venkataramanan Soundararajan, Feng Zhang, George Church
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2016
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/46f1a4a987da4603908d6583916fecd1
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spelling	oai:doaj.org-article:46f1a4a987da4603908d6583916fecd12021-12-02T16:57:03ZEngineering and optimising deaminase fusions for genome editing10.1038/ncomms133302041-1723https://doaj.org/article/46f1a4a987da4603908d6583916fecd12016-11-01T00:00:00Zhttps://doi.org/10.1038/ncomms13330https://doaj.org/toc/2041-1723Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.Luhan YangAdrian W. BriggsWei Leong ChewPrashant MaliMarc GuellJohn AachDaniel Bryan GoodmanDavid CoxYinan KanEmal LeshaVenkataramanan SoundararajanFeng ZhangGeorge ChurchNature PortfolioarticleScienceQENNature Communications, Vol 7, Iss 1, Pp 1-12 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Luhan Yang Adrian W. Briggs Wei Leong Chew Prashant Mali Marc Guell John Aach Daniel Bryan Goodman David Cox Yinan Kan Emal Lesha Venkataramanan Soundararajan Feng Zhang George Church Engineering and optimising deaminase fusions for genome editing
description	Precision genome engineering using homology donors and the endogenous DNA break repair machinery and recently CRISPR-Cas9 targeted APOBECs have been demonstrated. Here the authors design zinc-finger and TALE chimeric deaminases and investigate editing efficiency and off-target effects.
format	article
author	Luhan Yang Adrian W. Briggs Wei Leong Chew Prashant Mali Marc Guell John Aach Daniel Bryan Goodman David Cox Yinan Kan Emal Lesha Venkataramanan Soundararajan Feng Zhang George Church
author_facet	Luhan Yang Adrian W. Briggs Wei Leong Chew Prashant Mali Marc Guell John Aach Daniel Bryan Goodman David Cox Yinan Kan Emal Lesha Venkataramanan Soundararajan Feng Zhang George Church
author_sort	Luhan Yang
title	Engineering and optimising deaminase fusions for genome editing
title_short	Engineering and optimising deaminase fusions for genome editing
title_full	Engineering and optimising deaminase fusions for genome editing
title_fullStr	Engineering and optimising deaminase fusions for genome editing
title_full_unstemmed	Engineering and optimising deaminase fusions for genome editing
title_sort	engineering and optimising deaminase fusions for genome editing
publisher	Nature Portfolio
publishDate	2016
url	https://doaj.org/article/46f1a4a987da4603908d6583916fecd1
work_keys_str_mv	AT luhanyang engineeringandoptimisingdeaminasefusionsforgenomeediting AT adrianwbriggs engineeringandoptimisingdeaminasefusionsforgenomeediting AT weileongchew engineeringandoptimisingdeaminasefusionsforgenomeediting AT prashantmali engineeringandoptimisingdeaminasefusionsforgenomeediting AT marcguell engineeringandoptimisingdeaminasefusionsforgenomeediting AT johnaach engineeringandoptimisingdeaminasefusionsforgenomeediting AT danielbryangoodman engineeringandoptimisingdeaminasefusionsforgenomeediting AT davidcox engineeringandoptimisingdeaminasefusionsforgenomeediting AT yinankan engineeringandoptimisingdeaminasefusionsforgenomeediting AT emallesha engineeringandoptimisingdeaminasefusionsforgenomeediting AT venkataramanansoundararajan engineeringandoptimisingdeaminasefusionsforgenomeediting AT fengzhang engineeringandoptimisingdeaminasefusionsforgenomeediting AT georgechurch engineeringandoptimisingdeaminasefusionsforgenomeediting
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Engineering and optimising deaminase fusions for genome editing

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