Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection

An optical biosensor for proteomic breast cancer biomarker detection in complex media is presented. Bloch Surface Waves (BSW) excited onto one dimensional photonic crystal (1DPC) were used to probe the interaction of HER2 with three antibody species and an inert protein (Bovine Serum Albumin - BSA)....

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Autores principales:	Pileri Tommaso, Sinibaldi Alberto, Occhicone Agostino, Giordani Elena, Allegretti Matteo, Munzert Peter, Sonntag Frank, Danz Norbert, Giacomini Patrizio, Michelotti Francesco
Formato:	article
Lenguaje:	EN
Publicado:	EDP Sciences 2021
Materias:	Physics QC1-999
Acceso en línea:	https://doaj.org/article/46f309073910445580275346ba7eda6e
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id	oai:doaj.org-article:46f309073910445580275346ba7eda6e
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spelling	oai:doaj.org-article:46f309073910445580275346ba7eda6e2021-12-02T17:12:51ZOptical multiplexed bioassays on photonic crystals for breast cancer biomarker detection2100-014X10.1051/epjconf/202125513003https://doaj.org/article/46f309073910445580275346ba7eda6e2021-01-01T00:00:00Zhttps://www.epj-conferences.org/articles/epjconf/pdf/2021/09/epjconf_eosam2021_13003.pdfhttps://doaj.org/toc/2100-014XAn optical biosensor for proteomic breast cancer biomarker detection in complex media is presented. Bloch Surface Waves (BSW) excited onto one dimensional photonic crystal (1DPC) were used to probe the interaction of HER2 with three antibody species and an inert protein (Bovine Serum Albumin - BSA). The optical system combines Label-Free readings to track the bioassay real-time development and Fluorescence emission quantification to evaluate the level of specific interaction between the antigen and the antibodies. The results confirm a distinguishable level of affinity between the antibodies and the analyte according to their specificity even at low antibody surface density (about 1173 pg/mm2).Pileri TommasoSinibaldi AlbertoOcchicone AgostinoGiordani ElenaAllegretti MatteoMunzert PeterSonntag FrankDanz NorbertGiacomini PatrizioMichelotti FrancescoEDP SciencesarticlePhysicsQC1-999ENEPJ Web of Conferences, Vol 255, p 13003 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Physics QC1-999
spellingShingle	Physics QC1-999 Pileri Tommaso Sinibaldi Alberto Occhicone Agostino Giordani Elena Allegretti Matteo Munzert Peter Sonntag Frank Danz Norbert Giacomini Patrizio Michelotti Francesco Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
description	An optical biosensor for proteomic breast cancer biomarker detection in complex media is presented. Bloch Surface Waves (BSW) excited onto one dimensional photonic crystal (1DPC) were used to probe the interaction of HER2 with three antibody species and an inert protein (Bovine Serum Albumin - BSA). The optical system combines Label-Free readings to track the bioassay real-time development and Fluorescence emission quantification to evaluate the level of specific interaction between the antigen and the antibodies. The results confirm a distinguishable level of affinity between the antibodies and the analyte according to their specificity even at low antibody surface density (about 1173 pg/mm2).
format	article
author	Pileri Tommaso Sinibaldi Alberto Occhicone Agostino Giordani Elena Allegretti Matteo Munzert Peter Sonntag Frank Danz Norbert Giacomini Patrizio Michelotti Francesco
author_facet	Pileri Tommaso Sinibaldi Alberto Occhicone Agostino Giordani Elena Allegretti Matteo Munzert Peter Sonntag Frank Danz Norbert Giacomini Patrizio Michelotti Francesco
author_sort	Pileri Tommaso
title	Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
title_short	Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
title_full	Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
title_fullStr	Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
title_full_unstemmed	Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
title_sort	optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection
publisher	EDP Sciences
publishDate	2021
url	https://doaj.org/article/46f309073910445580275346ba7eda6e
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Optical multiplexed bioassays on photonic crystals for breast cancer biomarker detection

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