Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.

Soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pathogen of soybean worldwide. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are known to play important role in plant stress response. However, there are few reports profiling the miRNA expression patterns dur...

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Autores principales:	Xiaoyan Li, Xue Wang, Shaopeng Zhang, Dawei Liu, Yuxi Duan, Wei Dong
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/46f2ad0eed6345818d790069c775f6f1
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spelling	oai:doaj.org-article:46f2ad0eed6345818d790069c775f6f12021-11-18T07:14:10ZIdentification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.1932-620310.1371/journal.pone.0039650https://doaj.org/article/46f2ad0eed6345818d790069c775f6f12012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22802924/?tool=EBIhttps://doaj.org/toc/1932-6203Soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pathogen of soybean worldwide. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are known to play important role in plant stress response. However, there are few reports profiling the miRNA expression patterns during pathogen stress. We sequenced four small RNA libraries from two soybean cultivar (Hairbin xiaoheidou, SCN race 3 resistant, Liaodou 10, SCN race 3 susceptible) that grown under un-inoculated and SCN-inoculated soil. Small RNAs were mapped to soybean genome sequence, 364 known soybean miRNA genes were identified in total. In addition, 21 potential miRNA candidates were identified. Comparative analysis of miRNA profiling indicated 101 miRNAs belong to 40 families were SCN-responsive. We also found 20 miRNAs with different express pattern even between two cultivars of the same species. These findings suggest that miRNA paly important role in soybean response to SCN and have important implications for further identification of miRNAs under pathogen stress.Xiaoyan LiXue WangShaopeng ZhangDawei LiuYuxi DuanWei DongPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 6, p e39650 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiaoyan Li Xue Wang Shaopeng Zhang Dawei Liu Yuxi Duan Wei Dong Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
description	Soybean cyst nematode (SCN), Heterodera glycines, is the most devastating pathogen of soybean worldwide. MicroRNAs (miRNAs) are a class of small, non-coding RNAs that are known to play important role in plant stress response. However, there are few reports profiling the miRNA expression patterns during pathogen stress. We sequenced four small RNA libraries from two soybean cultivar (Hairbin xiaoheidou, SCN race 3 resistant, Liaodou 10, SCN race 3 susceptible) that grown under un-inoculated and SCN-inoculated soil. Small RNAs were mapped to soybean genome sequence, 364 known soybean miRNA genes were identified in total. In addition, 21 potential miRNA candidates were identified. Comparative analysis of miRNA profiling indicated 101 miRNAs belong to 40 families were SCN-responsive. We also found 20 miRNAs with different express pattern even between two cultivars of the same species. These findings suggest that miRNA paly important role in soybean response to SCN and have important implications for further identification of miRNAs under pathogen stress.
format	article
author	Xiaoyan Li Xue Wang Shaopeng Zhang Dawei Liu Yuxi Duan Wei Dong
author_facet	Xiaoyan Li Xue Wang Shaopeng Zhang Dawei Liu Yuxi Duan Wei Dong
author_sort	Xiaoyan Li
title	Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
title_short	Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
title_full	Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
title_fullStr	Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
title_full_unstemmed	Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.
title_sort	identification of soybean micrornas involved in soybean cyst nematode infection by deep sequencing.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/46f2ad0eed6345818d790069c775f6f1
work_keys_str_mv	AT xiaoyanli identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing AT xuewang identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing AT shaopengzhang identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing AT daweiliu identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing AT yuxiduan identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing AT weidong identificationofsoybeanmicrornasinvolvedinsoybeancystnematodeinfectionbydeepsequencing
_version_	1718423722354802688

Identification of soybean microRNAs involved in soybean cyst nematode infection by deep sequencing.

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